Rabbit Recombinant Monoclonal TRP1 antibody. Carrier free. Suitable for IHC-P, IP, WB and reacts with Human samples.
Images
![Western blot - Anti-TRP1 antibody [EPR13064(B)] - BSA and Azide free (AB249974), expandable thumbnail](https://content.abcam.com/products/images/trp1-antibody-epr13064b-bsa-and-azide-free-ab249974--western-blot-img72994.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-TRP1 antibody [EPR13064(B)] - BSA and Azide free (AB249974)")
![Immunoprecipitation - Anti-TRP1 antibody [EPR13064(B)] - BSA and Azide free (AB249974), expandable thumbnail](https://content.abcam.com/products/images/trp1-antibody-epr13064b-bsa-and-azide-free-ab249974--immunoprecipitation-img144566.jpg/_jcr_content/renditions/webp-475.webp "Immunoprecipitation - Anti-TRP1 antibody [EPR13064(B)] - BSA and Azide free (AB249974)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRP1 antibody [EPR13064(B)] - BSA and Azide free (AB249974), expandable thumbnail](https://content.abcam.com/products/images/trp1-antibody-epr13064b-bsa-and-azide-free-ab249974--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img33475.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRP1 antibody [EPR13064(B)] - BSA and Azide free (AB249974)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRP1 antibody [EPR13064(B)] - BSA and Azide free (AB249974), expandable thumbnail](https://content.abcam.com/products/images/trp1-antibody-epr13064b-bsa-and-azide-free-ab249974--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img108288.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRP1 antibody [EPR13064(B)] - BSA and Azide free (AB249974)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IHC-P | IP | WB | ICC/IF | |
|---|---|---|---|---|
| Human | Tested | Tested | Tested | Not recommended |
| Mouse | Not recommended | Not recommended | Not recommended | Not recommended |
| Rat | Not recommended | Not recommended | Not recommended | Not recommended |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Species Rat | Dilution info - | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
IP
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
ICC/IF
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Human | Dilution info - | Notes - |
Target data
Function
Plays a role in melanin biosynthesis (PubMed:16704458, PubMed:22556244, PubMed:23504663). Catalyzes the oxidation of 5,6-dihydroxyindole-2-carboxylic acid (DHICA) into indole-5,6-quinone-2-carboxylic acid in the presence of bound Cu(2+) ions, but not in the presence of Zn(2+) (PubMed:28661582). May regulate or influence the type of melanin synthesized (PubMed:16704458, PubMed:22556244). Also to a lower extent, capable of hydroxylating tyrosine and producing melanin (By similarity).
Alternative names
CAS2, TYRP, TYRRP, TYRP1, DHICA oxidase, Catalase B, Glycoprotein 75, Melanoma antigen gp75, Tyrosinase-related protein 1, TRP, TRP-1, TRP1
Recommended products
Rabbit Recombinant Monoclonal TRP1 antibody. Carrier free. Suitable for IHC-P, IP, WB and reacts with Human samples.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
- Yes
- Clone number
- EPR13064(B)
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- Do Not Freeze
Notes
ab249974 is the carrier-free version of Anti-TRP1 antibody [EPR13064(B)] - C-terminal ab177468.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
TRP1 (Tyrosinase-related protein 1) also known as gp75 is a copper-binding protein involved in melanin biosynthesis. TRP1 has a molecular mass of approximately 75 kDa. It is expressed mainly in melanocytes and retinal pigment epithelium cells where it contributes to pigmentation processes. TRP1 functions as an oxidase enzyme catalyzing steps in the transformation of dopa to melanin a pigment responsible for coloration in skin hair and eyes.
- Biological function summary
TRP1 participates in a protein complex known as the melanosome. In this complex it plays a role in the proper polymerization and stabilization of eumelanin one type of melanin. It influences the quality and quantity of melanin produced affecting pigmentation. TRP1’s interaction with other tyrosinase family members like tyrosinase and TRP2 is important in maintaining the complex's integrity and function with each member contributing uniquely to the melanogenesis process.
- Pathways
TRP1 operates within the melanogenesis and WNT signaling pathways. In the melanogenesis pathway TRP1 works alongside tyrosinase for the production of melanin pigment. The WNT signaling pathway which is significant for cellular differentiation and proliferation connects TRP1 through regulatory networks that influence melanocyte development and pigmentation. Regulation of TRP1 and these proteins ensures proper melanin synthesis impacting pigmentation processes in the organism.
- Associated diseases and disorders
TRP1 has connections to conditions such as albinism and melanoma. In albinism mutations in TRP1 can lead to reduced melanin production causing pigmentation defects. TRP1's altered expression has been linked to melanoma a type of skin cancer highlighting its role in melanin regulation and tumor biology. The relationship between TRP1 and tyrosinase in diseases like melanoma highlights the significance of their interaction in both normal pigmentation and disease progression.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
4 product images
![Western blot - Anti-TRP1 antibody [EPR13064(B)] - BSA and Azide free (ab249974), expandable thumbnail](https://content.abcam.com/products/images/trp1-antibody-epr13064b-bsa-and-azide-free-ab249974--western-blot-img72994.jpg "Western blot - Anti-TRP1 antibody [EPR13064(B)] - BSA and Azide free (ab249974)")
Western blot - Anti-TRP1 antibody [EPR13064(B)] - BSA and Azide free (ab249974)
This data was developed using Anti-TRP1 antibody [EPR13064(B)] - C-terminal ab177468, the same antibody clone in a different buffer formulation.
All lanes: Western blot - Anti-TRP1 antibody [EPR13064(B)] - C-terminal (Anti-TRP1 antibody [EPR13064(B)] - C-terminal ab177468) at 1/1000 dilution
Lane 1: Human melanoma tissue lysate at 10 µg
Lane 2: Human skin tissue lysate at 10 µg
SecondaryAll lanes: Goat anti-rabbit HRP conjugated at 1/2000 dilution
Developed using the ECL technique.
Predicted band size: 61 kDa
![Immunoprecipitation - Anti-TRP1 antibody [EPR13064(B)] - BSA and Azide free (ab249974), expandable thumbnail](https://content.abcam.com/products/images/trp1-antibody-epr13064b-bsa-and-azide-free-ab249974--immunoprecipitation-img144566.jpg "Immunoprecipitation - Anti-TRP1 antibody [EPR13064(B)] - BSA and Azide free (ab249974)")
Immunoprecipitation - Anti-TRP1 antibody [EPR13064(B)] - BSA and Azide free (ab249974)
This data was developed using Anti-TRP1 antibody [EPR13064(B)] - C-terminal ab177468, the same antibody clone in a different buffer formulation.
Western blot analysis on immunoprecipitation pellet from fetal skin lysate using Anti-TRP1 antibody [EPR13064(B)] - C-terminal ab177468 at 1/10 dilution.
All lanes: Immunoprecipitation - Anti-TRP1 antibody [EPR13064(B)] - C-terminal (Anti-TRP1 antibody [EPR13064(B)] - C-terminal ab177468)
Developed using the ECL technique.
Predicted band size: 61 kDa
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRP1 antibody [EPR13064(B)] - BSA and Azide free (ab249974), expandable thumbnail](https://content.abcam.com/products/images/trp1-antibody-epr13064b-bsa-and-azide-free-ab249974--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img33475.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRP1 antibody [EPR13064(B)] - BSA and Azide free (ab249974)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRP1 antibody [EPR13064(B)] - BSA and Azide free (ab249974)
This data was developed using Anti-TRP1 antibody [EPR13064(B)] - C-terminal ab177468, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded Human melanoma tissue labeling TRP1 with Anti-TRP1 antibody [EPR13064(B)] - C-terminal ab177468 at 1/50 diultion. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRP1 antibody [EPR13064(B)] - BSA and Azide free (ab249974), expandable thumbnail](https://content.abcam.com/products/images/trp1-antibody-epr13064b-bsa-and-azide-free-ab249974--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img108288.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRP1 antibody [EPR13064(B)] - BSA and Azide free (ab249974)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRP1 antibody [EPR13064(B)] - BSA and Azide free (ab249974)
This data was developed using Anti-TRP1 antibody [EPR13064(B)] - C-terminal ab177468, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded Human skin tissue labeling TRP1 with Anti-TRP1 antibody [EPR13064(B)] - C-terminal ab177468 at 1/50 diultion. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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