Anti-TRPA1/TSA antibody [EPR26211-139] - BSA and Azide free

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRPA1/TSA antibody [EPR26211-139] - BSA and Azide free (AB320716)

This data was developed using ab320715, the same antibody clone in a different buffer formulation. Immunohistochemical analysis of paraffin-embedded (A) HEK-293T (human epithelial cell line from embryonic kidney) transfected with a TRPA1 expression vector containing a Myc-His tag. (B) HEK-293T transfected with empty vector containing a Myc-His tag. tissue labeling TRPA1/TSA with ab320715 at 1/2000 (0.25 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Positive staining on (A) HEK-293T (human epithelial cell line from embryonic kidney) transfected with a TRPA1 expression vector containing a Myc-His tag, negative staining on (B) HEK-293T transfected with empty vector containing a Myc-His tag. The section was incubated with ab320715 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRPA1/TSA antibody [EPR26211-139] - BSA and Azide free (AB320716)

This data was developed using ab320715, the same antibody clone in a different buffer formulation. Immunohistochemical analysis of paraffin-embedded Human liver tissue labeling TRPA1/TSA with ab320715 at 1/50 (9.98 ug/ml) dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP polymer) (ab214880). Negative control : no staining on human liver. The section was incubated with ab320715 at 4°C overnight. Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP polymer) (ab214880). Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0)

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRPA1/TSA antibody [EPR26211-139] - BSA and Azide free (AB320716)

This data was developed using ab320715, the same antibody clone in a different buffer formulation. Immunohistochemical analysis of paraffin-embedded Human lung tissue labeling TRPA1/TSA with ab320715 at 1/50 (9.98 ug/ml) dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP polymer) (ab214880). Negative control : no staining on human lung. The section was incubated with ab320715 at 4°C overnight. Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP polymer) (ab214880). Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0)

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRPA1/TSA antibody [EPR26211-139] - BSA and Azide free (AB320716)

This data was developed using ab320715, the same antibody clone in a different buffer formulation. Immunohistochemical analysis of paraffin-embedded Human dorsal root ganglion (DRG) tissue labeling TRPA1/TSA with ab320715 at 1/50 (9.98 ug/ml) dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP polymer) (ab214880). Positive staining on human dorsal root ganglion (DRG). The section was incubated with ab320715 at 4°C overnight. Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP polymer) (ab214880). Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0)

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRPA1/TSA antibody [EPR26211-139] - BSA and Azide free (AB320716)

This data was developed using ab320715, the same antibody clone in a different buffer formulation. Immunohistochemical analysis of paraffin-embedded Muscularis of human colon tissue labeling TRPA1/TSA with ab320715 at 1/50 (9.98 ug/ml) dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP polymer) (ab214880). Positive staining on myenteric ganglia of human colon (PMID : 28478727). The section was incubated with ab320715 at 4°C overnight. Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP polymer) (ab214880). Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0)

• WB

Supplier Data

Western blot - Anti-TRPA1/TSA antibody [EPR26211-139] - BSA and Azide free (AB320716)

This data was developed using ab320715, the same antibody clone in a different buffer formulation. Blocking and diluting buffer and concentration : 5% NFDM/TBST. Low expression : HEK-293, T-47D, Jurkat. The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID : 24008932). The identity of the band higher than 250 kDa (in lanes 2-3) is unknown.  The identity of the lower MW bands at approximately 15 kDa (in lane 4) and 45 kDa (in lane 5) are unknown. In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control  (ab181602) staining at 1/200000 dilution.

All lanes:

Western blot - Anti-TRPA1/TSA antibody [EPR26211-139] (<a href='/en-us/products/primary-antibodies/trpa1-tsa-antibody-epr26211-139-ab320715'>ab320715</a>) at 1/1000 dilution

Lane 1:

U-2 OS (human bone osteosarcoma epithelial cell) whole cell lysate at 50 µg

Lane 2:

MRC-5 (human lung fibroblast) whole cell lysate at 50 µg

Lane 3:

WI-8 (human fetal lung fibroblast) whole cell lysate at 50 µg

Lane 4:

HEK-293 (human embryonic kidney epithelial cell) whole cell lysate at 50 µg

Lane 5:

T-47D (human ductal breast epithelial tumor epithelial cell) whole cell lysate at 50 µg

Lane 6:

Jurkat (human T cell leukemia T lymphocyte from peripheral blood) whole cell lysate at 50 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 130 kDa,140 kDa,36 kDa

false

Exposure time: 81s