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AB219965

Anti-TRPM1 antibody

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Rabbit Polyclonal TRPM1 antibody. Suitable for IHC-P and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human TRPM1 aa 1250-1400.

View Alternative Names

LTRPC1, MLSN, MLSN1, TRPM1, Transient receptor potential cation channel subfamily M member 1, Long transient receptor potential channel 1, Melastatin-1, LTrpC1

1 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRPM1 antibody (AB219965)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRPM1 antibody (AB219965)

Immunohistochemical analysis of human retina tissue labeling TRPM1 in the cytoplasm of inner nuclear layer and outer plexiform layer with ab219965 at a 1/500 dilution.

Performed heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

IHC-P

applications

Immunogen

Recombinant Fragment Protein within Human TRPM1 aa 1250-1400. The exact immunogen used to generate this antibody is proprietary information.

Q7Z4N2

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/500 - 1/1000", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol." } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

TRPM1 also known as transient receptor potential melastatin 1 or melanoma-associated antigen recognized by T-cells 1 (MART-1) is a calcium-permeable ion channel. It plays a role in calcium and other divalent cations transportation across cell membranes. TRPM1 is a large protein with a molecular mass of approximately 180 kDa. TRPM1 expresses mainly in the retina specifically in bipolar cells and also shows expression in melanocytes. This channel participates in synaptic signaling in the visual system.
Biological function summary

TRPM1 is involved in normal retinal function and visual signal transduction. It partners with other synaptic proteins to form complexes that contribute to synaptic transmission in the retina. In retinal bipolar cells TRPM1 mediates the synaptic response to light by modulating intracellular calcium levels and is important for visual signal processing. In melanocytes TRPM1 may play a role in melanin production linking it to pigmentation processes.

Pathways

TRPM1 plays an important role in the mGluR6 signaling pathway in the retina where it directly affects synaptic transmission in rod bipolar cells. The pathway involves metabotropic glutamate receptor 6 (mGluR6) that changes the cell’s response to light exposure. Additionally TRPM1 interacts with RGS proteins including RGS7 and RGS11 which regulate this signaling pathway by modulating the function of the associated G-proteins. This modulation is critical for precise tuning of the visual signal transduction.

Mutations in TRPM1 have been linked to congenital stationary night blindness (CSNB) a condition marked by impaired vision under low light conditions. TRPM1 mutations disrupt normal channel function preventing appropriate signal transmission in the retinal cells. Additionally aberrant expression of TRPM1 correlates with melanoma. The low expression levels of TRPM1 in melanoma cells suggest a potential role as a prognostic marker in melanoma progression. In these contexts TRPM1 relationships with G-protein signaling and interaction with mGluR6 and RGS proteins may also contribute to the disease pathology.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Constitutively open nonselective divalent cation-conducting channels which mediate the influx of Ca(2+), Mg(2+), Mn(2+), Ba(2+), and Ni(2+) into the cytoplasm, leading to membrane depolarization (PubMed : 11535825, PubMed : 19436059, PubMed : 21278253). Impermeable to zinc ions (PubMed : 21278253). In addition, forms heteromultimeric ion channels with TRPM3 which are permeable for calcium and zinc ions (PubMed : 21278253). Plays an essential role for the depolarizing photoresponse of retinal ON bipolar cells (PubMed : 19878917, PubMed : 19896109). In the dark, tonic release of glutamate activates the G-protein coupled receptor for glutamate, GRM6, its activation induces the release of G(o) protein and the beta-gamma G protein dimer. Both subunits can interact and inactivate the TRPM1 channel. A light onset, induces decrease in glutamate release and deactivation of GRM6 leading to channel opening and membrane depolarization (By similarity). May play a role in metastasis suppression (PubMed : 9537257).
See full target information TRPM1
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