AB186929

Anti-TRP1 antibody [EPR13063] - BSA and Azide free

RabMAb
Recombinant
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(2 Publications)

Rabbit Recombinant Monoclonal TRP1 antibody. Carrier free. Suitable for WB, ICC/IF, Flow Cyt (Intra), IHC-P and reacts with Mouse, Human samples. Cited in 2 publications.

View Alternative Names

CAS2, TYRP, TYRRP, TYRP1, DHICA oxidase, Catalase B, Glycoprotein 75, Melanoma antigen gp75, Tyrosinase-related protein 1, TRP, TRP-1, TRP1

5 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRP1 antibody [EPR13063] - BSA and Azide free (AB186929)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRP1 antibody [EPR13063] - BSA and Azide free (AB186929)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human skin tissue sections labeling TRP1 with purified ab178676 at 1/1000 dilution (0.36 μg/ml). Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0). ImmunoHistoProbe one step HRP Polymer (ready to use) was used as the secondary antibody. Negative control : PBS instead of the primary antibody. Hematoxylin was used as a counterstain.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab178676).

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRP1 antibody [EPR13063] - BSA and Azide free (AB186929)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRP1 antibody [EPR13063] - BSA and Azide free (AB186929)

Immunohistochemical analysis of paraffin-embedded Human skin tissue labeling TRP1 with ab178676 (unpurified) at 1/100 dilution.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab178676).

Immunocytochemistry/ Immunofluorescence - Anti-TRP1 antibody [EPR13063] - BSA and Azide free (AB186929)

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-TRP1 antibody [EPR13063] - BSA and Azide free (AB186929)

Immunocytochemistry/ Immunofluorescence analysis of B16-F10 (Mouse melanoma epithelial cell-like) cells labeling TRP1 with purified ab178676 at 1/100 dilution (3.6 μg/ml). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor^® 594) at 1/200 (2.5 μg/ml). Goat anti rabbit IgG (Alexa Fluor^® 488, ab150077) was used as the secondary antibody at 1/1000 (2 μg/ml) dilution. DAPI (blue) was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab178676).

Flow Cytometry (Intracellular) - Anti-TRP1 antibody [EPR13063] - BSA and Azide free (AB186929)

Flow Cyt (Intra)

Unknown

Flow Cytometry (Intracellular) - Anti-TRP1 antibody [EPR13063] - BSA and Azide free (AB186929)

Intracellular Flow Cytometry analysis of B16-F0 (Mouse melanoma epithelial cell-like) cells labeling TRP1 with purified ab178676 at 1/40 dilution (10μg/ml) (red). Cells were fixed with 4% Paraformaldehyde and permeabilised with 90% Methanol. A Goat anti rabbit IgG (Alexa Fluor^® 488, ab150077) secondary antibody was used at 1/2000. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue).

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab178676).

Immunocytochemistry/ Immunofluorescence - Anti-TRP1 antibody [EPR13063] - BSA and Azide free (AB186929)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-TRP1 antibody [EPR13063] - BSA and Azide free (AB186929)

Immunofluorescent analysis of B16-F0 cells labeling TRP1 with ab178676 (unpurified) at 1/100 dilution.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab178676).

Unconjugated

Anti-TRP1 antibody [EPR13063]
660 APC

APC Anti-TRP1 antibody [EPR13063]
519 Alexa Fluor® 488

Alexa Fluor® 488 Anti-TRP1 antibody [EPR13063]
565 Alexa Fluor® 555

Alexa Fluor® 555 Anti-TRP1 antibody [EPR13063]
603 Alexa Fluor® 568

Alexa Fluor® 568 Anti-TRP1 antibody [EPR13063]
617 Alexa Fluor® 594

Alexa Fluor® 594 Anti-TRP1 antibody [EPR13063]
665 Alexa Fluor® 647

Alexa Fluor® 647 Anti-TRP1 antibody [EPR13063]
775 Alexa Fluor® 750

Alexa Fluor® 750 Anti-TRP1 antibody [EPR13063]
HRP

HRP Anti-TRP1 antibody [EPR13063]
578 PE

PE Anti-TRP1 antibody [EPR13063]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR13063

Isotype

IgG

Carrier free

Yes

Reacts with

Mouse, Human

Applications

WB, ICC/IF, Flow Cyt (Intra), IHC-P

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" } } }

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Product details

ab186929 is the carrier-free version of ab178676.

This antibody is unsuitable for IHC-P on mouse samples.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Species reactivity
Rat: We have preliminary internal testing data to indicate this antibody may not react with this species.
Please contact us for more information.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

Do Not Freeze

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

TRP1 (Tyrosinase-related protein 1) also known as gp75 is a copper-binding protein involved in melanin biosynthesis. TRP1 has a molecular mass of approximately 75 kDa. It is expressed mainly in melanocytes and retinal pigment epithelium cells where it contributes to pigmentation processes. TRP1 functions as an oxidase enzyme catalyzing steps in the transformation of dopa to melanin a pigment responsible for coloration in skin hair and eyes.

Biological function summary

TRP1 participates in a protein complex known as the melanosome. In this complex it plays a role in the proper polymerization and stabilization of eumelanin one type of melanin. It influences the quality and quantity of melanin produced affecting pigmentation. TRP1’s interaction with other tyrosinase family members like tyrosinase and TRP2 is important in maintaining the complex's integrity and function with each member contributing uniquely to the melanogenesis process.

Pathways

TRP1 operates within the melanogenesis and WNT signaling pathways. In the melanogenesis pathway TRP1 works alongside tyrosinase for the production of melanin pigment. The WNT signaling pathway which is significant for cellular differentiation and proliferation connects TRP1 through regulatory networks that influence melanocyte development and pigmentation. Regulation of TRP1 and these proteins ensures proper melanin synthesis impacting pigmentation processes in the organism.

TRP1 has connections to conditions such as albinism and melanoma. In albinism mutations in TRP1 can lead to reduced melanin production causing pigmentation defects. TRP1's altered expression has been linked to melanoma a type of skin cancer highlighting its role in melanin regulation and tumor biology. The relationship between TRP1 and tyrosinase in diseases like melanoma highlights the significance of their interaction in both normal pigmentation and disease progression.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Plays a role in melanin biosynthesis (PubMed : 16704458, PubMed : 22556244, PubMed : 23504663). Catalyzes the oxidation of 5,6-dihydroxyindole-2-carboxylic acid (DHICA) into indole-5,6-quinone-2-carboxylic acid in the presence of bound Cu(2+) ions, but not in the presence of Zn(2+) (PubMed : 28661582). May regulate or influence the type of melanin synthesized (PubMed : 16704458, PubMed : 22556244). Also to a lower extent, capable of hydroxylating tyrosine and producing melanin (By similarity).

See full target information TYRP1

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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Nature immunology 26:1182-1197 PubMed40588561

2025

Chemosensor receptors are lipid-detecting regulators of macrophage function in cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Giulia Marelli,Nicolò Morina,Simone Puccio,Marta Iovino,Marta Pandini,Federica Portale,Mattia Carvetta,Divya Mishra,Elisabetta Diana,Greta Meregalli,Elvezia Paraboschi,Javier Cibella,Clelia Peano,Gianluca Basso,Gabriele De Simone,Chiara Camisaschi,Elena Magrini,Giulio Sartori,Elham Karimi,Piergiuseppe Colombo,Massimo Lazzeri,Paolo Casale,Lavinia Morosi,Giuseppe Martano,Rosanna Asselta,Eduardo Bonavita,Hiro Matsunami,Francesco Bertoni,Logan Walsh,Enrico Lugli,Diletta Di Mitri

Experimental dermatology 30:578-587 PubMed33598985

2021

Stress-associated ectopic differentiation of melanocyte stem cells and ORS amelanotic melanocytes in an ex vivo human hair follicle model.

Applications

Unspecified application

Species

Unspecified reactive species

Inbal Rachmin,Ju Hee Lee,Bing Zhang,James Sefton,Inhee Jung,Young In Lee,Ya-Chieh Hsu,David E Fisher

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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