AB51255

Anti-TRPC1 antibody [EP1417Y]

Name: Anti-TRPC1 antibody [EP1417Y] (ab51255) | Abcam
Brand: Abcam Limited
SKU: AB51255
Price: 330 USD
Availability: InStock
Rating: 5 (1 reviews)

RabMAb
Recombinant
What is this?

(1 Review)

(11 Publications)

Rabbit Recombinant Monoclonal TRPC1 antibody. Suitable for WB and reacts with Human samples. Cited in 11 publications.

View Alternative Names

TRP1, TRPC1, Short transient receptor potential channel 1, TrpC1, Transient receptor protein 1, TRP-1

2 Images

Unknown

Western blot - Anti-TRPC1 antibody [EP1417Y] (AB51255)

All lanes:

Western blot - Anti-TRPC1 antibody [EP1417Y] (ab51255) at 1/10000 dilution

All lanes:

HepG2 cell lysate at 10 µg

Secondary

All lanes:

Goat anti-Rabbit HRP labeled at 1/2000 dilution

Predicted band size: 91 kDa

Observed band size: 120 kDa

false

AbReview20252****

Western blot - Anti-TRPC1 antibody [EP1417Y] (AB51255)

Blocking Step : 5% Milk for 16 hours

All lanes:

Western blot - Anti-TRPC1 antibody [EP1417Y] (ab51255) at 1/500 dilution

All lanes:

Rat artery tissue lysate at 50 µg

Secondary

All lanes:

An HRP-conjugated Goat anti-rabbit polyclonal at 1/10000 dilution

Predicted band size: 91 kDa

Observed band size: ~100 kDa

true

Exposure time: 5min

This image is courtesy of an anonymous Abreview

Carrier free

Anti-TRPC1 antibody [EP1417Y] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EP1417Y

Isotype

IgG

Carrier free

Reacts with

Human

Applications

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHC" : {"fullname" : "Immunohistochemistry", "shortname":"IHC"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHC-species-checked": "notRecommended", "IHC-species-dilution-info": "", "IHC-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/10000", "WB-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "IHC-species-checked": "notRecommended", "IHC-species-dilution-info": "", "IHC-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Product details

Species reactivity
Mouse: We have preliminary internal testing data to indicate this antibody may not react with this species.
Please contact us for more information.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: 50% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

TRPC1 also known as the Transient Receptor Potential Channel 1 is a protein that forms ion channels in cell membranes facilitating the movement of calcium ions into cells. TRPC1 has a molecular mass of approximately 89 kDa. It is mainly expressed in the central nervous system such as the brain as well as in tissues like the heart lungs and skeletal muscles. TRPC1 operates as a non-selective cation channel that responds to a variety of stimuli including mechanical thermal and chemical signals.

Biological function summary

TRPC1 plays roles in various cellular functions including cell growth muscle contraction and ion homeostasis. TRPC1 often associates with other TRP channel proteins forming heteromeric complexes that modify its channel properties. Through these complexes TRPC1 contributes to regulating intracellular calcium levels which are important for numerous physiological processes. Its role in maintaining calcium balance highlights its importance in cellular signalling and functional regulation in many cell types.

Pathways

Researchers identify TRPC1 as an essential component of the calcium signalling pathway influencing cellular activities like proliferation and apoptosis. TRPC1 also integrates within the phosphoinositide signalling pathway where it interacts with proteins such as STIM1 and Orai1. These interactions facilitate the entry of calcium ions into the cells important in conditions of calcium store depletion and cellular signalling cascades.

TRPC1 is implicated in cardiovascular diseases and neurodegenerative disorders. Dysregulation of TRPC1 activity leads to altered calcium homoeostasis contributing to the development and progression of conditions like hypertension and Alzheimer's disease. In Alzheimer's interactions between TRPC1 and amyloid precursor protein (APP) may influence neuronal function and survival showcasing its potential as a therapeutic target for research and drug development in these life-impacting conditions.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Forms a receptor-activated non-selective calcium permeant cation channel. Probably is operated by a phosphatidylinositol second messenger system activated by receptor tyrosine kinases or G-protein coupled receptors. Also activated by intracellular calcium store depletion.. Isoform Short. Forms a receptor-activated non-selective calcium permeant cation channel. Also activated by intracellular calcium store depletion.

See full target information TRPC1

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Publications (11)

Recent publications for all applications. Explore the full list and refine your search

Journal of lipid research 66:100718 PubMed39631563

2024

Porcine transient receptor potential channel 1 promotes adipogenesis and lipid deposition.

Applications

Unspecified application

Species

Unspecified reactive species

Yu Fu,Xin Hao,Jingru Nie,Peng Shang,Xinxing Dong,Bo Zhang,Dawei Yan,Hao Zhang

Journal of ginseng research 47:89-96 PubMed36644379

2023

Polysaccharides from promote intestinal epithelial cell migration through affecting the Ca related regulators.

Applications

Unspecified application

Species

Unspecified reactive species

Huibin Zhu,Jianhong Cao,Xinyi Liang,Meng Luo,Anrong Wang,Ling Hu,Ruliu Li

Aging cell 19:e13209 PubMed32815315

2020

Tau-induced upregulation of C/EBPβ-TRPC1-SOCE signaling aggravates tauopathies: A vicious cycle in Alzheimer neurodegeneration.

Applications

Unspecified application

Species

Unspecified reactive species

Jinwang Ye,Ying Yin,Yaling Yin,Huaqiu Zhang,Huali Wan,Lu Wang,Yue Zuo,Di Gao,Mengzhu Li,Jun Li,Yanchao Liu,Dan Ke,Jian-Zhi Wang

Molecular medicine reports 22:2887-2895 PubMed32700746

2020

Effect of selective inhibition or activation of PGE2 EP1 receptor on glomerulosclerosis.

Applications

Unspecified application

Species

Unspecified reactive species

Xu Chen,Jun Yin,Yuyin Xu,Zhi Qiu,Jing Liu,Xiaolan Chen

Medicine 96:e8166 PubMed29068985

2017

Effects of TRPC1 on epithelial mesenchymal transition in human airway in chronic obstructive pulmonary disease.

Applications

Unspecified application

Species

Unspecified reactive species

Feng Xu,Xiao-Chun Liu,Li Li,Chao-Nan Ma,Ya-Jun Zhang

Arthritis research & therapy 19:27 PubMed28183357

2017

Proangiogenic effects of soluble α-Klotho on systemic sclerosis dermal microvascular endothelial cells.

Applications

Unspecified application

Species

Unspecified reactive species

Celestina Mazzotta,Mirko Manetti,Irene Rosa,Eloisa Romano,Jelena Blagojevic,Silvia Bellando-Randone,Cosimo Bruni,Gemma Lepri,Serena Guiducci,Lidia Ibba-Manneschi,Marco Matucci-Cerinic

The Journal of biological chemistry 287:16146-57 PubMed22451676

2012

TRPC1 protein channel is major regulator of epidermal growth factor receptor signaling.

Applications

Species

Human

Nicolas Tajeddine,Philippe Gailly

American journal of physiology. Cell physiology 302:C77-87 PubMed21940663

2011

Enhanced store-operated Ca²+ entry and TRPC channel expression in pulmonary arteries of monocrotaline-induced pulmonary hypertensive rats.

Applications

Unspecified application

Species

Unspecified reactive species

Xiao-Ru Liu,Ming-Fang Zhang,Na Yang,Qing Liu,Rui-Xing Wang,Yuan-Ning Cao,Xiao-Ru Yang,James S K Sham,Mo-Jun Lin

American journal of physiology. Cell physiology 297:C1103-12 PubMed19675303

2009

Orai1, a critical component of store-operated Ca2+ entry, is functionally associated with Na+/Ca2+ exchanger and plasma membrane Ca2+ pump in proliferating human arterial myocytes.

Applications

Unspecified application

Species

Unspecified reactive species

Sergey G Baryshnikov,Maria V Pulina,Alessandra Zulian,Cristina I Linde,Vera A Golovina

Cell calcium 46:73-84 PubMed19523685

2009

Reduction in TRPC4 expression specifically attenuates G-protein coupled receptor-stimulated increases in intracellular calcium in human myometrial cells.

Applications

Unspecified application

Species

Unspecified reactive species

Aida Ulloa,Albert L Gonzales,Miao Zhong,Yoon-Sun Kim,Jeremy Cantlon,Colin Clay,Chun-Ying Ku,Scott Earley,Barbara M Sanborn

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-TRPC1 antibody [EP1417Y]

Western blot - Anti-TRPC1 antibody [EP1417Y] (AB51255)

Western blot - Anti-TRPC1 antibody [EP1417Y] (AB51255)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (11)

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