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AB251109

Anti-TRPC1 antibody [EPR1417(2)] - BSA and Azide free

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(1 Publication)

Rabbit Recombinant Monoclonal TRPC1 antibody. Carrier free. Suitable for WB and reacts with Mouse, Human, Rat samples. Cited in 1 publication.

View Alternative Names

TRP1, TRPC1, Short transient receptor potential channel 1, TrpC1, Transient receptor protein 1, TRP-1

2 Images
Western blot - Anti-TRPC1 antibody [EPR1417(2)] - BSA and Azide free (AB251109)
  • WB

Supplier Data

Western blot - Anti-TRPC1 antibody [EPR1417(2)] - BSA and Azide free (AB251109)

This data was developed using ab192031, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-TRPC1 antibody [EPR1417(2)] (<a href='/en-us/products/primary-antibodies/trpc1-antibody-epr14172-ab192031'>ab192031</a>) at 1/10000 dilution

Lane 1:

SH-SY5Y lysate at 20 µg

Lane 2:

HepG2 lysate at 20 µg

Lane 3:

Caco-2 lysate at 20 µg

Lane 4:

Human testis lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 91 kDa

Observed band size: 120 kDa

false

Western blot - Anti-TRPC1 antibody [EPR1417(2)] - BSA and Azide free (AB251109)
  • WB

Supplier Data

Western blot - Anti-TRPC1 antibody [EPR1417(2)] - BSA and Azide free (AB251109)

This data was developed using ab192031, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-TRPC1 antibody [EPR1417(2)] (<a href='/en-us/products/primary-antibodies/trpc1-antibody-epr14172-ab192031'>ab192031</a>) at 1/1000 dilution

Lane 1:

Mouse brain lysate at 10 µg

Lane 2:

Rat brain lysate at 10 µg

Lane 3:

PC-12 lysate at 10 µg

Lane 4:

NIH3T3 lysate at 10 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 91 kDa

Observed band size: 120 kDa

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR1417(2)

Isotype

IgG

Carrier free

Yes

Reacts with

Mouse, Rat, Human

Applications

WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>" } } }
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Product details

ab251109 is the carrier-free version of ab192031.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

TRPC1 also known as the Transient Receptor Potential Channel 1 is a protein that forms ion channels in cell membranes facilitating the movement of calcium ions into cells. TRPC1 has a molecular mass of approximately 89 kDa. It is mainly expressed in the central nervous system such as the brain as well as in tissues like the heart lungs and skeletal muscles. TRPC1 operates as a non-selective cation channel that responds to a variety of stimuli including mechanical thermal and chemical signals.
Biological function summary

TRPC1 plays roles in various cellular functions including cell growth muscle contraction and ion homeostasis. TRPC1 often associates with other TRP channel proteins forming heteromeric complexes that modify its channel properties. Through these complexes TRPC1 contributes to regulating intracellular calcium levels which are important for numerous physiological processes. Its role in maintaining calcium balance highlights its importance in cellular signalling and functional regulation in many cell types.

Pathways

Researchers identify TRPC1 as an essential component of the calcium signalling pathway influencing cellular activities like proliferation and apoptosis. TRPC1 also integrates within the phosphoinositide signalling pathway where it interacts with proteins such as STIM1 and Orai1. These interactions facilitate the entry of calcium ions into the cells important in conditions of calcium store depletion and cellular signalling cascades.

TRPC1 is implicated in cardiovascular diseases and neurodegenerative disorders. Dysregulation of TRPC1 activity leads to altered calcium homoeostasis contributing to the development and progression of conditions like hypertension and Alzheimer's disease. In Alzheimer's interactions between TRPC1 and amyloid precursor protein (APP) may influence neuronal function and survival showcasing its potential as a therapeutic target for research and drug development in these life-impacting conditions.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Forms a receptor-activated non-selective calcium permeant cation channel. Probably is operated by a phosphatidylinositol second messenger system activated by receptor tyrosine kinases or G-protein coupled receptors. Also activated by intracellular calcium store depletion.. Isoform Short. Forms a receptor-activated non-selective calcium permeant cation channel. Also activated by intracellular calcium store depletion.
See full target information TRPC1
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Chinese medical journal 137:1105-1114 PubMed37640670

2023

Effects of COL1A1 and SYTL2 on inflammatory cell infiltration and poor extracellular matrix remodeling of the vascular wall in thoracic aortic aneurysm.

Applications

Unspecified application

Species

Unspecified reactive species

Xinsheng Xie,Ye Yuan,Yulong Huang,Xiang Hong,Shichai Hong,Gang Chen,Yihui Chen,Yue Lin,Weifeng Lu,Weiguo Fu,Lixin Wang
View all publications
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Product promise

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For full details, please see our Terms & Conditions

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