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AB123936

Anti-TRPM4 antibody [OTI10H5]

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(5 Publications)

Mouse Monoclonal TRPM4 antibody. Suitable for IHC-P, WB and reacts with Human, Dog, African green monkey, Transfected cell lysate - Human samples. Cited in 5 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human TRPM4.

View Alternative Names

LTRPC4, TRPM4, Transient receptor potential cation channel subfamily M member 4, hTRPM4, Calcium-activated non-selective cation channel 1, Long transient receptor potential channel 4, Melastatin-4, LTrpC-4, LTrpC4

7 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRPM4 antibody [OTI10H5] (AB123936)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRPM4 antibody [OTI10H5] (AB123936)

Paraffin-embedded human pancreas tissue stained for TRPM4 with ab123936 at a 1/50 dilution in immunohistochemical analysis. Heat-induced epitope retrieval by 10 mM citric buffer, pH 6.0, 100°C for 10 minutes.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRPM4 antibody [OTI10H5] (AB123936)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRPM4 antibody [OTI10H5] (AB123936)

Paraffin-embedded human liver tissue stained for TRPM4 with ab123936 at a 1/50 dilution in immunohistochemical analysis. Heat-induced epitope retrieval by 10 mM citric buffer, pH 6.0, 100°C for 10 minutes.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRPM4 antibody [OTI10H5] (AB123936)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRPM4 antibody [OTI10H5] (AB123936)

Paraffin-embedded human kidney tissue stained for TRPM4 with ab123936 at a 1/50 dilution in immunohistochemical analysis. Heat-induced epitope retrieval by 10 mM citric buffer, pH 6.0, 100°C for 10 minutes.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRPM4 antibody [OTI10H5] (AB123936)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRPM4 antibody [OTI10H5] (AB123936)

Paraffin-embedded human ovary tissue stained for TRPM4 with ab123936 at a 1/50 dilution in immunohistochemical analysis. Heat-induced epitope retrieval by 10 mM citric buffer, pH 6.0, 100°C for 10 minutes.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRPM4 antibody [OTI10H5] (AB123936)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRPM4 antibody [OTI10H5] (AB123936)

Paraffin-embedded human liver carcinoma tissue stained for TRPM4 with ab123936 at a 1/50 dilution in immunohistochemical analysis. Heat-induced epitope retrieval by 10 mM citric buffer, pH 6.0, 100°C for 10 minutes.

Western blot - Anti-TRPM4 antibody [OTI10H5] (AB123936)
  • WB

Unknown

Western blot - Anti-TRPM4 antibody [OTI10H5] (AB123936)

All lanes:

Western blot - Anti-TRPM4 antibody [OTI10H5] (ab123936) at 1/5000 dilution

Lane 1:

HepG2 (Human liver hepatocellular carcinoma cell line) cell extract at 35 µg

Lane 2:

HeLa (Human epithelial cell line from cervix adenocarcinoma) cell extract at 35 µg

Lane 3:

HT-29 (Human colorectal adenocarcinoma cell line) cell extract at 35 µg

Lane 4:

A549 (Human lung carcinoma cell line) cell extract at 35 µg

Lane 5:

COS-7 (African green monkey kidney fibroblast-like cell line) cell extract at 35 µg

Lane 6:

Jurkat (Human T cell leukemia cell line from peripheral blood) cell extract at 35 µg

Lane 7:

MDCK (Canine kidney cell line) cell extract at 35 µg

Lane 8:

PC-12 (Rat adrenal gland pheochromocytoma cell line) cell extract at 35 µg

Lane 9:

MCF7 (Human breast adenocarcinoma cell line) cell extract at 35 µg

Predicted band size: 134 kDa

false

Western blot - Anti-TRPM4 antibody [OTI10H5] (AB123936)
  • WB

Unknown

Western blot - Anti-TRPM4 antibody [OTI10H5] (AB123936)

All lanes:

Western blot - Anti-TRPM4 antibody [OTI10H5] (ab123936) at 1/5000 dilution

Lane 1:

HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) cells transfected with pCMV6-ENTRY control cDNA at 5 µg

Lane 2:

HEK-293T cells transfected with pCMV6-ENTRY TRPM4 cDNA at 5 µg

Predicted band size: 134 kDa

false

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

OTI10H5

Isotype

IgG1

Carrier free

No

Reacts with

Dog, Human, African green monkey

Applications

WB, IHC-P

applications

Immunogen

Recombinant Full Length Protein corresponding to Human TRPM4.

Q8TD43

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/5000 - 1/10000", "WB-species-notes": "<p></p>" }, "African green monkey": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/5000 - 1/10000", "WB-species-notes": "<p></p>" }, "Dog": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/5000 - 1/10000", "WB-species-notes": "<p></p>" }, "Transfected cell lysate - Human": { "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/5000 - 1/10000", "WB-species-notes": "<p></p>" } } }
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Product details

Clone OTI10H5 (formerly 10H5).
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification
Purification notes
Purified from cell culture supernatant by affinity chromatography.
Storage buffer
pH: 7.3 Preservative: 0.02% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine), 1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

TRPM4 also known as Transient Receptor Potential Melastatin 4 is a calcium-activated non-selective cation channel with a mass of approximately 138 kilodaltons. This protein allows the movement of monovalent cations such as sodium across the plasma membrane leading to membrane depolarization. TRPM4 expresses in numerous human tissues including the heart brain and immune cells. Researchers often study this channel to understand its role in cellular electric activities.
Biological function summary

TRPM4 plays an important part in regulating membrane potential and cellular excitability. It does not function as part of a larger structural complex but its activity modulates various cellular processes. For instance it is important in cardiac conduction influencing heart rhythm by affecting the action potential duration. In immune cells TRPM4 contributes to cytokine release and cell movement impacting the body’s inflammatory response.

Pathways

TRPM4's mechanism integrates into significant signaling pathways like the calcium signaling pathway and the cardiac conduction system. Within these pathways its function influences several key proteins. In the calcium signaling pathway TRPM4's activity can affect the function of calmodulin an important calcium sensor while in cardiac cells TRPM4 regulates voltage-gated calcium channels important for heart muscle contraction.

TRPM4’s roles associate it with conditions like cardiac arrhythmias and multiple sclerosis. In heart diseases TRPM4 interacts with proteins such as Na+/Ca2+ exchangers and abnormalities in this interaction can lead to arrhythmogenic conditions. For multiple sclerosis studies suggest that altered TRPM4 activity in immune cells impacts the immune response potentially linking it to the disease's progression. Understanding these interactions can provide insights for therapeutic targets.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Calcium-activated selective cation channel that mediates membrane depolarization (PubMed : 12015988, PubMed : 12842017, PubMed : 29211723, PubMed : 30528822). While it is activated by increase in intracellular Ca(2+), it is impermeable to it (PubMed : 12015988). Mediates transport of monovalent cations (Na(+) > K(+) > Cs(+) > Li(+)), leading to depolarize the membrane (PubMed : 12015988). It thereby plays a central role in cadiomyocytes, neurons from entorhinal cortex, dorsal root and vomeronasal neurons, endocrine pancreas cells, kidney epithelial cells, cochlea hair cells etc. Participates in T-cell activation by modulating Ca(2+) oscillations after T lymphocyte activation, which is required for NFAT-dependent IL2 production. Involved in myogenic constriction of cerebral arteries. Controls insulin secretion in pancreatic beta-cells. May also be involved in pacemaking or could cause irregular electrical activity under conditions of Ca(2+) overload. Affects T-helper 1 (Th1) and T-helper 2 (Th2) cell motility and cytokine production through differential regulation of calcium signaling and NFATC1 localization. Enhances cell proliferation through up-regulation of the beta-catenin signaling pathway. Plays a role in keratinocyte differentiation (PubMed : 30528822).. Isoform 2. Lacks channel activity.
See full target information TRPM4
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Publications (5)

Recent publications for all applications. Explore the full list and refine your search

International journal of molecular sciences 25: PubMed39201277

2024

CHD1L Regulates Cell Survival in Breast Cancer and Its Inhibition by OTI-611 Impedes the DNA Damage Response and Induces PARthanatos.

Applications

Unspecified application

Species

Unspecified reactive species

Rita Sala,Hector Esquer,Timothy Kellett,Jeffrey T Kearns,Paul Awolade,Qiong Zhou,Daniel V LaBarbera

Cancer research communications 4:2101-2111 PubMed39041239

2024

Acetalax (Oxyphenisatin Acetate, NSC 59687) and Bisacodyl Cause Oncosis in Triple-Negative Breast Cancer Cell Lines by Poisoning the Ion Exchange Membrane Protein TRPM4.

Applications

Unspecified application

Species

Unspecified reactive species

Makito Mizunuma,Christophe E Redon,Liton Kumar Saha,Andy D Tran,Anjali Dhall,Robin Sebastian,Daiki Taniyama,Michael J Kruhlak,William C Reinhold,Naoko Takebe,Yves Pommier

Neuron 110:1944-1958.e8 PubMed35421327

2022

Functional coupling of TRPM2 and extrasynaptic NMDARs exacerbates excitotoxicity in ischemic brain injury.

Applications

Unspecified application

Species

Unspecified reactive species

Pengyu Zong,Jianlin Feng,Zhichao Yue,Yunfeng Li,Gongxiong Wu,Baonan Sun,Yanlin He,Barbara Miller,Albert S Yu,Zhongping Su,Jia Xie,Yasuo Mori,Bing Hao,Lixia Yue

Cancers 14: PubMed35158942

2022

Germline Variants of and Predispose to Familial Colorectal Cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Lizhen Zhu,Beiping Miao,Dagmara Dymerska,Magdalena Kuswik,Elena Bueno-Martínez,Lara Sanoguera-Miralles,Eladio A Velasco,Nagarajan Paramasivam,Matthias Schlesner,Abhishek Kumar,Ying Yuan,Jan Lubinski,Obul Reddy Bandapalli,Kari Hemminki,Asta Försti

Pflugers Archiv : European journal of physiology 473:521-531 PubMed33594499

2021

Upregulation of transient receptor potential melastatin 4 (TRPM4) in ventricular fibroblasts from heart failure patients.

Applications

Unspecified application

Species

Unspecified reactive species

Jianlin Feng,Pengyu Zong,Jiajie Yan,Zhichao Yue,Xin Li,Chevaughn Smith,Xun Ai,Lixia Yue
View all publications
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Product promise

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