Rabbit Polyclonal TRP2/DCT antibody. Suitable for IHC-P, WB and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human DCT aa 250-450.
Images
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine)- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Recombinant Fragment Protein within Human DCT aa 250-450. The exact immunogen used to generate this antibody is proprietary information. Database link P40126
Reactivity data
Select an application| IHC-P | WB | |
|---|---|---|
| Human | Tested | Tested |
| Pig | Predicted | Predicted |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/200.00000 - 1/500.00000 | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Pig | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 0.04000-0.40000 µg/mL | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Pig | Dilution info - | Notes - |
Associated Products
Select an associated product type
3 products for Alternative Product
Target data
Function
Plays a role in melanin biosynthesis (PubMed:33100333). Catalyzes the conversion of L-dopachrome into 5,6-dihydroxyindole-2-carboxylic acid (DHICA).
Alternative names
TYRP2, DCT, L-dopachrome tautomerase, DT, L-dopachrome Delta-isomerase, Tyrosinase-related protein 2, TRP-2, TRP2
Recommended products
Rabbit Polyclonal TRP2/DCT antibody. Suitable for IHC-P, WB and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human DCT aa 250-450.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine)- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Recombinant Fragment Protein within Human DCT aa 250-450. The exact immunogen used to generate this antibody is proprietary information. Database link P40126
- Purification technique
- Affinity purification
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
TRP2 also known as DCT or dopachrome tautomerase is a protein involved in melanin synthesis and is part of the tyrosinase-related protein family. It has a molecular weight of approximately 60 kDa. TRP2/DCT is mainly expressed in melanocytes which are cells responsible for pigment production. The protein functions in the melanin biosynthetic pathway converting dopachrome into DHICA contributing to the eumelanin formation which impacts skin and hair color.
- Biological function summary
TRP2/DCT participates in pigmentation and photoprotection processes. It functions in tandem with other enzymes like tyrosinase and TRP1 to form a multiprotein complex important for melanin synthesis. The coordination of these proteins ensures the effective production and regulation of melanin which absorbs ultraviolet radiation and reduces DNA damage in skin cells.
- Pathways
TRP2/DCT plays a role in the melanin biosynthetic pathway and influences the Wnt signaling pathway. The melanin pathway involves enzymes such as tyrosinase which initiates the conversion of tyrosine to L-DOPA and eventually to melanin pigments. The Wnt signaling pathway critical for cellular processes like proliferation and differentiation interacts with melanogenesis through proteins like beta-catenin influencing TRP2/DCT expression and activity.
- Associated diseases and disorders
TRP2/DCT has significant associations with melanoma and vitiligo. In melanoma altered expression of TRP2/DCT can impact tumor cell proliferation and survival often interacting with proteins like MITF an important regulator of melanocyte development. In vitiligo changes in TRP2/DCT expression can disrupt melanin production leading to depigmented patches on the skin. Understanding these interactions and expressions aids in potential therapeutic approaches for these skin conditions.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
7 product images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRP2/DCT antibody (ab223736)
Immunohistochemical analysis of human skin tissue labeling TRP2/DCT (high expression) with ab223736 at 1/200 dilution.
Performed heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Western blot - Anti-TRP2/DCT antibody (ab223736)
All lanes: Western blot - Anti-TRP2/DCT antibody (ab223736) at 0.4 µg/mL
Lane 1: SK-MEL-30 (human cutaneous melanoma cell line) whole cell lysate
Lane 2: U-2 OS (human bone osteosarcoma epithelial cell line) whole cell lysate
Predicted band size: 59 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRP2/DCT antibody (ab223736)
Immunohistochemical analysis of human skin tissue labeling TRP2/DCT with ab223736 at 1/200 dilution.
Performed heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRP2/DCT antibody (ab223736)
Immunohistochemical analysis of human skeletal muscle tissue labeling TRP2/DCT (low expression) with ab223736 at 1/200 dilution.
Performed heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRP2/DCT antibody (ab223736)
Immunohistochemical analysis of human testis tissue labeling TRP2/DCT with ab223736 at 1/200 dilution.
Performed heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRP2/DCT antibody (ab223736)
Immunohistochemical analysis of human cerebral cortex tissue labeling TRP2/DCT with ab223736 at 1/200 dilution.
Performed heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRP2/DCT antibody (ab223736)
Immunohistochemical analysis of human lumph node tissue labeling TRP2/DCT with ab223736 at 1/200 dilution.
Performed heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com