Anti-TRP2/DCT antibody [EPR21986] (ab221144) is a rabbit monoclonal antibody that is used to detect TRP2/DCT in Western Blot, IP, IHC-P, mIHC. Suitable for Human, Mouse samples.
- Validated for multiplex IHC on the Leica BOND® MAX using Opal reagents
-Using biophysical QC, antibody identity is confirmed at a molecular level for unrivalled batch-batch consistency
-Over 10 publications
Images
![Western blot - Anti-TRP2/DCT antibody [EPR21986] (AB221144), expandable thumbnail](https://content.abcam.com/products/images/trp2-dct-antibody-epr21986-ab221144--western-blot-img61120.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-TRP2/DCT antibody [EPR21986] (AB221144)")
![Immunoprecipitation - Anti-TRP2/DCT antibody [EPR21986] (AB221144), expandable thumbnail](https://content.abcam.com/products/images/trp2-dct-antibody-epr21986-ab221144--immunoprecipitation-img11355.jpg/_jcr_content/renditions/webp-475.webp "Immunoprecipitation - Anti-TRP2/DCT antibody [EPR21986] (AB221144)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRP2/DCT antibody [EPR21986] (AB221144), expandable thumbnail](https://content.abcam.com/products/images/trp2-dct-antibody-epr21986-ab221144--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img118785.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRP2/DCT antibody [EPR21986] (AB221144)")
![Immunoprecipitation - Anti-TRP2/DCT antibody [EPR21986] (AB221144), expandable thumbnail](https://content.abcam.com/products/images/trp2-dct-antibody-epr21986-ab221144--immunoprecipitation-img3817.jpg/_jcr_content/renditions/webp-475.webp "Immunoprecipitation - Anti-TRP2/DCT antibody [EPR21986] (AB221144)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRP2/DCT antibody [EPR21986] (AB221144), expandable thumbnail](https://content.abcam.com/products/images/trp2-dct-antibody-epr21986-ab221144--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img86331.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRP2/DCT antibody [EPR21986] (AB221144)")
Publications
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- Heliyon 9:e217442023Exploring the mechanism of Liuwei Dihuang formula for promoting melanin synthesis in juvenile zebrafish based on network pharmacology and molecular docking.Applications:Unspecified applicationReactive species:Unspecified reactive speciesDandan Wang,Yan Yang,Gulijiayina Hengerjia,Yan DengPubMed 38027573
- Journal of oral pathology & medicine : official publication of the International Association of Oral Pathologists and the American Academy of Oral Pathology 52:1013-10202023Oral melanoacanthoma: Clinicopathological and immunohistochemical features of a case series and a scoping review.Applications:Unspecified applicationReactive species:Unspecified reactive speciesThalita Soares Tavares,Adriana Aparecida Silva da Costa,Felipe da Mata Camargos,Ricardo Santiago Gomez,Cassiano Francisco Weege Nonaka,Pollianna Muniz Alves,Elismauro Francisco Mendonça,Sebastião Silvério Sousa-Neto,Ana Carolina Uchoa Vasconcelos,Sandra Beatriz Chaves Tarquínio,Patrícia Carlos CaldeiraPubMed 37852303
- Disease markers 2022:68326802022Study on the Mechanism of miR-125b-5p Affecting Melanocyte Biological Behavior and Melanogenesis in Vitiligo through Regulation of MITF.Applications:Unspecified applicationReactive species:Unspecified reactive speciesXiaochuan Wang,Yifei Wu,Peng Du,Liangheng Xu,Yuan Chen,Jingzi Li,Xuying Pan,Zhiqiong WangPubMed 36438898
- Molecules (Basel, Switzerland) 27:2022Molecular Analysis of the Melanogenesis Inhibitory Effect of Saponins-Rich Fraction of Leaves Extract.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMyra O Villareal,Thanyanan Chaochaiphat,Rachida Makbal,Chemseddoha Gadhi,Hiroko IsodaPubMed 36235295
- Frontiers in pharmacology 13:9631602022. Extract exhibits pigmentation by melanin biosynthesis and melanosome biogenesis/transfer.Applications:Unspecified applicationReactive species:Unspecified reactive speciesChen Hong,Lili Yang,Yifan Zhang,Yiming Li,Huali WuPubMed 36249817
- Phytotherapy research : PTR 36:4278-42922022Vegetable-derived indole enhances the melanoma-treating efficacy of chemotherapeutics.Applications:Unspecified applicationReactive species:Unspecified reactive speciesBei Bei Zhou,Dan Liu,Jia Cheng Qian,Ren Xiang TanPubMed 35883268
- Frontiers in medicine 9:8126532022Solamargine Alleviated UVB-Induced Inflammation and Melanogenesis in Human Keratinocytes and Melanocytes the p38 MAPK Signaling Pathway, a Promising Agent for Post-inflammatory Hyperpigmentation.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJuemin Zhao,Yanjun Dan,Ziqi Liu,Qianqian Wang,Min Jiang,Chengfeng Zhang,Hamm-Ming Sheu,Chrang-Shi Lin,Leihong XiangPubMed 35770009
- International journal of biological sciences 18:166-1792022Conjugate of ibrutinib with a TLR7 agonist suppresses melanoma progression and enhances antitumor immunity.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSumei Ren,Xiaodong Wang,Guangyi JinPubMed 34975325
- Frontiers in pharmacology 12:7837302021The Inhibitory Effect of Curcumin Derivative J147 on Melanogenesis and Melanosome Transport by Facilitating ERK-Mediated MITF Degradation.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJinpeng Lv,Ying Yang,Bingyi Jia,Siqi Li,Ximei Zhang,Rongyin GaoPubMed 34887767
- Genes & diseases 8:677-6882021SFRP5 inhibits melanin synthesis of melanocytes in vitiligo by suppressing the Wnt/β-catenin signaling.Applications:Unspecified applicationReactive species:Unspecified reactive speciesDao-Pei Zou,Yang-Mei Chen,Ling-Zhao Zhang,Xiao-Hui Yuan,Yu-Jie Zhang,Adelina Inggawati,Pham Thi Kieu Nguyet,Tian-Wen Gao,Jin ChenPubMed 34291139
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IP | WB | IHC-P | mIHC | |
|---|---|---|---|---|
| Human | Tested | Tested | Tested | Tested |
| Mouse | Tested | Tested | Expected | Expected |
IP
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/30 | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Species Human | Dilution info 1/30 | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/1000 | Notes - |
Species Human | Dilution info 1/1000 | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/2000 | Notes Mouse species is recommended based on WB and IP results, we do not guarantee IHC-P for mouse. Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
mIHC
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/3000 | Notes Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
Associated Products
Select an associated product type
1 product for Alternative Product
Target data
Function
Plays a role in melanin biosynthesis (PubMed:33100333). Catalyzes the conversion of L-dopachrome into 5,6-dihydroxyindole-2-carboxylic acid (DHICA).
Alternative names
TYRP2, DCT, L-dopachrome tautomerase, DT, L-dopachrome Delta-isomerase, Tyrosinase-related protein 2, TRP-2, TRP2
Recommended products
Anti-TRP2/DCT antibody [EPR21986] (ab221144) is a rabbit monoclonal antibody that is used to detect TRP2/DCT in Western Blot, IP, IHC-P, mIHC. Suitable for Human, Mouse samples.
- Validated for multiplex IHC on the Leica BOND® MAX using Opal reagents
-Using biophysical QC, antibody identity is confirmed at a molecular level for unrivalled batch-batch consistency
-Over 10 publications
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Clone number
- EPR21986
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Anti-TRP2/DCT antibody [EPR21986] (ab221144) is a rabbit recombinant monoclonal antibody and is validated for use in Western Blot (WB), Immunoprecipitation (IP), Immunohistochemistry (IHC-P), Multiplex IHC (mIHC), in Human, Mouse samples.
What is the molecular weight of TRP2/DCT?
Anti-TRP2/DCT [EPR21986] (ab221144) specifically detects a band for TRP2/DCT (UniProt: P40126) at a molecular weight of 59kDa.
Recommended positive controls
WB: MeWo, SK-MEL-2, SK-MEL-28 and F9 whole cell lysates.IHC-P: Human skin and melanoma tissues.IP: SK-MEL-2 and B16-F0 whole cell lysates.mIHC: Human skin (melanocytes).
Trusted by the scientific community
Anti-TRP2/DCT [EPR21986] (ab221144) was first used in a scientific publication in 2018 and has been cited over 10 times in peer-reviewed journals.
Trial sizes available!
Test your antibody or perform pre-screening before committing to a larger quantity. Sold in 10µl. Discover our selection of trial-size antibodies.Other related products
We have a range of other formats of antibody clone [EPR21986] also available for your convenience:
ab221144, Carrier free - Anti-TRP2/DCT antibody [EPR21986] - BSA and Azide free ab234901
Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
TRP2 also known as DCT or dopachrome tautomerase is a protein involved in melanin synthesis and is part of the tyrosinase-related protein family. It has a molecular weight of approximately 60 kDa. TRP2/DCT is mainly expressed in melanocytes which are cells responsible for pigment production. The protein functions in the melanin biosynthetic pathway converting dopachrome into DHICA contributing to the eumelanin formation which impacts skin and hair color.
- Biological function summary
TRP2/DCT participates in pigmentation and photoprotection processes. It functions in tandem with other enzymes like tyrosinase and TRP1 to form a multiprotein complex important for melanin synthesis. The coordination of these proteins ensures the effective production and regulation of melanin which absorbs ultraviolet radiation and reduces DNA damage in skin cells.
- Pathways
TRP2/DCT plays a role in the melanin biosynthetic pathway and influences the Wnt signaling pathway. The melanin pathway involves enzymes such as tyrosinase which initiates the conversion of tyrosine to L-DOPA and eventually to melanin pigments. The Wnt signaling pathway critical for cellular processes like proliferation and differentiation interacts with melanogenesis through proteins like beta-catenin influencing TRP2/DCT expression and activity.
- Associated diseases and disorders
TRP2/DCT has significant associations with melanoma and vitiligo. In melanoma altered expression of TRP2/DCT can impact tumor cell proliferation and survival often interacting with proteins like MITF an important regulator of melanocyte development. In vitiligo changes in TRP2/DCT expression can disrupt melanin production leading to depigmented patches on the skin. Understanding these interactions and expressions aids in potential therapeutic approaches for these skin conditions.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
6 product images
![Western blot - Anti-TRP2/DCT antibody [EPR21986] (ab221144), expandable thumbnail](https://content.abcam.com/products/images/trp2-dct-antibody-epr21986-ab221144--western-blot-img61120.jpg "Western blot - Anti-TRP2/DCT antibody [EPR21986] (ab221144)")
Western blot - Anti-TRP2/DCT antibody [EPR21986] (ab221144)
Blocking/Dilution buffer: 5% NFDM/TBST.
Exposure time : Lane 1: 5 seconds; Lane 2: 15 seconds; Lane 3: 26 seconds; Lane 4: 3 minutes.
The molecular mass observed is consistent with what has been described in the literature (PMID: 12719423, PMID: 25009701)
All lanes: Western blot - Anti-TRP2/DCT antibody [EPR21986] (ab221144) at 1/1000 dilution
Lane 1: MeWo (human malignant melanoma cell line) whole cell lysate at 20 µg
Lane 2: SK-MEL-2 (human skin malignant melanoma cell line) whole cell lysate at 20 µg
Lane 3: SK-MEL-28 (human malignant melanoma cell line) whole cell lysate at 20 µg
Lane 4: F9 (mouse embryonic testicular cancer cell line) whole cell lysate at 20 µg
SecondaryLane 1: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Lanes 2 - 4: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution
Predicted band size: 59 kDa
Observed band size: 69 kDa, 85 kDa
![Immunoprecipitation - Anti-TRP2/DCT antibody [EPR21986] (ab221144), expandable thumbnail](https://content.abcam.com/products/images/trp2-dct-antibody-epr21986-ab221144--immunoprecipitation-img11355.jpg "Immunoprecipitation - Anti-TRP2/DCT antibody [EPR21986] (ab221144)")
Immunoprecipitation - Anti-TRP2/DCT antibody [EPR21986] (ab221144)
TRP2/DCT was immunoprecipitated from 0.35 mg B16-F0 (mouse melanoma epithelial cell-like cell line) whole cell lysate with ab221144 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab221144 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366), was used for detection at 1/5000 dilution.
Lane 1: B16-F0 whole cell lysate 10 μg (Input).
Lane 2: ab221144 IP in B16-F0 whole cell lysate.
Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of ab221144 in B16-F0 whole cell lysate.
Blocking/Dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 41 seconds.
~85KD band is the glycosylated form (PMID: 12719423)
All lanes: Immunoprecipitation - Anti-TRP2/DCT antibody [EPR21986] (ab221144)
Predicted band size: 59 kDa
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRP2/DCT antibody [EPR21986] (ab221144), expandable thumbnail](https://content.abcam.com/products/images/trp2-dct-antibody-epr21986-ab221144--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img118785.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRP2/DCT antibody [EPR21986] (ab221144)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRP2/DCT antibody [EPR21986] (ab221144)
Immunohistochemical analysis of paraffin-embedded human melanoma tissue labeling TRP2/DCT with ab221144 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Cytoplasmic staining in human melanoma (PMID: 24475287). Counter stained with hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
![Immunoprecipitation - Anti-TRP2/DCT antibody [EPR21986] (ab221144), expandable thumbnail](https://content.abcam.com/products/images/trp2-dct-antibody-epr21986-ab221144--immunoprecipitation-img3817.jpg "Immunoprecipitation - Anti-TRP2/DCT antibody [EPR21986] (ab221144)")
Immunoprecipitation - Anti-TRP2/DCT antibody [EPR21986] (ab221144)
TRP2/DCT was immunoprecipitated from 0.35 mg SK-MEL-2 (human skin malignant melanoma cell line) whole cell lysate with ab221144 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab221144 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366), was used for detection at 1/5000 dilution.
Lane 1: SK-MEL-2 whole cell lysate 10 μg (Input).
Lane 2: ab221144 IP in SK-MEL-2 whole cell lysate.
Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of ab221144 in SK-MEL-2 whole cell lysate.
Blocking/Dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 5 seconds.
All lanes: Immunoprecipitation - Anti-TRP2/DCT antibody [EPR21986] (ab221144)
Predicted band size: 59 kDa
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRP2/DCT antibody [EPR21986] (ab221144), expandable thumbnail](https://content.abcam.com/products/images/trp2-dct-antibody-epr21986-ab221144--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img86331.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRP2/DCT antibody [EPR21986] (ab221144)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRP2/DCT antibody [EPR21986] (ab221144)
Immunohistochemical analysis of paraffin-embedded human skin tissue labeling TRP2/DCT with ab221144 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Cytoplasmic staining in melanocytes of human skin (PMID:12535195). Counter stained with hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
![Multiplex immunohistochemistry - Anti-TRP2/DCT antibody [EPR21986] (ab221144), expandable thumbnail](https://content.abcam.com/products/images/trp2-dct-antibody-epr21986-ab221144--multiplex-immunohistochemistry-img422545.jpg "Multiplex immunohistochemistry - Anti-TRP2/DCT antibody [EPR21986] (ab221144)")
Multiplex immunohistochemistry - Anti-TRP2/DCT antibody [EPR21986] (ab221144)
Panel A: merged staining of anti-Desmoglein 1/DSG1 (gray; Opal™690), anti-Langerin (green; Opal™520) and anti-TRP2/DCT (red; Opal™570) on human skin.
Panel B: anti-TRP2/DCT stained on melanocytes.
Panel C: anti-Langerin stained on Langerhans cells.
Panel D: anti-Desmoglein 1/DSG1 stained on epidermis.
The section was incubated in three rounds of staining: in the order of Anti-Desmoglein 1/DSG1 antibody [EPR20382] - BSA and Azide free ab236259, Anti-Langerin antibody [EPR24685-12] ab283686, and ab221144 for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument with an Opal™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope.
![Multiplex immunohistochemistry - Anti-TRP2/DCT antibody [EPR21986] (ab221144), expandable thumbnail](https://content.abcam.com/products/images/trp2-dct-antibody-epr21986-ab221144--multiplex-immunohistochemistry-img422545.jpg/_jcr_content/renditions/webp-475.webp "Multiplex immunohistochemistry - Anti-TRP2/DCT antibody [EPR21986] (ab221144)")
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