AB228771

Anti-TRPC6 antibody

Name: Anti-TRPC6 antibody (ab228771) | Abcam
Brand: Abcam Limited
SKU: AB228771
Price: 525 USD
Availability: InStock

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(3 Publications)

Rabbit Polyclonal TRPC6 antibody. Suitable for IHC-P, WB and reacts with Mouse, Human samples. Cited in 3 publications. Immunogen corresponding to Recombinant Fragment Protein within Human TRPC6.

View Alternative Names

TRP6, TRPC6, Short transient receptor potential channel 6, TrpC6, Transient receptor protein 6, TRP-6

2 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRPC6 antibody (AB228771)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRPC6 antibody (AB228771)

Paraffin-embedded mouse cerebellum tissue stained TRPC6 at cytoplasm (green) using ab228771 at 1/500. Red : NF-H, a neuron marker, stained by NF-H antibody at 1/500 dilution. Blue : Fluoroshield with DAPI. Antigen Retrieval : Citrate buffer, pH 6.0, 15 min.

Supplier Data

Western blot - Anti-TRPC6 antibody (AB228771)

7.5% SDS-PAGE gel.

All lanes:

Western blot - Anti-TRPC6 antibody (ab228771) at 1/1000 dilution

Lane 1:

A549 (human lung carcinoma cell line) whole cell extract at 30 µg

Lane 2:

A549 membrane extract extract at 30 µg

Predicted band size: 106 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Human

Applications

WB, IHC-P

applications

Immunogen

Recombinant Fragment Protein within Human TRPC6. The exact immunogen used to generate this antibody is proprietary information.

Q9Y210

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>" }, "Mouse": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/1000", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7 Preservative: 0.01% Thimerosal (merthiolate) Constituents: 20% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The TRPC6 also known as Transient Receptor Potential Cation Channel Subfamily C Member 6 functions as a non-selective cation channel permeable to calcium ions. It facilitates calcium entry into cells impacting cellular signaling and homeostasis. TRPC6 has a molecular mass of about 106 kDa. Expression of TRPC6 is broad but is notably significant in tissues such as brain heart lung and skeletal muscle where it influences diverse functions.

Biological function summary

TRPC6 is integral to various cellular processes especially those involving calcium-dependent signaling pathways. It is known to form heteromeric complexes with other TRPC subfamily members such as TRPC3 and TRPC7 which modulates its function. These interactions are important for the regulation of intracellular calcium levels impacting cellular excitability contraction and growth.

Pathways

TRPC6 is involved in the phospholipase C (PLC) pathway and subsequent inositol triphosphate (IP3) signaling. This signaling cascade is vital for the regulation of calcium release from intracellular stores. TRPC6 also has a notable role in the Ras homolog family member A (RhoA) signaling pathway which affects cytoskeletal dynamics and cell motility. Inside these pathways proteins like IP3 receptors and RhoA GTPases are closely related to TRPC6 function.

TRPC6 is associated with conditions like focal segmental glomerulosclerosis (FSGS) and pulmonary hypertension. Mutations in TRPC6 can lead to abnormal regulation of calcium homeostasis in kidney cells contributing to FSGS. Moreover TRPC6 overactivity in pulmonary vascular smooth muscle cells has been implicated in the pathology of pulmonary hypertension. In these conditions TRPC6's interaction with calcineurin and other calcium-dependent signaling proteins plays a critical role in disease progression.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Forms a receptor-activated non-selective calcium permeant cation channel (PubMed : 19936226, PubMed : 23291369, PubMed : 26892346, PubMed : 9930701). Probably is operated by a phosphatidylinositol second messenger system activated by receptor tyrosine kinases or G-protein coupled receptors. Activated by diacylglycerol (DAG) in a membrane-delimited fashion, independently of protein kinase C (PubMed : 26892346). Does not seem to be activated by intracellular calcium store depletion.

See full target information TRPC6

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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Frontiers in neuroscience 17:1302470 PubMed38260021

2024

Characterization of a loss-of-function NSF attachment protein beta mutation in monozygotic triplets affected with epilepsy and autism using cortical neurons from proband-derived and CRISPR-corrected induced pluripotent stem cell lines.

Applications

Unspecified application

Species

Unspecified reactive species

Gowher Ali,Kyung Chul Shin,Wesal Habbab,Ghaneya Alkhadairi,Alice AbdelAleem,Fouad A AlShaban,Yongsoo Park,Lawrence W Stanton

Molecular neurobiology 60:7297-7308 PubMed37552395

2023

Deletion of TRPC6, an Autism Risk Gene, Induces Hyperexcitability in Cortical Neurons Derived from Human Pluripotent Stem Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Kyung Chul Shin,Gowher Ali,Houda Yasmine Ali Moussa,Vijay Gupta,Alberto de la Fuente,Hyung-Goo Kim,Lawrence W Stanton,Yongsoo Park

Frontiers in pharmacology 12:730416 PubMed34880749

2021

Regulation of Cytochrome P450 2a5 by and 6,7-Dimethylesculetin in Mouse Hepatocytes.

Applications

Unspecified application

Species

Unspecified reactive species

Sangsoo Daniel Kim,Larry Morgan,Elyse Hargreaves,Xiaoying Zhang,Zhihui Jiang,Monica Antenos,Ben Li,Gordon M Kirby

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-TRPC6 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRPC6 antibody (AB228771)

Western blot - Anti-TRPC6 antibody (AB228771)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

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Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (3)

Product promise