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AB327132

Anti-TRPM4 Antibody [CL11214]

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Mouse Monoclonal TRPM4 antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human TRPM4.

View Alternative Names

LTRPC4, TRPM4, Transient receptor potential cation channel subfamily M member 4, hTRPM4, Calcium-activated non-selective cation channel 1, Long transient receptor potential channel 4, Melastatin-4, LTrpC-4, LTrpC4

7 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRPM4 Antibody [CL11214] (AB327132)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRPM4 Antibody [CL11214] (AB327132)

Immunohistochemistry analysis in human duodenum and pancreas tissues using ab327132 at 1 : 50–1 : 200 dilution. Corresponding TRPM4 RNA-seq data are presented for the same tissues.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRPM4 Antibody [CL11214] (AB327132)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRPM4 Antibody [CL11214] (AB327132)

Immunohistochemical staining of human prostate using ab327132 at 1 : 50–1 : 200 dilution, showing moderate membranous positivity in glandular cells.

Immunocytochemistry/ Immunofluorescence - Anti-TRPM4 Antibody [CL11214] (AB327132)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-TRPM4 Antibody [CL11214] (AB327132)

Immunofluorescence staining of MCF7 cells using ab327132 at 2–10 µg/ml, showing specific staining in the plasma membrane in green. Microtubule- and nuclear probes are visualized in red and blue, respectively (where available).

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRPM4 Antibody [CL11214] (AB327132)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRPM4 Antibody [CL11214] (AB327132)

Immunohistochemical staining of human duodenum using ab327132 at 1 : 50–1 : 200 dilution, showing strong membranous positivity in glandular cells.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRPM4 Antibody [CL11214] (AB327132)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRPM4 Antibody [CL11214] (AB327132)

Immunohistochemical staining of human kidney using ab327132 at 1 : 50–1 : 200 dilution, showing moderate to strong membranous positivity in cells in tubules.

Western blot - Anti-TRPM4 Antibody [CL11214] (AB327132)
  • WB

Supplier Data

Western blot - Anti-TRPM4 Antibody [CL11214] (AB327132)

Western blot analysis in human cell line RT-4 using ab327132 at 1 µg/ml.

All lanes:

Western blot - Anti-TRPM4 Antibody [CL11214] (ab327132)

All lanes:

Human cell line RT-4

Predicted band size: 134.3 kDa

false

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRPM4 Antibody [CL11214] (AB327132)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRPM4 Antibody [CL11214] (AB327132)

Immunohistochemical staining of human pancreas using ab327132 at 1 : 50–1 : 200 dilution, showing no positivity in exocrine glandular cells as expected.

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

CL11214

Isotype

IgG1

Carrier free

No

Reacts with

Human

Applications

WB, ICC/IF, IHC-P

applications

Immunogen

Recombinant Fragment Protein within Human TRPM4.

Q8TD43

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1 µg/mL", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50 - 1/200", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval before commencing with IHC staining protocol.", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "2-10 µg/mL", "ICCIF-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)
Shipped at conditions
Ambient - Can Ship with Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Calcium-activated selective cation channel that mediates membrane depolarization (PubMed : 12015988, PubMed : 12842017, PubMed : 29211723, PubMed : 30528822). While it is activated by increase in intracellular Ca(2+), it is impermeable to it (PubMed : 12015988). Mediates transport of monovalent cations (Na(+) > K(+) > Cs(+) > Li(+)), leading to depolarize the membrane (PubMed : 12015988). It thereby plays a central role in cadiomyocytes, neurons from entorhinal cortex, dorsal root and vomeronasal neurons, endocrine pancreas cells, kidney epithelial cells, cochlea hair cells etc. Participates in T-cell activation by modulating Ca(2+) oscillations after T lymphocyte activation, which is required for NFAT-dependent IL2 production. Involved in myogenic constriction of cerebral arteries. Controls insulin secretion in pancreatic beta-cells. May also be involved in pacemaking or could cause irregular electrical activity under conditions of Ca(2+) overload. Affects T-helper 1 (Th1) and T-helper 2 (Th2) cell motility and cytokine production through differential regulation of calcium signaling and NFATC1 localization. Enhances cell proliferation through up-regulation of the beta-catenin signaling pathway. Plays a role in keratinocyte differentiation (PubMed : 30528822).. Isoform 2. Lacks channel activity.
See full target information TRPM4
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Product promise

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