AB74073

Anti-TRP2/DCT antibody

Name: Anti-TRP2/DCT antibody (ab74073) | Abcam
Brand: Abcam Limited
SKU: AB74073
Price: 500 USD
Availability: InStock
Rating: 5 (9 reviews)

(9 Reviews)

(73 Publications)

Anti-TRP2/DCT antibody (ab74073) is a rabbit polyclonal antibody detecting TRP2/DCT in Western Blot, IHC-P, IHC-Fr, ICC/IF, ELISA. Suitable for Human, Mouse.

- Over 50 publications
- Trusted since 2009

View Alternative Names

TYRP2, DCT, L-dopachrome tautomerase, DT, L-dopachrome Delta-isomerase, Tyrosinase-related protein 2, TRP-2, TRP2

3 Images

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Western blot - Anti-TRP2/DCT antibody (AB74073)

All lanes:

Western blot - Anti-TRP2/DCT antibody (ab74073) at 1/500 dilution

Lane 1:

Extracts from K562 cells at 5 µg with no peptide

Lane 2:

Extracts from K562 cells at 5 µg with peptide

Predicted band size: 59 kDa

Observed band size: 117 kDa,39 kDa,59 kDa

false

Immunocytochemistry/ Immunofluorescence - Anti-TRP2/DCT antibody (AB74073)

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-TRP2/DCT antibody (AB74073)

Immunofluorescence analysis of TRP2/DCT in A549 cells, using ab74073 at a dilution of 1/500.
Left hand panel : no peptide
right hand panel : with immunising peptide.

ICC/IF

AbReview38338****

Immunocytochemistry/ Immunofluorescence - Anti-TRP2/DCT antibody (AB74073)

ab74073 staining TRP2/DCT in Mouse melan-ink melanocytes by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde and blocked with 5% serum for 30 hours at room temperature. Samples were incubated with primary antibody (1/100 in PBS -T+ 1% BSA) for 12 hours. An undiluted Alexa Fluor® 488-conjugated Goat anti-rabbit IgG polyclonal was used as the secondary antibody.

This image is courtesy of an Abreview submitted by Heiko Locher

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human, Mouse

Applications

ELISA, WB, IHC-P, ICC/IF, IHC-Fr

applications

Immunogen

Synthetic Peptide within Human DCT. The exact immunogen used to generate this antibody is proprietary information.

P40126

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ELISA" : {"fullname" : "ELISA", "shortname":"ELISA"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IHCFr" : {"fullname" : "Immunohistochemistry (Frozen sections)", "shortname":"IHC-Fr"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ELISA-species-checked": "guaranteed", "ELISA-species-dilution-info": "", "ELISA-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/1000", "WB-species-notes": "TRP2 protein is a single-pass type I membrane protein, these types of proteins are known to aggregate when boiled, therefore, we recommend heating the lysates in sample buffer for 15-20 minutes at 60-70°C instead of boiling.", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/500", "ICCIF-species-notes": "", "IHCFr-species-checked": "guaranteed", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "" }, "Mouse": { "ELISA-species-checked": "guaranteed", "ELISA-species-dilution-info": "", "ELISA-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCFr-species-checked": "guaranteed", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "" } } }

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Product details

What is this antibody validated in?
Anti-TRP2/DCT antibody (ab74073) is a rabbit polyclonal antibody and is validated for use in Western Blot (WB), Immunohistochemistry (IHC-P), Immunohistochemistry (IHC-Fr), Immunocytochemistry/immunofluorescence (ICC/IF), ELISA in Human, Mouse samples.

What is the molecular weight of TRP2/DCT?
Anti-TRP2/DCT (ab74073) specifically detects a band for TRP2/DCT (UniProt: P40126) at a molecular weight of 59kDa.

Trusted by the scientific community
Anti-TRP2/DCT (ab74073) was first used in a scientific publication in 2009 and has been cited over 50 times in peer-reviewed journals.

Reviewed by scientists
Anti-TRP2/DCT (ab74073) has over 5 independent reviews from customers.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Purification notes

The antibody was affinity purified from rabbit antiserum by affinity chromatography using epitope specific immunogen.

Storage buffer

pH: 7 Preservative: 0.02% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

TRP2 also known as DCT or dopachrome tautomerase is a protein involved in melanin synthesis and is part of the tyrosinase-related protein family. It has a molecular weight of approximately 60 kDa. TRP2/DCT is mainly expressed in melanocytes which are cells responsible for pigment production. The protein functions in the melanin biosynthetic pathway converting dopachrome into DHICA contributing to the eumelanin formation which impacts skin and hair color.

Biological function summary

TRP2/DCT participates in pigmentation and photoprotection processes. It functions in tandem with other enzymes like tyrosinase and TRP1 to form a multiprotein complex important for melanin synthesis. The coordination of these proteins ensures the effective production and regulation of melanin which absorbs ultraviolet radiation and reduces DNA damage in skin cells.

Pathways

TRP2/DCT plays a role in the melanin biosynthetic pathway and influences the Wnt signaling pathway. The melanin pathway involves enzymes such as tyrosinase which initiates the conversion of tyrosine to L-DOPA and eventually to melanin pigments. The Wnt signaling pathway critical for cellular processes like proliferation and differentiation interacts with melanogenesis through proteins like beta-catenin influencing TRP2/DCT expression and activity.

TRP2/DCT has significant associations with melanoma and vitiligo. In melanoma altered expression of TRP2/DCT can impact tumor cell proliferation and survival often interacting with proteins like MITF an important regulator of melanocyte development. In vitiligo changes in TRP2/DCT expression can disrupt melanin production leading to depigmented patches on the skin. Understanding these interactions and expressions aids in potential therapeutic approaches for these skin conditions.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Plays a role in melanin biosynthesis (PubMed : 33100333). Catalyzes the conversion of L-dopachrome into 5,6-dihydroxyindole-2-carboxylic acid (DHICA).

See full target information DCT

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Publications (73)

Recent publications for all applications. Explore the full list and refine your search

Cell regeneration (London, England) 14:25 PubMed40522608

2025

Mitochondrial deoxyguanosine kinase depletion induced ROS causes melanocyte stem cell exhaustion and hair greying.

Applications

Unspecified application

Species

Unspecified reactive species

Kaiyao Zhou,Gangyun Wu,Rui Dong,Changhao Kan,Lin Xie,Lijuan Gao,Hua Li,Jianwei Sun,Wenxiu Ning

Methods in molecular biology (Clifton, N.J.) 2922:21-33 PubMed40208524

2025

Isolation and Culture of Epidermal Melanocytes.

Applications

Unspecified application

Species

Unspecified reactive species

Muriel Cario,Alain Taieb

Cancer & metabolism 13:5 PubMed39875968

2025

Steatohepatitis-induced vascular niche alterations promote melanoma metastasis.

Applications

Unspecified application

Species

Unspecified reactive species

Johannes Hoffmann,Julia Schüler,Bianca Dietsch,Sina Wietje Kürschner-Zacharias,Carsten Sticht,Felix A Trogisch,Maren Schreitmüller,Tinja Baljkas,Kai Schledzewski,Manuel Reinhart,Sebastian A Wohlfeil,Manuel Winkler,Christian David Schmid,Joerg Heineke,Cyrill Géraud,Sergij Goerdt,Philipp-Sebastian Reiners-Koch,Victor Olsavszky

PLoS biology 22:e3002895 PubMed39527653

2024

Mitochondrial calcium uptake orchestrates vertebrate pigmentation via transcriptional regulation of keratin filaments.

Applications

Unspecified application

Species

Unspecified reactive species

Jyoti Tanwar,Kriti Ahuja,Akshay Sharma,Paras Sehgal,Gyan Ranjan,Farina Sultan,Anushka Agrawal,Donato D'Angelo,Anshu Priya,Vamsi K Yenamandra,Archana Singh,Anna Raffaello,Muniswamy Madesh,Rosario Rizzuto,Sridhar Sivasubbu,Rajender K Motiani

Pharmaceuticals (Basel, Switzerland) 17: PubMed39458943

2024

Apigenin Ameliorates HO-Induced Oxidative Damage in Melanocytes through Nuclear Factor-E2-Related Factor 2 (Nrf2) and Phosphatidylinositol 3-Kinase (PI3K)/Protein Kinase B (Akt)/Mammalian Target of Rapamycin (mTOR) Pathways and Reducing the Generation of Reactive Oxygen Species (ROS) in Zebrafish.

Applications

Unspecified application

Species

Unspecified reactive species

Qing-Qing Tang,Zu-Ding Wang,Xiao-Hong An,Xin-Yuan Zhou,Rong-Zhan Zhang,Xiao Zhan,Wei Zhang,Jia Zhou

American journal of human genetics 111:2265-2282 PubMed39293448

2024

Congenital microcoria deletion in mouse links Sox21 dysregulation to disease and suggests a role for TGFB2 in glaucoma and myopia.

Applications

Unspecified application

Species

Unspecified reactive species

Elisa Erjavec,Clémentine Angée,Djihad Hadjadj,Bruno Passet,Pierre David,Corinne Kostic,Emmanuel Dodé,Xavier Zanlonghi,Nicolas Cagnard,Brigitte Nedelec,Sylvain V Crippa,Christine Bole-Feysot,Mohammed Zarhrate,Sophie Creuzet,Johan Castille,Jean-Luc Vilotte,Patrick Calvas,Julie Plaisancié,Nicolas Chassaing,Josseline Kaplan,Jean-Michel Rozet,L Fares Taie

Cancer immunology, immunotherapy : CII 73:178 PubMed38954031

2024

TRP-2 / gp100 DNA vaccine and PD-1 checkpoint blockade combination for the treatment of intracranial tumors.

Applications

Unspecified application

Species

Unspecified reactive species

Joshua R D Pearson,Carles Puig-Saenz,Jubini E Thomas,Lydia D Hardowar,Murrium Ahmad,Louise C Wainwright,Adam M McVicar,Victoria A Brentville,Chris J Tinsley,A Graham Pockley,Lindy G Durrant,Stephanie E B McArdle

Antioxidants (Basel, Switzerland) 13: PubMed38929152

2024

Anti-Melanogenic Activity of Ethanolic Extract from Fruits Using In Vitro Experiments, Network Pharmacology, Molecular Docking, and Molecular Dynamics Simulation.

Applications

Unspecified application

Species

Unspecified reactive species

Aman Tedasen,Anchalee Chiabchalard,Tewin Tencomnao,Kenshi Yamasaki,Hideyuki J Majima,Atthaphong Phongphithakchai,Moragot Chatatikun

Cells 13: PubMed38891124

2024

Establishment of Canine Oral Mucosal Melanoma Cell Lines and Their Xenogeneic Animal Models.

Applications

Unspecified application

Species

Unspecified reactive species

Shumin Li,Zichen Liu,Jinbao Lv,Di Lv,Huanming Xu,Hao Shi,Gang Liu,Degui Lin,Yipeng Jin

International journal of molecular sciences 25: PubMed38674064

2024

Comparative Analysis of Olive-Derived Phenolic Compounds' Pro-Melanogenesis Effects on B16F10 Cells and Epidermal Human Melanocytes.

Applications

Unspecified application

Species

Unspecified reactive species

Juhee Cho,Meriem Bejaoui,Kenichi Tominaga,Hiroko Isoda

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-TRP2/DCT antibody

Western blot - Anti-TRP2/DCT antibody (AB74073)

Immunocytochemistry/ Immunofluorescence - Anti-TRP2/DCT antibody (AB74073)

Immunocytochemistry/ Immunofluorescence - Anti-TRP2/DCT antibody (AB74073)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Alternative Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Complementary Products

Primary Antibodies

Primary Antibodies

Secondary Antibodies

Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (73)

Product promise