Rabbit Polyclonal TRPM7 antibody. Suitable for WB, IHC-P and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Synthetic Peptide within Human TRPM7.
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- Scientific reports 13:224852023LncRNA SNHG8 regulates the migration and angiogenesis of pHUVECs induced by high glucose via the TRPM7/ERK signaling axis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesZongcheng Fan,Xin Chen,Laicheng Wang,Jianjian Yu,Shunpeng Zhang,Changsheng Xu,Jinxiu Lin,Yunchai Lin,Feng PengPubMed 38110485
- International journal of molecular sciences 22:2021TRPM7-Mediated Calcium Transport in HAT-7 Ameloblasts.Applications:Unspecified applicationReactive species:Unspecified reactive speciesKristóf Kádár,Viktória Juhász,Anna Földes,Róbert Rácz,Yan Zhang,Heike Löchli,Erzsébet Kató,László Köles,Martin C Steward,Pamela DenBesten,Gábor Varga,Ákos ZsemberyPubMed 33924361
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 20% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Synthetic Peptide within Human TRPM7. The exact immunogen used to generate this antibody is proprietary information. Database link Q96QT4
Reactivity data
Select an application| WB | IHC-P | |
|---|---|---|
| Human | Tested | Tested |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/500.00000 - 1/3000.00000 | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/100.00000 - 1/1000.00000 | Notes - |
Associated Products
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Target data
Function
Bifunctional protein that combines an ion channel with an intrinsic kinase domain, enabling it to modulate cellular functions either by conducting ions through the pore or by phosphorylating downstream proteins via its kinase domain. The channel is highly permeable to divalent cations, specifically calcium (Ca2+), magnesium (Mg2+) and zinc (Zn2+) and mediates their influx (PubMed:11385574, PubMed:12887921, PubMed:15485879, PubMed:24316671, PubMed:35561741, PubMed:36027648). Controls a wide range of biological processes such as Ca2(+), Mg(2+) and Zn(2+) homeostasis, vesicular Zn(2+) release channel and intracellular Ca(2+) signaling, embryonic development, immune responses, cell motility, proliferation and differentiation (By similarity). The C-terminal alpha-kinase domain autophosphorylates cytoplasmic residues of TRPM7 (PubMed:18365021). In vivo, TRPM7 phosphorylates SMAD2, suggesting that TRPM7 kinase may play a role in activating SMAD signaling pathways. In vitro, TRPM7 kinase phosphorylates ANXA1 (annexin A1), myosin II isoforms and a variety of proteins with diverse cellular functions (PubMed:15485879, PubMed:18394644). TRPM7 channel, cleaved form. The cleaved channel exhibits substantially higher current and potentiates Fas receptor signaling. TRPM7 kinase, cleaved form. The C-terminal kinase domain can be cleaved from the channel segment in a cell-type-specific fashion. In immune cells, the TRPM7 kinase domain is clipped from the channel domain by caspases in response to Fas-receptor stimulation. The cleaved kinase fragments can translocate to the nucleus, and bind chromatin-remodeling complex proteins in a Zn(2+)-dependent manner to ultimately phosphorylate specific Ser/Thr residues of histones known to be functionally important for cell differentiation and embryonic development.
Alternative names
CHAK1, LTRPC7, TRPM7, Transient receptor potential cation channel subfamily M member 7, Channel-kinase 1, Long transient receptor potential channel 7, LTrpC-7, LTrpC7
Recommended products
Rabbit Polyclonal TRPM7 antibody. Suitable for WB, IHC-P and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Synthetic Peptide within Human TRPM7.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 20% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Synthetic Peptide within Human TRPM7. The exact immunogen used to generate this antibody is proprietary information. Database link Q96QT4
- Purification technique
- Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
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Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
TRPM7 also known as transient receptor potential melastatin 7 is an ion channel and a kinase with dual functionality. This protein has a mass of about 190 kDa and is widely expressed in a variety of tissues including brain heart kidney and lung. Mechanically TRPM7 serves as a channel for divalent cations primarily allowing the movement of magnesium (Mg²⁺) and calcium (Ca²⁺) ions across cellular membranes. Its kinase domain plays a role in autophosphorylation and substrate phosphorylation further influencing cellular processes.
- Biological function summary
TRPM7 regulates cellular magnesium homeostasis and calcium influx in many cell types. It is integral in maintaining the cell's ion balance and influences various cellular functions like growth proliferation and apoptosis. TRPM7 is often part of larger molecular complexes that enable it to interact with other proteins and lipids modulating signal transduction pathways. It participates in mechanosensory functions as well impacting cellular responses to mechanical stimuli.
- Pathways
TRPM7 is significant in the MAPK and PI3K/Akt pathways which are pivotal for cell survival and proliferation. Through these pathways TRPM7 interacts with other proteins such as TRPM6 and TRPV4. It modulates intracellular signaling cascades by regulating ion concentrations that affect protein interactions and enzyme activities thereby controlling critical cellular responses like inflammation and differentiation.
- Associated diseases and disorders
TRPM7 is linked to cancer and neurodegenerative diseases. In cancers altered TRPM7 expression promotes tumor growth and metastasis potentially interacting with proteins like Akt. In neurodegenerative conditions such as Alzheimer's disease abnormal TRPM7 function or expression contributes to neuronal death and is associated with other proteins like amyloid-beta and tau making TRPM7 a potential therapeutic target for modulation.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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2 product images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRPM7 antibody (ab262698)
Immunohistochemical analysis of paraffin-embedded human liver tissue labeling TRPM7 with ab262698 at 1/250 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15mins.
Western blot - Anti-TRPM7 antibody (ab262698)
All lanes: Western blot - Anti-TRPM7 antibody (ab262698) at 1/1000 dilution
All lanes: HCT116 whole cell lysate at 30 µg
Predicted band size: 212 kDa
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