AB137080

Anti-TROY antibody [EPR3214(2)]

RabMAb
Recombinant
What is this?

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(1 Publication)

Rabbit Recombinant Monoclonal TROY antibody. Suitable for WB, Flow Cyt (Intra) and reacts with Human samples. Cited in 1 publication.

View Alternative Names

TAJ, TROY, UNQ1888/PRO4333, TNFRSF19, Tumor necrosis factor receptor superfamily member 19, TRADE, Toxicity and JNK inducer

3 Images

Flow Cytometry (Intracellular) - Anti-TROY antibody [EPR3214(2)] (AB137080)

Flow Cyt (Intra)

Unknown

Flow Cytometry (Intracellular) - Anti-TROY antibody [EPR3214(2)] (AB137080)

Intracellular flow cytometric analysis of permeabilized A431 cells labelling TROY with ab137080 at 1/100 dilution (red) or a rabbit IgG (negative) (green).

Lab

Western blot - Anti-TROY antibody [EPR3214(2)] (AB137080)

Blocking buffer and concentration : 5% NFDM/TBST
Diluting buffer and concentration : 5% NFDM/TBST

All lanes:

Western blot - Anti-TROY antibody [EPR3214(2)] (ab137080) at 1/10000 dilution

Lane 1:

RAW 264.7 (Mouse Abelson murine leukemia virus-induced tumor macrophage) without membrane fraction lysate at 20 µg

Lane 2:

RAW 264.7 (Mouse Abelson murine leukemia virus-induced tumor macrophage) membrane fraction lysate at 20 µg

Lane 3:

THP-1 (Human monocytic leukemia monocyte) without membrane fraction lysate at 20 µg

Lane 4:

THP-1 (Human monocytic leukemia monocyte) membrane fraction lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 46 kDa

Observed band size: 45 kDa

false

Exposure time: 120s

Unknown

Western blot - Anti-TROY antibody [EPR3214(2)] (AB137080)

All lanes:

Western blot - Anti-TROY antibody [EPR3214(2)] (ab137080) at 1/1000 dilution

Lane 1:

LnCaP cell lysates at 10 µg

Lane 2:

A431 cell lysates at 10 µg

Lane 3:

PC3 cell lysates at 10 µg

Secondary

All lanes:

goat anti-rabbit HRP at 1/2000 dilution

Predicted band size: 46 kDa

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR3214(2)

Isotype

IgG

Carrier free

Reacts with

Human

Applications

WB, Flow Cyt (Intra)

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/100 - 1/500", "FlowCytIntra-species-notes": "<a href='/en-us/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-ab172730'>ab172730</a> - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody." }, "Mouse": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Rat": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The TROY protein also known as TNFRSF19 is a member of the tumor necrosis factor receptor superfamily. It has a molecular mass of approximately 42 kDa. TROY is mainly expressed in the central nervous system particularly in brain tissue but researchers also detect it in certain peripheral tissues. The protein contains an extracellular domain a single transmembrane segment and a cytoplasmic domain that interacts with other intracellular signaling proteins. These structural components allow it to function effectively in signal transduction throughout the cells.

Biological function summary

TROY protein plays a significant role in the central nervous system's development and maintenance. It interacts with components of the neurotrophin signaling pathway including p75 neurotrophin receptor to influence neuronal survival and differentiation. TROY does not function alone; it is part of receptor complexes that modulate various intracellular pathways. Its interactions with ligands like Nogo receptor and LINGO-1 indicate its involvement in restricting neuronal growth impacting axon regeneration and central nervous system repair processes.

Pathways

The TROY protein integrates into neurotrophin signaling and axon guidance pathways. In neurotrophin signaling it affects cellular response to growth factors essential for neural growth and survival. In axon guidance TROY impacts cellular navigation cues that direct axon growth. This protein interacts closely with other molecules such as the Nogo receptor which often collaborates in signaling cascades that influence these pathways. These interactions underline TROY's contribution to maintaining the delicate balance of neuronal connections.

TROY protein has links to neurodegenerative disorders like Alzheimer's disease and multiple sclerosis. Alzheimer's disease research shows that TROY might influence amyloid-beta peptide interactions exacerbating neuronal degradation processes. Similarly in multiple sclerosis the protein's role in inhibiting axon regrowth makes it a potential target for therapeutic intervention. TROY's interaction with proteins such as p75 and Nogo receptor highlights its influence on disease progression by mediating cellular processes that relate to neurodegeneration and neuron damage response.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Can mediate activation of JNK and NF-kappa-B. May promote caspase-independent cell death.

See full target information TNFRSF19

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Oncotarget 8:50557-50569 PubMed28881583

2016

Troy is expressed in human stomach mucosa and a novel putative prognostic marker of intestinal type gastric cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Franziska Wilhelm,Christine Böger,Sandra Krüger,Hans-Michael Behrens,Christoph Röcken

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-TROY antibody [EPR3214(2)]

Flow Cytometry (Intracellular) - Anti-TROY antibody [EPR3214(2)] (AB137080)

Western blot - Anti-TROY antibody [EPR3214(2)] (AB137080)

Western blot - Anti-TROY antibody [EPR3214(2)] (AB137080)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Complementary Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

Product promise