Rabbit Recombinant Monoclonal TRPM6 antibody. Carrier free. Suitable for WB and reacts with Human samples. Cited in 4 publications.
IgG
Rabbit
pH: 7.2 - 7.4
Constituents: PBS
Liquid
Monoclonal
WB | |
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Human | Tested |
Species | Dilution info | Notes |
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Species Human | Dilution info - | Notes - |
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Bifunctional protein that combines an ion channel with an intrinsic kinase domain, enabling it to modulate cellular functions either by conducting ions through the pore or by phosphorylating downstream proteins via its kinase domain (PubMed:14576148, PubMed:16636202, PubMed:18258429, PubMed:18365021). Crucial for Mg(2+) homeostasis. Has an important role in epithelial Mg(2+) transport and in the active Mg(2+) absorption in the gut and kidney (PubMed:14576148). However, whether TRPM6 forms functional homomeric channels by itself or functions primarily as a subunit of heteromeric TRPM6-TRPM7 channels, is still under debate (PubMed:14576148, PubMed:16636202, PubMed:24385424).TRPM6 kinase, cleaved formThe C-terminal kinase domain can be cleaved from the channel segment in a cell-type-specific fashion. The cleaved kinase fragments can translocate to the nucleus, and bind chromatin-remodeling complex proteins to ultimately phosphorylate specific Ser/Thr residues of histones known to be functionally important for cell differentiation and development.
TRPM7
CHAK2, CHAK2, TRPM6, Transient receptor potential cation channel subfamily M member 6, Channel kinase 2, Melastatin-related TRP cation channel 6
Rabbit Recombinant Monoclonal TRPM6 antibody. Carrier free. Suitable for WB and reacts with Human samples. Cited in 4 publications.
IgG
Rabbit
pH: 7.2 - 7.4
Constituents: PBS
Liquid
Monoclonal
Yes
EPR4582
Affinity purification Protein A
The immunogen used for this product shares 86% homology with TRPM6 (ten amino acid stretch with 100% homology). ab109438 (The same clone in a diffrent buffer) has been shown to cross react with TRPM6 in WB.
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ab232455 is the carrier-free version of Anti-TRPM7 + TRPM6 antibody [EPR4582] ab109438.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
This supplementary information is collated from multiple sources and compiled automatically.
TRPM7 and TRPM6 are members of the transient receptor potential melastatin subfamily often referred to as TRP-PLIK or CHAK1 for TRPM7. TRPM7 has a molecular mass of approximately 165 kDa while TRPM6 is roughly 230 kDa. These proteins function as ion channels with kinase activity. They regulate the homeostasis of divalent cations like magnesium and calcium by permitting their entry into cells. TRPM7 is expressed in various tissues including the heart liver and brain while TRPM6 is mostly found in the kidney and intestine.
TRPM7 and TRPM6 play essential roles in cellular magnesium absorption and homeostasis. They form homo- or heteromeric channel complexes with the combination of TRPM6 and TRPM7 enhancing channel function. These proteins influence critical cellular processes such as proliferation apoptosis and migration by modulating cellular magnesium levels. TRPM7's distinct kinase domain also impacts intracellular signaling pathways through phosphorylation of specific substrates regulating response to cellular stress and differentiation.
TRPM7 and TRPM6 modulate cell signaling pathways linked to magnesium-sensitive enzymes and proteins like those involved in the PI3K/Akt signaling pathway. This pathway is integral for cell growth survival and metabolism. TRPM7 interacts with various signaling proteins including PLC and MAPK influencing cellular responses to external stimuli. TRPM6 often partners with TRPM7 to fine-tune these cellular pathways maintaining appropriate ionic balances necessary for cellular functions.
TRPM7 and TRPM6 are linked to magnesium homeostasis-related conditions such as hypomagnesemia and hypertension. Variants in TRPM6 associate with hypomagnesemia with secondary hypocalcemia a genetic disorder affecting magnesium uptake in the intestine. TRPM7 is connected to neurodegenerative diseases given its role in neuronal survival and response to oxidative stress. The interaction of TRPM7 with proteins involved in neurodegenerative pathways like amyloid precursor protein suggests potential involvement in Alzheimer’s disease pathogenesis.
We have tested this species and application combination and it works. It is covered by our product promise.
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
We do not recommend this combination. It is not covered by our product promise.
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Terms & Conditions.
Blocking and diluting buffer: 5% NFDM/TBST.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-TRPM7 + TRPM6 antibody [EPR4582] ab109438).
All lanes: Western blot - Anti-TRPM7 + TRPM6 antibody [EPR4582] (Anti-TRPM7 + TRPM6 antibody [EPR4582] ab109438) at 1/1000 dilution
All lanes: JAR (Human placenta choriocarcinoma epithelial cell) whole cell lysates at 15 µg
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution
Predicted band size: 49 kDa
Observed band size: 27 kDa
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