Rabbit Recombinant Monoclonal TRP2/DCT antibody. Carrier free. Suitable for IP, WB, IHC-P, mIHC and reacts with Mouse, Human samples. Cited in 1 publication.
Images
![Immunoprecipitation - Anti-TRP2/DCT antibody [EPR21986] - BSA and Azide free (AB234901), expandable thumbnail](https://content.abcam.com/products/images/trp2-dct-antibody-epr21986-bsa-and-azide-free-ab234901--immunoprecipitation-img11355.jpg/_jcr_content/renditions/webp-475.webp "Immunoprecipitation - Anti-TRP2/DCT antibody [EPR21986] - BSA and Azide free (AB234901)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRP2/DCT antibody [EPR21986] - BSA and Azide free (AB234901), expandable thumbnail](https://content.abcam.com/products/images/trp2-dct-antibody-epr21986-bsa-and-azide-free-ab234901--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img118785.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRP2/DCT antibody [EPR21986] - BSA and Azide free (AB234901)")
![Immunoprecipitation - Anti-TRP2/DCT antibody [EPR21986] - BSA and Azide free (AB234901), expandable thumbnail](https://content.abcam.com/products/images/trp2-dct-antibody-epr21986-bsa-and-azide-free-ab234901--immunoprecipitation-img3817.jpg/_jcr_content/renditions/webp-475.webp "Immunoprecipitation - Anti-TRP2/DCT antibody [EPR21986] - BSA and Azide free (AB234901)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRP2/DCT antibody [EPR21986] - BSA and Azide free (AB234901), expandable thumbnail](https://content.abcam.com/products/images/trp2-dct-antibody-epr21986-bsa-and-azide-free-ab234901--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img86331.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRP2/DCT antibody [EPR21986] - BSA and Azide free (AB234901)")
![Multiplex immunohistochemistry - Anti-TRP2/DCT antibody [EPR21986] - BSA and Azide free (AB234901), expandable thumbnail](https://content.abcam.com/products/images/trp2-dct-antibody-epr21986-bsa-and-azide-free-ab234901--multiplex-immunohistochemistry-img422545.jpg/_jcr_content/renditions/webp-475.webp "Multiplex immunohistochemistry - Anti-TRP2/DCT antibody [EPR21986] - BSA and Azide free (AB234901)")
Publications
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- Iranian journal of public health 51:2706-27162023Peripheral Blood of Vitiligo Patients-Derived Exosomal MiR-21-5p Inhibits Melanocytes Melanogenesis via Targeting SATB1.Applications:Unspecified applicationReactive species:Unspecified reactive speciesChange Zhang,Wu Guo,Sheng Wang,Yuzi Di,Dengting WuPubMed 36742227
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IP | WB | IHC-P | mIHC | |
|---|---|---|---|---|
| Human | Tested | Tested | Tested | Tested |
| Mouse | Tested | Expected | Expected | Expected |
IP
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes - |
Species Human | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes Mouse species is recommended based on WB and IP results, we do not guarantee IHC-P for mouse. Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
mIHC
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
Associated Products
Select an associated product type
2 products for Alternative Product
Target data
Function
Plays a role in melanin biosynthesis (PubMed:33100333). Catalyzes the conversion of L-dopachrome into 5,6-dihydroxyindole-2-carboxylic acid (DHICA).
Alternative names
TYRP2, DCT, L-dopachrome tautomerase, DT, L-dopachrome Delta-isomerase, Tyrosinase-related protein 2, TRP-2, TRP2
Recommended products
Rabbit Recombinant Monoclonal TRP2/DCT antibody. Carrier free. Suitable for IP, WB, IHC-P, mIHC and reacts with Mouse, Human samples. Cited in 1 publication.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
- Yes
- Clone number
- EPR21986
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- Do Not Freeze
Notes
ab234901 is the carrier-free version of Anti-TRP2/DCT antibody [EPR21986] ab221144.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
TRP2 also known as DCT or dopachrome tautomerase is a protein involved in melanin synthesis and is part of the tyrosinase-related protein family. It has a molecular weight of approximately 60 kDa. TRP2/DCT is mainly expressed in melanocytes which are cells responsible for pigment production. The protein functions in the melanin biosynthetic pathway converting dopachrome into DHICA contributing to the eumelanin formation which impacts skin and hair color.
- Biological function summary
TRP2/DCT participates in pigmentation and photoprotection processes. It functions in tandem with other enzymes like tyrosinase and TRP1 to form a multiprotein complex important for melanin synthesis. The coordination of these proteins ensures the effective production and regulation of melanin which absorbs ultraviolet radiation and reduces DNA damage in skin cells.
- Pathways
TRP2/DCT plays a role in the melanin biosynthetic pathway and influences the Wnt signaling pathway. The melanin pathway involves enzymes such as tyrosinase which initiates the conversion of tyrosine to L-DOPA and eventually to melanin pigments. The Wnt signaling pathway critical for cellular processes like proliferation and differentiation interacts with melanogenesis through proteins like beta-catenin influencing TRP2/DCT expression and activity.
- Associated diseases and disorders
TRP2/DCT has significant associations with melanoma and vitiligo. In melanoma altered expression of TRP2/DCT can impact tumor cell proliferation and survival often interacting with proteins like MITF an important regulator of melanocyte development. In vitiligo changes in TRP2/DCT expression can disrupt melanin production leading to depigmented patches on the skin. Understanding these interactions and expressions aids in potential therapeutic approaches for these skin conditions.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
5 product images
![Immunoprecipitation - Anti-TRP2/DCT antibody [EPR21986] - BSA and Azide free (ab234901), expandable thumbnail](https://content.abcam.com/products/images/trp2-dct-antibody-epr21986-bsa-and-azide-free-ab234901--immunoprecipitation-img11355.jpg "Immunoprecipitation - Anti-TRP2/DCT antibody [EPR21986] - BSA and Azide free (ab234901)")
Immunoprecipitation - Anti-TRP2/DCT antibody [EPR21986] - BSA and Azide free (ab234901)
TRP2/DCT was immunoprecipitated from 0.35 mg B16-F0 (mouse melanoma epithelial cell-like cell line) whole cell lysate with Anti-TRP2/DCT antibody [EPR21986] ab221144 at 1/30 dilution. Western blot was performed from the immunoprecipitate using Anti-TRP2/DCT antibody [EPR21986] ab221144 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366), was used for detection at 1/5000 dilution.
Lane 1: B16-F0 whole cell lysate 10 μg (Input).
Lane 2: Anti-TRP2/DCT antibody [EPR21986] ab221144 IP in B16-F0 whole cell lysate.
Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of Anti-TRP2/DCT antibody [EPR21986] ab221144 in B16-F0 whole cell lysate.
Blocking/Dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 41 seconds.
~85KD band is the glycosylated form (PMID: 12719423)
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-TRP2/DCT antibody [EPR21986] ab221144).
All lanes: Immunoprecipitation - Anti-TRP2/DCT antibody [EPR21986] (Anti-TRP2/DCT antibody [EPR21986] ab221144)
Predicted band size: 59 kDa
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRP2/DCT antibody [EPR21986] - BSA and Azide free (ab234901), expandable thumbnail](https://content.abcam.com/products/images/trp2-dct-antibody-epr21986-bsa-and-azide-free-ab234901--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img118785.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRP2/DCT antibody [EPR21986] - BSA and Azide free (ab234901)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRP2/DCT antibody [EPR21986] - BSA and Azide free (ab234901)
Immunohistochemical analysis of paraffin-embedded human melanoma tissue labeling TRP2/DCT with Anti-TRP2/DCT antibody [EPR21986] ab221144 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Cytoplasmic staining in human melanoma (PMID: 24475287). Counter stained with hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-TRP2/DCT antibody [EPR21986] ab221144).
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
![Immunoprecipitation - Anti-TRP2/DCT antibody [EPR21986] - BSA and Azide free (ab234901), expandable thumbnail](https://content.abcam.com/products/images/trp2-dct-antibody-epr21986-bsa-and-azide-free-ab234901--immunoprecipitation-img3817.jpg "Immunoprecipitation - Anti-TRP2/DCT antibody [EPR21986] - BSA and Azide free (ab234901)")
Immunoprecipitation - Anti-TRP2/DCT antibody [EPR21986] - BSA and Azide free (ab234901)
TRP2/DCT was immunoprecipitated from 0.35 mg SK-MEL-2 (human skin malignant melanoma cell line) whole cell lysate with Anti-TRP2/DCT antibody [EPR21986] ab221144 at 1/30 dilution. Western blot was performed from the immunoprecipitate using Anti-TRP2/DCT antibody [EPR21986] ab221144 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366), was used for detection at 1/5000 dilution.
Lane 1: SK-MEL-2 whole cell lysate 10 μg (Input).
Lane 2: Anti-TRP2/DCT antibody [EPR21986] ab221144 IP in SK-MEL-2 whole cell lysate.
Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of Anti-TRP2/DCT antibody [EPR21986] ab221144 in SK-MEL-2 whole cell lysate.
Blocking/Dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 5 seconds.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-TRP2/DCT antibody [EPR21986] ab221144).
All lanes: Immunoprecipitation - Anti-TRP2/DCT antibody [EPR21986] (Anti-TRP2/DCT antibody [EPR21986] ab221144)
Predicted band size: 59 kDa
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRP2/DCT antibody [EPR21986] - BSA and Azide free (ab234901), expandable thumbnail](https://content.abcam.com/products/images/trp2-dct-antibody-epr21986-bsa-and-azide-free-ab234901--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img86331.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRP2/DCT antibody [EPR21986] - BSA and Azide free (ab234901)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRP2/DCT antibody [EPR21986] - BSA and Azide free (ab234901)
Immunohistochemical analysis of paraffin-embedded human skin tissue labeling TRP2/DCT with Anti-TRP2/DCT antibody [EPR21986] ab221144 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Cytoplasmic staining in melanocytes of human skin (PMID:12535195). Counter stained with hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-TRP2/DCT antibody [EPR21986] ab221144).
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
![Multiplex immunohistochemistry - Anti-TRP2/DCT antibody [EPR21986] - BSA and Azide free (ab234901), expandable thumbnail](https://content.abcam.com/products/images/trp2-dct-antibody-epr21986-bsa-and-azide-free-ab234901--multiplex-immunohistochemistry-img422545.jpg "Multiplex immunohistochemistry - Anti-TRP2/DCT antibody [EPR21986] - BSA and Azide free (ab234901)")
Multiplex immunohistochemistry - Anti-TRP2/DCT antibody [EPR21986] - BSA and Azide free (ab234901)
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-TRP2/DCT antibody [EPR21986] ab221144).
Panel A: merged staining of anti-Desmoglein 1/DSG1 (gray; Opal™690), anti-Langerin (green; Opal™520) and anti-TRP2/DCT (red; Opal™570) on human skin.
Panel B: anti-TRP2/DCT stained on melanocytes.
Panel C: anti-Langerin stained on Langerhans cells.
Panel D: anti-Desmoglein 1/DSG1 stained on epidermis.
The section was incubated in three rounds of staining: in the order of Anti-Desmoglein 1/DSG1 antibody [EPR20382] - BSA and Azide free ab236259, Anti-Langerin antibody [EPR24685-12] ab283686, and Anti-TRP2/DCT antibody [EPR21986] ab221144 for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument with an Opal™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope.
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