AB62461

Anti-TRPC6 antibody

Name: Anti-TRPC6 antibody (ab62461) | Abcam
Brand: Abcam Limited
SKU: AB62461
Price: 605 USD
Availability: InStock
Rating: 5 (1 reviews)

(1 Review)

(23 Publications)

Rabbit Polyclonal TRPC6 antibody. Suitable for ICC and reacts with Human samples. Cited in 23 publications. Immunogen corresponding to Synthetic Peptide within Human TRPC6.

View Alternative Names

TRP6, TRPC6, Short transient receptor potential channel 6, TrpC6, Transient receptor protein 6, TRP-6

2 Images

Supplier Data

Immunocytochemistry - Anti-TRPC6 antibody (AB62461)

ICC analysis of K562 (human chronic myelogenous leukemia cell line from bone marrow) cells stained for TRPC6 (red) using ab62461 (5 μg/ml).

Supplier Data

Immunocytochemistry - Anti-TRPC6 antibody (AB62461)

ICC analysis of K562 (human chronic myelogenous leukemia cell line from bone marrow) cells stained for TRPC6 using ab62461 (2 μg/ml).

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

ICC

applications

Immunogen

Synthetic Peptide within Human TRPC6. The exact immunogen used to generate this antibody is proprietary information.

Q9Y210

Specificity

This antibody does potentially cross-react with TRPC3.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICC" : {"fullname" : "Immunocytochemistry", "shortname":"ICC"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICC-species-checked": "testedAndGuaranteed", "ICC-species-dilution-info": "2-5 µg/mL", "ICC-species-notes": "<p></p>", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The TRPC6 also known as Transient Receptor Potential Cation Channel Subfamily C Member 6 functions as a non-selective cation channel permeable to calcium ions. It facilitates calcium entry into cells impacting cellular signaling and homeostasis. TRPC6 has a molecular mass of about 106 kDa. Expression of TRPC6 is broad but is notably significant in tissues such as brain heart lung and skeletal muscle where it influences diverse functions.

Biological function summary

TRPC6 is integral to various cellular processes especially those involving calcium-dependent signaling pathways. It is known to form heteromeric complexes with other TRPC subfamily members such as TRPC3 and TRPC7 which modulates its function. These interactions are important for the regulation of intracellular calcium levels impacting cellular excitability contraction and growth.

Pathways

TRPC6 is involved in the phospholipase C (PLC) pathway and subsequent inositol triphosphate (IP3) signaling. This signaling cascade is vital for the regulation of calcium release from intracellular stores. TRPC6 also has a notable role in the Ras homolog family member A (RhoA) signaling pathway which affects cytoskeletal dynamics and cell motility. Inside these pathways proteins like IP3 receptors and RhoA GTPases are closely related to TRPC6 function.

TRPC6 is associated with conditions like focal segmental glomerulosclerosis (FSGS) and pulmonary hypertension. Mutations in TRPC6 can lead to abnormal regulation of calcium homeostasis in kidney cells contributing to FSGS. Moreover TRPC6 overactivity in pulmonary vascular smooth muscle cells has been implicated in the pathology of pulmonary hypertension. In these conditions TRPC6's interaction with calcineurin and other calcium-dependent signaling proteins plays a critical role in disease progression.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Forms a receptor-activated non-selective calcium permeant cation channel (PubMed : 19936226, PubMed : 23291369, PubMed : 26892346, PubMed : 9930701). Probably is operated by a phosphatidylinositol second messenger system activated by receptor tyrosine kinases or G-protein coupled receptors. Activated by diacylglycerol (DAG) in a membrane-delimited fashion, independently of protein kinase C (PubMed : 26892346). Seems not to be activated by intracellular calcium store depletion.

See full target information TRPC6

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Publications (23)

Recent publications for all applications. Explore the full list and refine your search

ImmunoTargets and therapy 13:735-748 PubMed39691169

2024

TRPC6 is a Biomarker for Prognosis and Immunotherapy of Stomach Adenocarcinoma Based on Bioinformatic Analysis and Experimental Validation.

Applications

Unspecified application

Species

Unspecified reactive species

Xingchi Hu,Hongwei Wang,Haitao Sun,Jingxin Zhang,ZhenXiong Ye,Zhenhua Huang

Kidney360 4:1454-1466 PubMed37265366

2023

Insights into the Regulation of GFR by the Keap1-Nrf2 Pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Kengo Kidokoro,Hiroyuki Kadoya,David Z I Cherney,Megumi Kondo,Yoshihisa Wada,Reina Umeno,Seiji Kishi,Hajime Nagasu,Kojiro Nagai,Takafumi Suzuki,Tamaki Sasaki,Masayuki Yamamoto,Yashpal S Kanwar,Naoki Kashihara

Pharmaceutics 14: PubMed36297636

2022

Polydatin Ameliorates High Fructose-Induced Podocyte Oxidative Stress via Suppressing HIF-1α/NOX4 Pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Hong Ding,Chuanfeng Tang,Wei Wang,Ying Pan,Ruiqing Jiao,Lingdong Kong

Stem cell research & therapy 13:401 PubMed35932080

2022

Role of TRPC6 in periodontal tissue reconstruction mediated by appropriate stress.

Applications

Unspecified application

Species

Unspecified reactive species

Li Wang,Hong Liang,Bingjing Sun,Jing Mi,Xianqin Tong,Yuhui Wang,Meihua Chen,Liming Yu,Jie Pan,Shangfeng Liu,Yan-Jun Liu,Yuehua Liu

Frontiers in cardiovascular medicine 8:731890 PubMed34912856

2021

Hsa_circ_0003204 Knockdown Weakens Ox-LDL-Induced Cell Injury by Regulating miR-188-3p/TRPC6 Axis in Human Carotid Artery Endothelial Cells and THP-1 Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Wenjia Peng,Shuai Li,Shiyue Chen,Jiacheng Yang,Ze Sun

Frontiers in cell and developmental biology 8:594283 PubMed33604333

2021

TRPC6 Attenuates Cortical Astrocytic Apoptosis and Inflammation in Cerebral Ischemic/Reperfusion Injury.

Applications

Unspecified application

Species

Unspecified reactive species

Lu Liu,Manli Chen,Kun Lin,Xuwu Xiang,Jing Yang,Yueying Zheng,Xiaoxing Xiong,Shengmei Zhu

Molecular biology reports 48:1453-1464 PubMed33606151

2021

Astragaloside IV inhibits palmitic acid-induced apoptosis through regulation of calcium homeostasis in mice podocytes.

Applications

Unspecified application

Species

Unspecified reactive species

Yingjun Zang,Shuang Liu,Aili Cao,Xiangyu Shan,Wenjuan Deng,Zhijun Li,Hao Wang,Yunman Wang,Li Wang,Wen Peng

International journal of molecular sciences 22: PubMed33467715

2021

Lithium Reduces Migration and Collagen Synthesis Activity in Human Cardiac Fibroblasts by Inhibiting Store-Operated Ca Entry.

Applications

Unspecified application

Species

Unspecified reactive species

Pao-Huan Chen,Cheng-Chih Chung,Yuan-Feng Lin,Yu-Hsun Kao,Yi-Jen Chen

Shock (Augusta, Ga.) 55:244-255 PubMed33026218

2020

Overexpressed lncRNA AC068039.4 Contributes to Proliferation and Cell Cycle Progression of Pulmonary Artery Smooth Muscle Cells Via Sponging miR-26a-5p/TRPC6 in Hypoxic Pulmonary Arterial Hypertension.

Applications

Unspecified application

Species

Unspecified reactive species

Yuhan Qin,Boqian Zhu,Linqing Li,Dong Wang,Yong Qiao,Bo Liu,Erfei Luo,Jiantong Hou,Gaoliang Yan,Chengchun Tang

Journal of diabetes research 2020:3634974 PubMed33015191

2020

Protective Role of Tangshen Formula on the Progression of Renal Damage in Mice by TRPC6/Talin1 Pathway in Podocytes.

Applications

Unspecified application

Species

Unspecified reactive species

Qian Wang,Xuefei Tian,Wei'e Zhou,Yan Wang,Hailing Zhao,Jialin Li,Xuefeng Zhou,Haojun Zhang,Tingting Zhao,Ping Li

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-TRPC6 antibody

Immunocytochemistry - Anti-TRPC6 antibody (AB62461)

Immunocytochemistry - Anti-TRPC6 antibody (AB62461)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Specificity

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (23)

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