AB11168

Anti-TRPM2 antibody

BOND RX™ Validated
What is this?

(2 Reviews)

(27 Publications)

Rabbit Polyclonal TRPM2 antibody. Suitable for IP, WB, IHC-P and reacts with Human, Rat samples. Cited in 27 publications. Immunogen corresponding to Synthetic Peptide within Human TRPM2 aa 650-700.

View Alternative Names

EREG1, KNP3, LTRPC2, TRPC7, TRPM2, Transient receptor potential cation channel subfamily M member 2, Estrogen-responsive element-associated gene 1 protein, Long transient receptor potential channel 2, Transient receptor potential channel 7, Transient receptor potential melastatin 2, LTrpC-2, LTrpC2, TrpC7

3 Images

Supplier Data

Immunoprecipitation - Anti-TRPM2 antibody (AB11168)

Detection of human TRPM2 by western blot of immunoprecipitates. Samples : Membrane Prep (0.5 or 1.0mg per IP reaction; 20% of IP loaded) from Jurkat cells. Antibodies : Affinity purified rabbit anti-TRPM2 antibody A300-414A (lot A300-414A-3) used for IP at 6ug per reaction. TRPM2 was also immunoprecipitated by rabbit anti-TRPM2 antibody A300-413A. For blotting immunoprecipitated TRPM2, A300-414A was used at 1 ug/ml. Detection : Chemiluminescence with an exposure time of 30 seconds.

All lanes:

Immunoprecipitation - Anti-TRPM2 antibody (ab11168)

Predicted band size: 171 kDa

false

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRPM2 antibody (AB11168)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRPM2 antibody (AB11168)

IHC image of ab11168 staining in rat brain formalin fixed paraffin embedded tissue section, performed on a Leica Bond^TM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab11168, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

Supplier Data

Western blot - Anti-TRPM2 antibody (AB11168)

All lanes:

Western blot - Anti-TRPM2 antibody (ab11168) at 0.1 µg

Lane 1:

Jurkat (human T cell leukemia cell line from peripheral blood) membrane preparation at 50 µg

Lane 2:

Jurkat membrane preparation at 15 µg

Lane 3:

Jurkat membrane preparation at 5 µg

Predicted band size: 171 kDa

true

Exposure time: 3min

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Rat, Human

Applications

IHC-P, WB, IP

applications

Immunogen

Synthetic Peptide within Human TRPM2 aa 650-700. The exact immunogen used to generate this antibody is proprietary information.

O94759

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1-4 µg", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/300", "WB-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Mouse": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1 µg/mL", "IHCP-species-notes": "" }, "Chimpanzee": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Elephant": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Gorilla": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Orangutan": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rhesus monkey": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Purification notes

Antibodies were affinity purified using the peptide immobilized on solid support.

Storage buffer

pH: 7 - 8 Preservative: 0.1% Sodium azide Constituents: PBS, 1.815% Tris, 1.764% Sodium citrate

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle|Do Not Freeze

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

TRPM2 also known as Transient Receptor Potential Melastatin 2 is a calcium-permeable non-selective cation channel. Its molecular weight is approximately 171 kDa. TRPM2 is mainly expressed in the brain immune cells and pancreas. It responds to oxidative stress and other physiological stimuli facilitating calcium influx into the cell. This channel is involved in cellular signaling by mediating calcium entry triggered by hydrogen peroxide and ADP-ribose.

Biological function summary

This channel influences various cellular processes including apoptosis cytokine production and insulin secretion. As a part of a larger protein complex TRPM2 interacts with calmodulin and other related proteins to regulate calcium signaling. Its function in immune cells involves modulation of inflammation while in the pancreas it helps in regulating insulin release in response to glucose levels.

Pathways

TRPM2 plays a significant role in the reactive oxygen species (ROS) signaling pathway and the NF-κB pathway. It shares a connection with proteins like p38 MAPK and caspase proteins which are critical in mediating stress responses and apoptotic pathways. These interactions allow TRPM2 to act as a sensor and effector of cellular oxidative conditions triggering downstream signaling cascades.

Scientists associate TRPM2 with neurodegenerative disorders like Alzheimer's disease and conditions involving insulin resistance such as Type 2 diabetes. The channel's link to the Alzheimer's condition involves its interaction with amyloid-beta while in diabetes it relates to proteins such as insulin receptor substrate (IRS) proteins. Understanding these associations helps in exploring TRPM2 as a potential therapeutic target.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Isoform 1. Nonselective, voltage-independent cation channel that mediates Na(+) and Ca(2+) influx, leading to increased cytoplasmic Ca(2+) levels (PubMed : 11385575, PubMed : 11509734, PubMed : 11804595, PubMed : 12594222, PubMed : 15561722, PubMed : 16601673, PubMed : 19171771, PubMed : 20660597, PubMed : 25620041, PubMed : 27068538, PubMed : 27383051, PubMed : 28775320, PubMed : 29745897, PubMed : 30467180, PubMed : 31513012, PubMed : 34788616). Functions as a ligand-gated ion channel, gated by intracellular adenosine diphosphate ribose (ADP-ribose), Ca(2+), warm temperature, and oxidative stress (PubMed : 19171771, PubMed : 25620041, PubMed : 28775320, PubMed : 30467180). The precise physiological activators are under debate; the true, physiological activators may be ADP-ribose and ADP-ribose-2'-phosphate (PubMed : 20650899, PubMed : 25918360). Binding of ADP-ribose to the cytoplasmic Nudix domain causes a conformation change; the channel is primed but still requires Ca(2+) binding to trigger channel opening (PubMed : 19171771, PubMed : 25620041, PubMed : 28775320, PubMed : 29745897, PubMed : 30467180). Extracellular Ca(2+) passes through the channel and increases channel activity (PubMed : 19171771). Contributes to Ca(2+) release from intracellular stores in response to ADP-ribose (PubMed : 19454650). Plays a role in numerous processes that involve signaling via intracellular Ca(2+) levels (Probable). Besides, mediates the release of lysosomal Zn(2+) stores in response to reactive oxygen species, leading to increased cytosolic Zn(2+) levels (PubMed : 25562606, PubMed : 27068538). Plays a role in mediating behavorial and physiological responses to moderate heat and thereby contributes to body temperature homeostasis. Plays a role in insulin secretion, a process that requires increased cytoplasmic Ca(2+) levels (By similarity). Required for normal IFNG and cytokine secretion and normal innate immune immunity in response to bacterial infection. Required for normal phagocytosis and cytokine release by macrophages exposed to zymosan (in vitro) (PubMed : 22493272). Plays a role in dendritic cell differentiation and maturation, and in dendritic cell chemotaxis via its role in regulating cytoplasmic Ca(2+) levels (By similarity). Plays a role in the regulation of the reorganization of the actin cytoskeleton and filopodia formation in response to reactive oxygen species via its role in increasing cytoplasmic Ca(2+) and Zn(2+) levels (PubMed : 27068538). Confers susceptibility to cell death following oxidative stress (PubMed : 12594222, PubMed : 25562606).. Isoform 2. Lacks cation channel activity. Does not mediate cation transport in response to oxidative stress or ADP-ribose.. Isoform 3. Lacks cation channel activity and negatively regulates the channel activity of isoform 1. Negatively regulates susceptibility to cell death in reposponse to oxidative stress.

See full target information TRPM2

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Publications (27)

Recent publications for all applications. Explore the full list and refine your search

European journal of trauma and emergency surgery : official publication of the European Trauma Society 51:241 PubMed40576729

2025

Protective effects of tasimelteon on kidney injury in a traumatic brain injury rat model: a histopathological and immunohistochemical study.

Applications

Unspecified application

Species

Unspecified reactive species

Adem Milletsever,Halil Asci,Rumeysa Taner,Ozlem Ozmen

European journal of histochemistry : EJH 68: PubMed39526436

2024

Activation of Hedgehog pathway by circEEF2/miR-625-5p/TRPM2 axis promotes prostate cancer cell proliferation through mitochondrial stress.

Applications

Unspecified application

Species

Unspecified reactive species

ChenHui Zhu,LiJuan Lin,ChangQing Huang,ZhiHui Wu

Environmental toxicology 40:222-244 PubMed39387650

2024

Evaluation of TRPM2 Channel-Mediated Autophagic Signaling Pathway in Hippocampus and Cortex Tissues of Rat Offspring Following Prenatal Exposure to Elevated Alcohol Levels.

Applications

Unspecified application

Species

Unspecified reactive species

Abdülhadi Cihangir Uğuz,Aslı Okan,Züleyha Doğanyiğit,Seher Yilmaz,Şükrü Ateş,Evrim Suna Arikan Söylemez,Sebahattin Karabulut,Alper Serhat Kumru,Javier Espino

Clinical and experimental pharmacology & physiology 51:e13844 PubMed38350599

2024

Synergic actions of botulinum neurotoxin A and oxaliplatin on colorectal tumour cell death through the upregulation of TRPM2 channel-mediated oxidative stress.

Applications

Unspecified application

Species

Unspecified reactive species

Sıdıka Demir,İpek Duman,Mustafa Nazıroğlu

Antioxidants (Basel, Switzerland) 11: PubMed36421426

2022

Citronellal Attenuates Oxidative Stress-Induced Mitochondrial Damage through TRPM2/NHE1 Pathway and Effectively Inhibits Endothelial Dysfunction in Type 2 Diabetes Mellitus.

Applications

Unspecified application

Species

Unspecified reactive species

Ya-Ling Yin,Huan-Huan Wang,Zi-Chen Gui,Shan Mi,Shuang Guo,Yue Wang,Qian-Qian Wang,Rui-Zhu Yue,Lai-Biao Lin,Jia-Xin Fan,Xue Zhang,Bing-Yan Mao,Tian-Heng Liu,Guang-Rui Wan,He-Qin Zhan,Mo-Li Zhu,Lin-Hua Jiang,Peng Li

Cell communication and signaling : CCS 20:128 PubMed36008839

2022

The Janus-faced role of TRPM2-S in retroperitoneal liposarcoma via increasing ROS levels.

Applications

Unspecified application

Species

Unspecified reactive species

Xiangji Li,Fanqin Bu,Shixiang Ma,Ferdinando Cananzi,Yu Zhao,Mengmeng Xiao,Li Min,Chenghua Luo

Pharmaceutical biology 60:1365-1373 PubMed35881053

2022

Improvement of irradiation-induced fibroblast damage by α2-macroglobulin through alleviating mitochondrial dysfunction.

Applications

Unspecified application

Species

Unspecified reactive species

Chaoji Huangfu,Nan Tang,Xiaokun Yang,Zhanwei Gong,Junzheng Li,Junting Jia,Jingang Zhang,Yan Huang,Yuyuan Ma

Journal of viral hepatitis 29:627-636 PubMed35633088

2022

TRPM2 regulates autophagy to participate in hepatitis B virus replication.

Applications

Unspecified application

Species

Unspecified reactive species

Liyan Chen,Liying Zhu,Xingyu Lu,Xiaoyu Ming,Baoshan Yang

Molecular biology reports 49:7337-7345 PubMed35585377

2022

Umbilical cord-derived mesenchymal stem cell conditioned medium reverses neuronal oxidative injury by inhibition of TRPM2 activation and the JNK signaling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Yan Wang,Jiaxin Liu,Baocong Yu,Yiran Jin,Jiahui Li,Xiaona Ma,Jianqiang Yu,Jianguo Niu,Xueyun Liang

International journal of developmental neuroscience : the official journal of the International Society for Developmental Neuroscience 82:231-242 PubMed35261075

2022

Maternal prenatal stress and depression-like behavior associated with hippocampal and cortical neuroinflammation in the offspring: An experimental study.

Applications

Unspecified application

Species

Unspecified reactive species

Gülin Özdamar Ünal,Kuyaş Hekimler Öztürk,Gamze Erkılınç,Feyza Dönmez,Duygu Kumbul Doğuç,Özlem Özmen,Mekin Sezik

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Anti-TRPM2 antibody

Immunoprecipitation - Anti-TRPM2 antibody (AB11168)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRPM2 antibody (AB11168)

Western blot - Anti-TRPM2 antibody (AB11168)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Alternative Products

Primary Antibodies

Complementary Products

Primary Antibodies

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (27)

Product promise