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Mouse Monoclonal TRPM4 antibody. Suitable for IHC-P, WB and reacts with Human, Dog, African green monkey, Transfected cell lysate - Human samples. Cited in 3 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human TRPM4.

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Images

Western blot - Anti-TRPM4 antibody [OTI10H5] (AB123936), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRPM4 antibody [OTI10H5] (AB123936), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRPM4 antibody [OTI10H5] (AB123936), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRPM4 antibody [OTI10H5] (AB123936), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRPM4 antibody [OTI10H5] (AB123936), expandable thumbnail

Publications

Key facts

Isotype
IgG1
Host species
Mouse
Storage buffer

pH: 7.3
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine), 1% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • Recombinant Full Length Protein corresponding to Human TRPM4. Database link Q8TD43

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IHC-PWB
Human
Tested
Tested
African green monkey
Expected
Tested
Dog
Expected
Tested
Transfected cell lysate - Human
Not recommended
Tested

Tested
Tested

Species
Human
Dilution info
1/50
Notes

Perform heat-mediated antigen retrieval before commencing with IHC staining protocol.

Expected
Expected

Species
Dog, African green monkey
Dilution info
Use at an assay dependent concentration.
Notes

-

Not recommended
Not recommended

Species
Transfected cell lysate - Human
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
1/5000.00000 - 1/10000.00000
Notes

-

Species
Dog
Dilution info
1/5000.00000 - 1/10000.00000
Notes

-

Species
African green monkey
Dilution info
1/5000.00000 - 1/10000.00000
Notes

-

Species
Transfected cell lysate - Human
Dilution info
1/5000.00000 - 1/10000.00000
Notes

-

Associated Products

Select an associated product type

1 product for Alternative Product

Target data

Function

Calcium-activated selective cation channel that mediates membrane depolarization (PubMed:12015988, PubMed:12842017, PubMed:29211723, PubMed:30528822). While it is activated by increase in intracellular Ca(2+), it is impermeable to it (PubMed:12015988). Mediates transport of monovalent cations (Na(+) > K(+) > Cs(+) > Li(+)), leading to depolarize the membrane (PubMed:12015988). It thereby plays a central role in cadiomyocytes, neurons from entorhinal cortex, dorsal root and vomeronasal neurons, endocrine pancreas cells, kidney epithelial cells, cochlea hair cells etc. Participates in T-cell activation by modulating Ca(2+) oscillations after T lymphocyte activation, which is required for NFAT-dependent IL2 production. Involved in myogenic constriction of cerebral arteries. Controls insulin secretion in pancreatic beta-cells. May also be involved in pacemaking or could cause irregular electrical activity under conditions of Ca(2+) overload. Affects T-helper 1 (Th1) and T-helper 2 (Th2) cell motility and cytokine production through differential regulation of calcium signaling and NFATC1 localization. Enhances cell proliferation through up-regulation of the beta-catenin signaling pathway. Plays a role in keratinocyte differentiation (PubMed:30528822). Isoform 2. Lacks channel activity.

Alternative names

LTRPC4, TRPM4, Transient receptor potential cation channel subfamily M member 4, hTRPM4, Calcium-activated non-selective cation channel 1, Long transient receptor potential channel 4, Melastatin-4, LTrpC-4, LTrpC4

Recommended products

Mouse Monoclonal TRPM4 antibody. Suitable for IHC-P, WB and reacts with Human, Dog, African green monkey, Transfected cell lysate - Human samples. Cited in 3 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human TRPM4.

Key facts

Isotype
IgG1
Form
Liquid
Clonality
Monoclonal
Immunogen
  • Recombinant Full Length Protein corresponding to Human TRPM4. Database link Q8TD43
Clone number
OTI10H5
Purification technique
Affinity purification
Concentration
Loading...
Purification notes

Purified from cell culture supernatant by affinity chromatography.

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

Clone OTI10H5 (formerly 10H5).

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

TRPM4 also known as Transient Receptor Potential Melastatin 4 is a calcium-activated non-selective cation channel with a mass of approximately 138 kilodaltons. This protein allows the movement of monovalent cations such as sodium across the plasma membrane leading to membrane depolarization. TRPM4 expresses in numerous human tissues including the heart brain and immune cells. Researchers often study this channel to understand its role in cellular electric activities.

Biological function summary

TRPM4 plays an important part in regulating membrane potential and cellular excitability. It does not function as part of a larger structural complex but its activity modulates various cellular processes. For instance it is important in cardiac conduction influencing heart rhythm by affecting the action potential duration. In immune cells TRPM4 contributes to cytokine release and cell movement impacting the body’s inflammatory response.

Pathways

TRPM4's mechanism integrates into significant signaling pathways like the calcium signaling pathway and the cardiac conduction system. Within these pathways its function influences several key proteins. In the calcium signaling pathway TRPM4's activity can affect the function of calmodulin an important calcium sensor while in cardiac cells TRPM4 regulates voltage-gated calcium channels important for heart muscle contraction.

Associated diseases and disorders

TRPM4’s roles associate it with conditions like cardiac arrhythmias and multiple sclerosis. In heart diseases TRPM4 interacts with proteins such as Na+/Ca2+ exchangers and abnormalities in this interaction can lead to arrhythmogenic conditions. For multiple sclerosis studies suggest that altered TRPM4 activity in immune cells impacts the immune response potentially linking it to the disease's progression. Understanding these interactions can provide insights for therapeutic targets.

Product promise

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In the unlikely event of one of our products not working as expected, you are covered by our product promise.

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7 product images

  • Western blot - Anti-TRPM4 antibody [OTI10H5] (ab123936), expandable thumbnail

    Western blot - Anti-TRPM4 antibody [OTI10H5] (ab123936)

    All lanes: Western blot - Anti-TRPM4 antibody [OTI10H5] (ab123936) at 1/5000 dilution

    Lane 1: HepG2 (Human liver hepatocellular carcinoma cell line) cell extract at 35 µg

    Lane 2: HeLa (Human epithelial cell line from cervix adenocarcinoma) cell extract at 35 µg

    Lane 3: HT-29 (Human colorectal adenocarcinoma cell line) cell extract at 35 µg

    Lane 4: A549 (Human lung carcinoma cell line) cell extract at 35 µg

    Lane 5: COS-7 (African green monkey kidney fibroblast-like cell line) cell extract at 35 µg

    Lane 6: Jurkat (Human T cell leukemia cell line from peripheral blood) cell extract at 35 µg

    Lane 7: MDCK (Canine kidney cell line) cell extract at 35 µg

    Lane 8: PC-12 (Rat adrenal gland pheochromocytoma cell line) cell extract at 35 µg

    Lane 9: MCF7 (Human breast adenocarcinoma cell line) cell extract at 35 µg

    Predicted band size: 134 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRPM4 antibody [OTI10H5] (ab123936), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRPM4 antibody [OTI10H5] (ab123936)

    Paraffin-embedded human kidney tissue stained for TRPM4 with ab123936 at a 1/50 dilution in immunohistochemical analysis. Heat-induced epitope retrieval by 10 mM citric buffer, pH 6.0, 100°C for 10 minutes.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRPM4 antibody [OTI10H5] (ab123936), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRPM4 antibody [OTI10H5] (ab123936)

    Paraffin-embedded human liver tissue stained for TRPM4 with ab123936 at a 1/50 dilution in immunohistochemical analysis. Heat-induced epitope retrieval by 10 mM citric buffer, pH 6.0, 100°C for 10 minutes.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRPM4 antibody [OTI10H5] (ab123936), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRPM4 antibody [OTI10H5] (ab123936)

    Paraffin-embedded human liver carcinoma tissue stained for TRPM4 with ab123936 at a 1/50 dilution in immunohistochemical analysis. Heat-induced epitope retrieval by 10 mM citric buffer, pH 6.0, 100°C for 10 minutes.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRPM4 antibody [OTI10H5] (ab123936), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRPM4 antibody [OTI10H5] (ab123936)

    Paraffin-embedded human ovary tissue stained for TRPM4 with ab123936 at a 1/50 dilution in immunohistochemical analysis. Heat-induced epitope retrieval by 10 mM citric buffer, pH 6.0, 100°C for 10 minutes.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRPM4 antibody [OTI10H5] (ab123936), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRPM4 antibody [OTI10H5] (ab123936)

    Paraffin-embedded human pancreas tissue stained for TRPM4 with ab123936 at a 1/50 dilution in immunohistochemical analysis. Heat-induced epitope retrieval by 10 mM citric buffer, pH 6.0, 100°C for 10 minutes.

  • Western blot - Anti-TRPM4 antibody [OTI10H5] (ab123936), expandable thumbnail

    Western blot - Anti-TRPM4 antibody [OTI10H5] (ab123936)

    All lanes: Western blot - Anti-TRPM4 antibody [OTI10H5] (ab123936) at 1/5000 dilution

    Lane 1: HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) cells transfected with pCMV6-ENTRY control cDNA at 5 µg

    Lane 2: HEK-293T cells transfected with pCMV6-ENTRY TRPM4 cDNA at 5 µg

    Predicted band size: 134 kDa

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Product protocols

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