Mouse Monoclonal TRPC5 antibody. Carrier free. Suitable for IHC-P and reacts with Human, Mouse, Rat samples.
Images
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRPC5 antibody [N67-15] - BSA and Azide Free (AB306596), expandable thumbnail](https://content.abcam.com/products/images/trpc5-antibody-n67-15-bsa-and-azide-free-ab306596--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img404000.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRPC5 antibody [N67-15] - BSA and Azide Free (AB306596)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRPC5 antibody [N67-15] - BSA and Azide Free (AB306596), expandable thumbnail](https://content.abcam.com/products/images/trpc5-antibody-n67-15-bsa-and-azide-free-ab306596--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img403999.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRPC5 antibody [N67-15] - BSA and Azide Free (AB306596)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRPC5 antibody [N67-15] - BSA and Azide Free (AB306596), expandable thumbnail](https://content.abcam.com/products/images/trpc5-antibody-n67-15-bsa-and-azide-free-ab306596--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img403998.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRPC5 antibody [N67-15] - BSA and Azide Free (AB306596)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRPC5 antibody [N67-15] - BSA and Azide Free (AB306596), expandable thumbnail](https://content.abcam.com/products/images/trpc5-antibody-n67-15-bsa-and-azide-free-ab306596--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img403997.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRPC5 antibody [N67-15] - BSA and Azide Free (AB306596)")
Key facts
- Isotype
- IgG2b
- Host species
- Mouse
- Storage buffer
pH: 7.2 - 7.4
Constituents: 100% PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| WB | IHC-P | IP | |
|---|---|---|---|
| Human | Not recommended | Tested | Not recommended |
| Mouse | Not recommended | Tested | Not recommended |
| Rat | Not recommended | Tested | Not recommended |
WB
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat | Dilution info - | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Species Mouse | Dilution info - | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Species Rat | Dilution info - | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
IP
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Human, Rat | Dilution info - | Notes - |
Target data
Function
Forms a receptor-activated non-selective calcium permeant cation channel (PubMed:16284075, PubMed:38959890). Probably is operated by a phosphatidylinositol second messenger system activated by receptor tyrosine kinases or G-protein coupled receptors. Has also been shown to be calcium-selective (By similarity). May also be activated by intracellular calcium store depletion. Mediates calcium-dependent phosphatidylserine externalization and apoptosis in neurons via its association with PLSCR1 (By similarity). Acts on distinct neuronal populations in the hypothalamus to regulate innate behaviors including feeding, anxiety (flight/fight/fear), socialization, and maternal care (By similarity).
Alternative names
TRP5, TRPC5, Short transient receptor potential channel 5, TrpC5, Transient receptor protein 5, TRP-5, hTRP-5, hTRP5
Recommended products
Mouse Monoclonal TRPC5 antibody. Carrier free. Suitable for IHC-P and reacts with Human, Mouse, Rat samples.
Key facts
- Isotype
- IgG2b
- Host species
- Mouse
- Storage buffer
pH: 7.2 - 7.4
Constituents: 100% PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
- Yes
- Clone number
- N67-15
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- Do Not Freeze
Notes
ab306596 ia a carrier free version of Anti-TRPC5 antibody [N67-15] ab306595.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
TRPC5 also known as Transient Receptor Potential Canonical 5 functions as a calcium-permeable cation channel. This protein possesses a molecular mass of approximately 105 kDa. TRPC5 is expressed widely with high presence in neural and cardiac tissues. Researchers often investigate its role due to its integral function in calcium ion transport and involvement in neurological activities.
- Biological function summary
TRPC5 plays a significant role in the cellular response to stimulating signals. It forms a part of the larger TRPC protein family complex facilitating the entry of calcium ions which are critical for various cellular processes. Activation of TRPC5 influences functions such as synaptic plasticity and neuroprotection. These roles demonstrate the importance of TRPC5 in cellular signaling and homeostasis within the body's systems.
- Pathways
TRPC5 integrates into signaling networks like the phosphoinositide pathway. It interacts with proteins such as PKC and DAG which regulate its activity in response to extracellular signals. This interaction bridges TRPC5 to intracellular messaging pathways that mediate cellular growth differentiation and development.
- Associated diseases and disorders
TRPC5 is associated with conditions such as anxiety and cardiovascular diseases. In anxiety abnormal TRPC5 activity alters neuronal transmission processes. Connections between TRPC5 and proteins such as BDNF demonstrate its influence on mood regulation. In cardiovascular diseases altered TRPC5 expression affects blood pressure regulation and vascular function emphasizing its role in maintaining cardiovascular health.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
4 product images
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRPC5 antibody [N67-15] - BSA and Azide Free (ab306596), expandable thumbnail](https://content.abcam.com/products/images/trpc5-antibody-n67-15-bsa-and-azide-free-ab306596--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img404000.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRPC5 antibody [N67-15] - BSA and Azide Free (ab306596)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRPC5 antibody [N67-15] - BSA and Azide Free (ab306596)
This data was developed using Anti-TRPC5 antibody [N67-15] ab306595, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded (A) HEK-293T cells transfected with a TRPC5 expression vector containing a his tag and (B) HEK-293T cells transfected with empty vector containing a his tag stained for TRPC5 using Anti-TRPC5 antibody [N67-15] ab306595 at a 1/5000 dilution.
No staining on (B) HEK-293T cells transfected with empty vector containing a his tag.
The section was incubated with Anti-TRPC5 antibody [N67-15] ab306595 for 30 mins at room temperature, followed by anti-mouse IgG2b antibody (Anti-Mouse IgG2b antibody [RM108] ab190482) for 8 mins during the LeicaDS9800 kit staining procedure. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is ready to use LeicaDS9800 (Bond® Polymer Refine Detection) was used.
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRPC5 antibody [N67-15] - BSA and Azide Free (ab306596), expandable thumbnail](https://content.abcam.com/products/images/trpc5-antibody-n67-15-bsa-and-azide-free-ab306596--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img403999.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRPC5 antibody [N67-15] - BSA and Azide Free (ab306596)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRPC5 antibody [N67-15] - BSA and Azide Free (ab306596)
This data was developed using Anti-TRPC5 antibody [N67-15] ab306595, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded rat hippocampus tissue labeling TRPC5 with Anti-TRPC5 antibody [N67-15] ab306595 at 1/5000 dilution (1.714 µg/ml) followed by ready to use LeicaDS9800 (Bond® Polymer Refine Detection).
Positive staining on rat hippocampus.
The section was incubated with Anti-TRPC5 antibody [N67-15] ab306595 for 30 mins at room temperature, followed by anti-mouse IgG2b antibody (Anti-Mouse IgG2b antibody [RM108] ab190482) for 8 mins during the LeicaDS9800 kit staining procedure. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is ready to use LeicaDS9800 (Bond® Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Citrate buffer (pH 6.0, Epitope Retrieval Solution 1) for 20 mins.
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRPC5 antibody [N67-15] - BSA and Azide Free (ab306596), expandable thumbnail](https://content.abcam.com/products/images/trpc5-antibody-n67-15-bsa-and-azide-free-ab306596--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img403998.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRPC5 antibody [N67-15] - BSA and Azide Free (ab306596)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRPC5 antibody [N67-15] - BSA and Azide Free (ab306596)
This data was developed using Anti-TRPC5 antibody [N67-15] ab306595, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded mouse hippocampus tissue labeling TRPC5 with Anti-TRPC5 antibody [N67-15] ab306595 at 1/5000 dilution (1.714 µg/ml) followed by ready to use LeicaDS9800 (Bond® Polymer Refine Detection).
Positive staining on mouse hippocampus.
The section was incubated with Anti-TRPC5 antibody [N67-15] ab306595 for 30 mins at room temperature, followed by anti-mouse IgG2b antibody (Anti-Mouse IgG2b antibody [RM108] ab190482) for 8 mins during the LeicaDS9800 kit staining procedure. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is ready to use LeicaDS9800 (Bond® Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Citrate buffer (pH 6.0, Epitope Retrieval Solution 1) for 20 mins.
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRPC5 antibody [N67-15] - BSA and Azide Free (ab306596), expandable thumbnail](https://content.abcam.com/products/images/trpc5-antibody-n67-15-bsa-and-azide-free-ab306596--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img403997.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRPC5 antibody [N67-15] - BSA and Azide Free (ab306596)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRPC5 antibody [N67-15] - BSA and Azide Free (ab306596)
This data was developed using Anti-TRPC5 antibody [N67-15] ab306595, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded human cerebrum tissue labeling TRPC5 with Anti-TRPC5 antibody [N67-15] ab306595 at 1/500 dilution (1.714 µg/ml) followed by ready to use LeicaDS9800 (Bond® Polymer Refine Detection).
Positive staining on human cerebrum.
The section was incubated with Anti-TRPC5 antibody [N67-15] ab306595 for 30 mins at room temperature, followed by anti-mouse IgG2b antibody (Anti-Mouse IgG2b antibody [RM108] ab190482) for 8 mins during the LeicaDS9800 kit staining procedure. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is ready to use LeicaDS9800 (Bond® Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Citrate buffer (pH 6.0, Epitope Retrieval Solution 1) for 20 mins.
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