AB306595

Anti-TRPC5 antibody [N67-15]

BOND RX™ Validated
Recombinant
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(1 Publication)

Mouse Recombinant Monoclonal TRPC5 antibody. Suitable for IHC-P and reacts with Human, Mouse, Rat samples. Cited in 1 publication.

View Alternative Names

TRP5, TRPC5, Short transient receptor potential channel 5, TrpC5, Transient receptor protein 5, TRP-5, hTRP-5, hTRP5

4 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRPC5 antibody [N67-15] (AB306595)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRPC5 antibody [N67-15] (AB306595)

Immunohistochemical analysis of paraffin-embedded human cerebrum tissue labeling TRPC5 with ab306595 at 1/500 dilution (1.714 µg/ml) followed by ready to use LeicaDS9800 (Bond® Polymer Refine Detection). Positive staining on human cerebrum. The section was incubated with ab306595 for 30 mins at room temperature, followed by anti-mouse IgG2b antibody (ab190482) for 8 mins during the LeicaDS9800 kit staining procedure. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is ready to use LeicaDS9800 (Bond® Polymer Refine Detection). Heat mediated antigen retrieval was performed with Citrate buffer (pH 6.0, Epitope Retrieval Solution 1) for 20 mins.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRPC5 antibody [N67-15] (AB306595)

Immunohistochemical analysis of paraffin-embedded (A) HEK-293T cells transfected with a TRPC5 expression vector containing a his tag and (B) HEK-293T cells transfected with empty vector containing a his tag stained for TRPC5 using ab306595 at a 1/5000 dilution. No staining on (B) HEK-293T cells transfected with empty vector containing a his tag. The section was incubated with ab306595 for 30 mins at room temperature, followed by anti-mouse IgG2b antibody (ab190482) for 8 mins during the LeicaDS9800 kit staining procedure. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is ready to use LeicaDS9800 (Bond® Polymer Refine Detection) was used. Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRPC5 antibody [N67-15] (AB306595)

Immunohistochemical analysis of paraffin-embedded rat hippocampus tissue labeling TRPC5 with ab306595 at 1/5000 dilution (1.714 µg/ml) followed by ready to use LeicaDS9800 (Bond® Polymer Refine Detection). Positive staining on rat hippocampus. The section was incubated with ab306595 for 30 mins at room temperature, followed by anti-mouse IgG2b antibody (ab190482) for 8 mins during the LeicaDS9800 kit staining procedure. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is ready to use LeicaDS9800 (Bond® Polymer Refine Detection). Heat mediated antigen retrieval was performed with Citrate buffer (pH 6.0, Epitope Retrieval Solution 1) for 20 mins.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRPC5 antibody [N67-15] (AB306595)

Immunohistochemical analysis of paraffin-embedded mouse hippocampus tissue labeling TRPC5 with ab306595 at 1/5000 dilution (1.714 µg/ml) followed by ready to use LeicaDS9800 (Bond® Polymer Refine Detection). Positive staining on mouse hippocampus. The section was incubated with ab306595 for 30 mins at room temperature, followed by anti-mouse IgG2b antibody (ab190482) for 8 mins during the LeicaDS9800 kit staining procedure. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is ready to use LeicaDS9800 (Bond® Polymer Refine Detection). Heat mediated antigen retrieval was performed with Citrate buffer (pH 6.0, Epitope Retrieval Solution 1) for 20 mins.

Carrier free

Anti-TRPC5 antibody [N67-15] - BSA and Azide Free

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

N67-15

Isotype

IgG2b

Carrier free

Reacts with

Human, Mouse, Rat

Applications

IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/500 - 1/5000", "IHCP-species-notes": "IHC result tested for human cerebrum is positive and shows dilution 1/500 Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "" }, "Mouse": { "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/500 - 1/5000", "IHCP-species-notes": "IHC result tested for human cerebrum is positive and shows dilution 1/500 Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "" }, "Rat": { "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/500 - 1/5000", "IHCP-species-notes": "IHC result tested for human cerebrum is positive and shows dilution 1/500 Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "" } } }

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Product details

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Want a custom formulation?
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

TRPC5 also known as Transient Receptor Potential Canonical 5 functions as a calcium-permeable cation channel. This protein possesses a molecular mass of approximately 105 kDa. TRPC5 is expressed widely with high presence in neural and cardiac tissues. Researchers often investigate its role due to its integral function in calcium ion transport and involvement in neurological activities.

Biological function summary

TRPC5 plays a significant role in the cellular response to stimulating signals. It forms a part of the larger TRPC protein family complex facilitating the entry of calcium ions which are critical for various cellular processes. Activation of TRPC5 influences functions such as synaptic plasticity and neuroprotection. These roles demonstrate the importance of TRPC5 in cellular signaling and homeostasis within the body's systems.

Pathways

TRPC5 integrates into signaling networks like the phosphoinositide pathway. It interacts with proteins such as PKC and DAG which regulate its activity in response to extracellular signals. This interaction bridges TRPC5 to intracellular messaging pathways that mediate cellular growth differentiation and development.

TRPC5 is associated with conditions such as anxiety and cardiovascular diseases. In anxiety abnormal TRPC5 activity alters neuronal transmission processes. Connections between TRPC5 and proteins such as BDNF demonstrate its influence on mood regulation. In cardiovascular diseases altered TRPC5 expression affects blood pressure regulation and vascular function emphasizing its role in maintaining cardiovascular health.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Forms a receptor-activated non-selective calcium permeant cation channel (PubMed : 16284075, PubMed : 38959890, PubMed : 37137991). Mediates calcium-dependent phosphatidylserine externalization and apoptosis in neurons via its association with PLSCR1 (By similarity). Acts on distinct neuronal populations in the hypothalamus to regulate innate behaviors including feeding, anxiety (flight/fight/fear), socialization, and maternal care (By similarity).

See full target information TRPC5

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Translational oncology 39:101809 PubMed37918167

2023

TRPC5 expression promotes the proliferation and invasion of papillary thyroid carcinoma through the HIF-1α/Twist pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Jing Yang,Yue Cheng,Yan Nie,Bole Tian,Jing Huang,Rixiang Gong,Zhihui Li,Jingqiang Zhu,Yanping Gong

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-TRPC5 antibody [N67-15]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRPC5 antibody [N67-15] (AB306595)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRPC5 antibody [N67-15] (AB306595)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRPC5 antibody [N67-15] (AB306595)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRPC5 antibody [N67-15] (AB306595)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Complementary Products

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

Product promise