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AB326596

Anti-TRPM2 antibody [EPR22476-57] - BSA and Azide free

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Rabbit Recombinant Monoclonal TRPM2 antibody. Carrier free. Suitable for WB and reacts with Human samples.

View Alternative Names

EREG1, KNP3, LTRPC2, TRPC7, TRPM2, Transient receptor potential cation channel subfamily M member 2, Estrogen-responsive element-associated gene 1 protein, Long transient receptor potential channel 2, Transient receptor potential channel 7, Transient receptor potential melastatin 2, LTrpC-2, LTrpC2, TrpC7

3 Images
Western blot - Anti-TRPM2 antibody [EPR22476-57] - BSA and Azide free (AB326596)
  • WB

Supplier Data

Western blot - Anti-TRPM2 antibody [EPR22476-57] - BSA and Azide free (AB326596)

This data was developed using ab324384, the same antibody clone in a different buffer formulation.

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

Low expression : Raji RKO.

Samples are non-boiled as boiling may cause protein aggregation.

In Western blot Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.

All lanes:

Western blot - Anti-TRPM2 antibody [EPR22476-57] (<a href='/en-us/products/primary-antibodies/trpm2-antibody-epr22476-57-ab324384'>ab324384</a>) at 1/1000 dilution

Lane 1:

THP-1 (human monocytic leukemia monocyte) whole cell lysate at 80 µg

Lane 2:

Raji (human Burkitt's lymphoma B lymphocyte) whole cell lysate at 80 µg

Lane 3:

RKO (human colon epithelial) whole cell lysate at 80 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 171 kDa,36 kDa

false

Exposure time: 10s

Western blot - Anti-TRPM2 antibody [EPR22476-57] - BSA and Azide free (AB326596)
  • WB

Supplier Data

Western blot - Anti-TRPM2 antibody [EPR22476-57] - BSA and Azide free (AB326596)

This data was developed using ab324384, the same antibody clone in a different buffer formulation.

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

Low expression : liver lung (PMID : 14512294 PMID : 9806837)

Samples are non-boiled as boiling may cause protein aggregation.

The identity of the bands between 15 kDa and 75 kDa are unknown.

In Western blot Anti-Vinculin antibody [EPR8185] (ab129002) staining at 1/10000 dilution.

All lanes:

Western blot - Anti-TRPM2 antibody [EPR22476-57] (<a href='/en-us/products/primary-antibodies/trpm2-antibody-epr22476-57-ab324384'>ab324384</a>) at 1/1000 dilution

Lane 1:

Human cerebellum tissue lysate at 80 µg

Lane 2:

Human lung tissue lysate at 80 µg

Lane 3:

Human liver lysate at 80 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

Observed band size: 171 kDa,124 kDa

false

Exposure time: 37s

Western blot - Anti-TRPM2 antibody [EPR22476-57] - BSA and Azide free (AB326596)
  • WB

Supplier Data

Western blot - Anti-TRPM2 antibody [EPR22476-57] - BSA and Azide free (AB326596)

This data was developed using ab324384, the same antibody clone in a different buffer formulation.

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

Samples are non-boiled as boiling may cause protein aggregation.

In Western blot Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.

All lanes:

Western blot - Anti-TRPM2 antibody [EPR22476-57] (<a href='/en-us/products/primary-antibodies/trpm2-antibody-epr22476-57-ab324384'>ab324384</a>) at 1/1000 dilution

Lane 1:

THP-1 (human monocytic leukemia monocyte) transfected with scrambled siRNA control whole cell lysate at 80 µg

Lane 2:

THP-1 transfected with siRNA specifically targeting TRPM2 whole cell lysate at 80 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 171 kDa,36 kDa

false

Exposure time: 26s

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR22476-57

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCFr" : {"fullname" : "Immunohistochemistry (Frozen sections)", "shortname":"IHC-Fr"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IHCFr-species-checked": "notRecommended", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IHCFr-species-checked": "notRecommended", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" } } }
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Product details

ab326596 is the carrier-free version of ab324384

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Isoform 1. Nonselective, voltage-independent cation channel that mediates Na(+) and Ca(2+) influx, leading to increased cytoplasmic Ca(2+) levels (PubMed : 11385575, PubMed : 11509734, PubMed : 11804595, PubMed : 12594222, PubMed : 15561722, PubMed : 16601673, PubMed : 19171771, PubMed : 20660597, PubMed : 25620041, PubMed : 27068538, PubMed : 27383051, PubMed : 28775320, PubMed : 29745897, PubMed : 30467180, PubMed : 31513012, PubMed : 34788616). Functions as a ligand-gated ion channel, gated by intracellular adenosine diphosphate ribose (ADP-ribose), Ca(2+), warm temperature, and oxidative stress (PubMed : 19171771, PubMed : 25620041, PubMed : 28775320, PubMed : 30467180). The precise physiological activators are under debate; the true, physiological activators may be ADP-ribose and ADP-ribose-2'-phosphate (PubMed : 20650899, PubMed : 25918360). Binding of ADP-ribose to the cytoplasmic Nudix domain causes a conformation change; the channel is primed but still requires Ca(2+) binding to trigger channel opening (PubMed : 19171771, PubMed : 25620041, PubMed : 28775320, PubMed : 29745897, PubMed : 30467180). Extracellular Ca(2+) passes through the channel and increases channel activity (PubMed : 19171771). Contributes to Ca(2+) release from intracellular stores in response to ADP-ribose (PubMed : 19454650). Plays a role in numerous processes that involve signaling via intracellular Ca(2+) levels (Probable). Besides, mediates the release of lysosomal Zn(2+) stores in response to reactive oxygen species, leading to increased cytosolic Zn(2+) levels (PubMed : 25562606, PubMed : 27068538). Plays a role in mediating behavorial and physiological responses to moderate heat and thereby contributes to body temperature homeostasis. Plays a role in insulin secretion, a process that requires increased cytoplasmic Ca(2+) levels (By similarity). Required for normal IFNG and cytokine secretion and normal innate immune immunity in response to bacterial infection. Required for normal phagocytosis and cytokine release by macrophages exposed to zymosan (in vitro) (PubMed : 22493272). Plays a role in dendritic cell differentiation and maturation, and in dendritic cell chemotaxis via its role in regulating cytoplasmic Ca(2+) levels (By similarity). Plays a role in the regulation of the reorganization of the actin cytoskeleton and filopodia formation in response to reactive oxygen species via its role in increasing cytoplasmic Ca(2+) and Zn(2+) levels (PubMed : 27068538). Confers susceptibility to cell death following oxidative stress (PubMed : 12594222, PubMed : 25562606).. Isoform 2. Lacks cation channel activity. Does not mediate cation transport in response to oxidative stress or ADP-ribose.. Isoform 3. Lacks cation channel activity and negatively regulates the channel activity of isoform 1. Negatively regulates susceptibility to cell death in reposponse to oxidative stress.
See full target information TRPM2
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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