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AB247024

Anti-TRPM4 antibody

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Rabbit Polyclonal TRPM4 antibody. Suitable for IHC-P, ICC/IF and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human TRPM4 aa 250-400.

View Alternative Names

LTRPC4, TRPM4, Transient receptor potential cation channel subfamily M member 4, hTRPM4, Calcium-activated non-selective cation channel 1, Long transient receptor potential channel 4, Melastatin-4, LTrpC-4, LTrpC4

4 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRPM4 antibody (AB247024)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRPM4 antibody (AB247024)

Paraffin embedded human prostate tissue stained for TRPM4 using ab247024 at 1/1000 dilution in immunohistochemical analysis.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRPM4 antibody (AB247024)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRPM4 antibody (AB247024)

Paraffin embedded human liver tissue stained for TRPM4 using ab247024 at 1/1000 dilution in immunohistochemical analysis. It shows no positivity in hepatocytes as expected.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRPM4 antibody (AB247024)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRPM4 antibody (AB247024)

Paraffin embedded human colon tissue stained for TRPM4 using ab247024 at 1/1000 dilution in immunohistochemical analysis.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRPM4 antibody (AB247024)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRPM4 antibody (AB247024)

Paraffin embedded human kidney tissue stained for TRPM4 using ab247024 at 1/1000 dilution in immunohistochemical analysis.

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

ICC/IF, IHC-P

applications

Immunogen

Recombinant Fragment Protein within Human TRPM4 aa 250-400. The exact immunogen used to generate this antibody is proprietary information.

Q8TD43

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/500 - 1/1000", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "0.25-2 µg/mL", "ICCIF-species-notes": "<p>Fixation/Permeabilization: PFA/Triton X-100.</p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

TRPM4 also known as Transient Receptor Potential Melastatin 4 is a calcium-activated non-selective cation channel with a mass of approximately 138 kilodaltons. This protein allows the movement of monovalent cations such as sodium across the plasma membrane leading to membrane depolarization. TRPM4 expresses in numerous human tissues including the heart brain and immune cells. Researchers often study this channel to understand its role in cellular electric activities.
Biological function summary

TRPM4 plays an important part in regulating membrane potential and cellular excitability. It does not function as part of a larger structural complex but its activity modulates various cellular processes. For instance it is important in cardiac conduction influencing heart rhythm by affecting the action potential duration. In immune cells TRPM4 contributes to cytokine release and cell movement impacting the body’s inflammatory response.

Pathways

TRPM4's mechanism integrates into significant signaling pathways like the calcium signaling pathway and the cardiac conduction system. Within these pathways its function influences several key proteins. In the calcium signaling pathway TRPM4's activity can affect the function of calmodulin an important calcium sensor while in cardiac cells TRPM4 regulates voltage-gated calcium channels important for heart muscle contraction.

TRPM4’s roles associate it with conditions like cardiac arrhythmias and multiple sclerosis. In heart diseases TRPM4 interacts with proteins such as Na+/Ca2+ exchangers and abnormalities in this interaction can lead to arrhythmogenic conditions. For multiple sclerosis studies suggest that altered TRPM4 activity in immune cells impacts the immune response potentially linking it to the disease's progression. Understanding these interactions can provide insights for therapeutic targets.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Calcium-activated selective cation channel that mediates membrane depolarization (PubMed : 12015988, PubMed : 12842017, PubMed : 29211723, PubMed : 30528822). While it is activated by increase in intracellular Ca(2+), it is impermeable to it (PubMed : 12015988). Mediates transport of monovalent cations (Na(+) > K(+) > Cs(+) > Li(+)), leading to depolarize the membrane (PubMed : 12015988). It thereby plays a central role in cadiomyocytes, neurons from entorhinal cortex, dorsal root and vomeronasal neurons, endocrine pancreas cells, kidney epithelial cells, cochlea hair cells etc. Participates in T-cell activation by modulating Ca(2+) oscillations after T lymphocyte activation, which is required for NFAT-dependent IL2 production. Involved in myogenic constriction of cerebral arteries. Controls insulin secretion in pancreatic beta-cells. May also be involved in pacemaking or could cause irregular electrical activity under conditions of Ca(2+) overload. Affects T-helper 1 (Th1) and T-helper 2 (Th2) cell motility and cytokine production through differential regulation of calcium signaling and NFATC1 localization. Enhances cell proliferation through up-regulation of the beta-catenin signaling pathway. Plays a role in keratinocyte differentiation (PubMed : 30528822).. Isoform 2. Lacks channel activity.
See full target information TRPM4
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