AB300143

Anti-TRPC3 antibody [EPR25056-56] (BSA and Azide free)

BOND RX™ Validated
RabMAb
Recombinant
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Rabbit Recombinant Monoclonal TRPC3 antibody. Carrier free. Suitable for IHC-P, WB and reacts with Transfected cell line, Mouse, Rat samples.

View Alternative Names

Trp3, Trrp3, Trpc3, Short transient receptor potential channel 3, TrpC3, Receptor-activated cation channel TRP3, Transient receptor protein 3, Trp-related protein 3, TRP-3, mTrp3

7 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRPC3 antibody [EPR25056-56] (BSA and Azide free) (AB300143)

IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRPC3 antibody [EPR25056-56] (BSA and Azide free) (AB300143)

This data was developed using ab300142, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded (A) HEK-293T cells transfected with a TRPC3 expression vector containing a his tag and (B) HEK-293T cells transfected with empty vector containing a his tag. tissue labeling TRPC3 with ab300142 at 1/500 dilution, followed by a ready to use LeicaDS9800 (Bond^™ Polymer Refine Detection).

Cytoplasmic staining on (A) HEK-293T transfected with a TRPC3 expression vector; nearly no staining on (B) HEK-293T transfected with empty vector.

The section was incubated with ab300142 for 30 mins at room temperature.

The immunostaining was performed on a Leica Biosystems BOND^® RX instrument.

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond^™ Polymer Refine Detection).

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRPC3 antibody [EPR25056-56] (BSA and Azide free) (AB300143)

This data was developed using ab300142, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded mouse liver tissue labeling TRPC3 with ab300142 at 1/100 dilution.

Negative control : no staining on mouse liver.

The section was incubated with ab300142 for 30 mins at room temperature.

The immunostaining was performed on a Leica Biosystems BOND^® RX instrument.

Counterstained with Hematoxylin.

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRPC3 antibody [EPR25056-56] (BSA and Azide free) (AB300143)

This data was developed using ab300142, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue labeling TRPC3 with ab300142 at 1/100 dilution.

Positive staining on mouse cerebellum.

The section was incubated with ab300142 for 30 mins at room temperature.

The immunostaining was performed on a Leica Biosystems BOND^® RX instrument

Counterstained with Hematoxylin.

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRPC3 antibody [EPR25056-56] (BSA and Azide free) (AB300143)

This data was developed using ab300142, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded rat cerebellum tissue labeling TRPC3 with ab300142 at 1/100 dilution, followed by a ready to use LeicaDS9800 (Bond^™ Polymer Refine Detection).

Positive staining on rat cerebellum.

The section was incubated with ab300142 for 30 mins at room temperature.

The immunostaining was performed on a Leica Biosystems BOND^® RX instrument.

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond^™ Polymer Refine Detection).

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

Lab

Western blot - Anti-TRPC3 antibody [EPR25056-56] (BSA and Azide free) (AB300143)

This data was developed using ab300142, the same antibody clone in a different buffer formulation.

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining.

The molecular weight is consistent to literature (PMID : 15834157).

Low express tissue : lung (PMID : 21931279).

All lanes:

Western blot - Anti-TRPC3 antibody [EPR25056-56] (<a href='/en-us/products/primary-antibodies/trpc3-antibody-epr25056-56-ab300142'>ab300142</a>) at 1/1000 dilution

Lane 1:

Mouse cerebellum tissue lysate at 20 µg

Lane 2:

Mouse brain tissue lysate at 20 µg

Lane 3:

Mouse lung tissue lysate at 20 µg

Lane 4:

Rat cerebellum tissue lysate at 20 µg

Lane 5:

Rat brain tissue lysate at 20 µg

Lane 6:

Rat lung tissue lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 105 kDa

true

Exposure time: 3min

Lab

Western blot - Anti-TRPC3 antibody [EPR25056-56] (BSA and Azide free) (AB300143)

This data was developed using ab300142, the same antibody clone in a different buffer formulation.

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining.

All lanes:

Western blot - Anti-TRPC3 antibody [EPR25056-56] (<a href='/en-us/products/primary-antibodies/trpc3-antibody-epr25056-56-ab300142'>ab300142</a>) at 1/1000 dilution

Lane 1:

HEK-293 transfected with mouse TRPC3 (WT) expression vector containing a myc-His-tag®, whole cell lysate at 20 µg

Lane 2:

HEK-293 transfected with mouse TRPC7 (WT) expression vector containing a myc-His-tag®, whole cell lysate at 20 µg

Lane 3:

HEK-293 transfected with mouse TRPC6 (WT) expression vector containing a myc-His-tag®, whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Observed band size: 105 kDa

false

Exposure time: 6s

IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRPC3 antibody [EPR25056-56] (BSA and Azide free) (AB300143)

Immunohistochemical analysis of paraffin-embedded Panel A HEK-293T cells labeling TRPC3 with ab300142 at 1/500 dilution followed by a ready to use LeicaDS9800 (BOND™ Polymer Refine Detection). Cytoplasmic staining on (A) HEK-293T transfected with a TRPC3 expression vector; nearly no staining on (B) HEK-293T transfected with empty vector. The section was incubated with ab300142 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin. Secondary antibody only control : PBS was used instead of primary antibody followed by ready to use secondary antibody LeicaDS9800 (BOND™ Polymer Refine Detection). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

Unconjugated

Anti-TRPC3 antibody [EPR25056-56]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR25056-56

Isotype

IgG

Carrier free

Yes

Reacts with

Human, Mouse, Rat

Applications

IHC-P, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCFr" : {"fullname" : "Immunohistochemistry (Frozen sections)", "shortname":"IHC-Fr"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCFr-species-checked": "notRecommended", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "" }, "Mouse": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCFr-species-checked": "notRecommended", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "" }, "Rat": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCFr-species-checked": "notRecommended", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "" }, "Transfected cell line": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCFr-species-checked": "notRecommended", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "" } } }

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Product details

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Compatibility
This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Aliquoting information

Upon delivery aliquot

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The TRPC3 protein also known as transient receptor potential cation channel subfamily C member 3 is a fundamental component of the plasma membrane and participates in forming a non-selective cation channel. With a molecular mass of approximately 97 kDa TRPC3 channels are widely expressed in the brain heart and vascular smooth muscle. These channels mediate calcium and to a less degree sodium influx when activated facilitating various cellular responses. Additionally TRPC3 channels can interact with other proteins within the same family shaping the channel's functional properties.

Biological function summary

Transient receptor potential channels like TRPC3 play important roles in cellular calcium signaling with direct implications in neuronal cardiac and smooth muscle functions. TRPC3 proteins can assemble into heteromultimeric complexes with other TRPC family members often modulating their biophysical characteristics and responsiveness to stimuli. In neurons TRPC3 channels contribute to synaptic transmission and plasticity by influencing intracellular calcium levels. In smooth muscle cells TRPC3 activity influences contraction and relaxation processes affected by calcium flux regulation.

Pathways

Inositol triphosphate (IP3) and phospholipase C (PLC) pathways notably involve TRPC3 activity. TRPC3 cooperates with the IP3 receptor to mediate calcium release from the endoplasmic reticulum enhancing cellular calcium entry. This interaction ties TRPC3 functionally to the regulation of important physiological processes like cardiac hypertrophy and vascular tone. Additionally TRPC3's relationship with proteins such as calmodulin and STIM1 further illustrates its role in calcium signaling pathways.

Altered TRPC3 activity relates to conditions such as cardiac hypertrophy and neurodegenerative diseases. The protein's dysregulation can result in disrupted calcium homeostasis contributing to excessive cardiac cell growth and potential heart failure. In the nervous system TRPC3 has links to Huntington's disease where its abnormal function associates with neuronal survival and synaptic alterations. The interaction of TRPC3 with proteins such as huntingtin in these pathological contexts highlights its participation in disease development and progression.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Forms a receptor-activated non-selective calcium permeant cation channel. May be operated by a phosphatidylinositol second messenger system activated by receptor tyrosine kinases or G-protein coupled receptors.

See full target information Trpc3

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-TRPC3 antibody [EPR25056-56] (BSA and Azide free)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRPC3 antibody [EPR25056-56] (BSA and Azide free) (AB300143)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRPC3 antibody [EPR25056-56] (BSA and Azide free) (AB300143)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRPC3 antibody [EPR25056-56] (BSA and Azide free) (AB300143)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRPC3 antibody [EPR25056-56] (BSA and Azide free) (AB300143)

Western blot - Anti-TRPC3 antibody [EPR25056-56] (BSA and Azide free) (AB300143)

Western blot - Anti-TRPC3 antibody [EPR25056-56] (BSA and Azide free) (AB300143)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRPC3 antibody [EPR25056-56] (BSA and Azide free) (AB300143)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Complementary Products

Primary Antibodies

Primary Antibodies

Secondary Antibodies

Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Product promise