AB232455

Anti-TRPM7 + TRPM6 antibody [EPR4582] - BSA and Azide free

RabMAb
Recombinant
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(5 Publications)

Rabbit Recombinant Monoclonal TRPM6 antibody. Carrier free. Suitable for WB and reacts with Human samples. Cited in 5 publications.

View Alternative Names

CHAK2, TRPM6, Transient receptor potential cation channel subfamily M member 6, Channel kinase 2, Melastatin-related TRP cation channel 6

1 Images

Lab

Western blot - Anti-TRPM7 + TRPM6 antibody [EPR4582] - BSA and Azide free (AB232455)

Blocking and diluting buffer : 5% NFDM/TBST.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab109438).

All lanes:

Western blot - Anti-TRPM7 + TRPM6 antibody [EPR4582] (<a href='/en-us/products/primary-antibodies/trpm7-trpm6-antibody-epr4582-ab109438'>ab109438</a>) at 1/1000 dilution

All lanes:

JAR (Human placenta choriocarcinoma epithelial cell) whole cell lysates at 15 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 49 kDa

Observed band size: 27 kDa

false

Unconjugated

Anti-TRPM7 + TRPM6 antibody [EPR4582]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR4582

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Specificity

The immunogen used for this product shares 86% homology with TRPM6 (ten amino acid stretch with 100% homology). ab109438 (The same clone in a diffrent buffer) has been shown to cross react with TRPM6 in WB.

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Reactivity data

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Product details

ab232455 is the carrier-free version of ab109438.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

Do Not Freeze

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

TRPM7 and TRPM6 are members of the transient receptor potential melastatin subfamily often referred to as TRP-PLIK or CHAK1 for TRPM7. TRPM7 has a molecular mass of approximately 165 kDa while TRPM6 is roughly 230 kDa. These proteins function as ion channels with kinase activity. They regulate the homeostasis of divalent cations like magnesium and calcium by permitting their entry into cells. TRPM7 is expressed in various tissues including the heart liver and brain while TRPM6 is mostly found in the kidney and intestine.

Biological function summary

TRPM7 and TRPM6 play essential roles in cellular magnesium absorption and homeostasis. They form homo- or heteromeric channel complexes with the combination of TRPM6 and TRPM7 enhancing channel function. These proteins influence critical cellular processes such as proliferation apoptosis and migration by modulating cellular magnesium levels. TRPM7's distinct kinase domain also impacts intracellular signaling pathways through phosphorylation of specific substrates regulating response to cellular stress and differentiation.

Pathways

TRPM7 and TRPM6 modulate cell signaling pathways linked to magnesium-sensitive enzymes and proteins like those involved in the PI3K/Akt signaling pathway. This pathway is integral for cell growth survival and metabolism. TRPM7 interacts with various signaling proteins including PLC and MAPK influencing cellular responses to external stimuli. TRPM6 often partners with TRPM7 to fine-tune these cellular pathways maintaining appropriate ionic balances necessary for cellular functions.

TRPM7 and TRPM6 are linked to magnesium homeostasis-related conditions such as hypomagnesemia and hypertension. Variants in TRPM6 associate with hypomagnesemia with secondary hypocalcemia a genetic disorder affecting magnesium uptake in the intestine. TRPM7 is connected to neurodegenerative diseases given its role in neuronal survival and response to oxidative stress. The interaction of TRPM7 with proteins involved in neurodegenerative pathways like amyloid precursor protein suggests potential involvement in Alzheimer’s disease pathogenesis.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Bifunctional protein that combines an ion channel with an intrinsic kinase domain, enabling it to modulate cellular functions either by conducting ions through the pore or by phosphorylating downstream proteins via its kinase domain (PubMed : 14576148, PubMed : 16636202, PubMed : 18258429, PubMed : 18365021). Crucial for Mg(2+) homeostasis. Has an important role in epithelial Mg(2+) transport and in the active Mg(2+) absorption in the gut and kidney (PubMed : 14576148). However, whether TRPM6 forms functional homomeric channels by itself or functions primarily as a subunit of heteromeric TRPM6-TRPM7 channels, is still under debate (PubMed : 14576148, PubMed : 16636202, PubMed : 24385424).. TRPM6 kinase, cleaved form. The C-terminal kinase domain can be cleaved from the channel segment in a cell-type-specific fashion. The cleaved kinase fragments can translocate to the nucleus, and bind chromatin-remodeling complex proteins to ultimately phosphorylate specific Ser/Thr residues of histones known to be functionally important for cell differentiation and development.

See full target information TRPM6

Additional targets

TRPM7

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Publications (5)

Recent publications for all applications. Explore the full list and refine your search

CNS neuroscience & therapeutics 30:e70123 PubMed39564756

2024

Lycium barbarum Extract Enhanced Neuroplasticity and Functional Recovery in 5xFAD Mice via Modulating Microglial Status of the Central Nervous System.

Applications

Unspecified application

Species

Unspecified reactive species

Zhongqing Sun,Jinfeng Liu,Zihang Chen,Kwok-Fai So,Yong Hu,Kin Chiu

Cellular signalling 94:110306 PubMed35292358

2022

LncRNA HOTAIR sponges miR-301a-3p to promote glioblastoma proliferation and invasion through upregulating FOSL1.

Applications

Unspecified application

Species

Unspecified reactive species

Shanchun Guo,Pendelton King,Emily Liang,Alyssa A Guo,Mingli Liu

Theranostics 11:7450-7470 PubMed34158860

2021

Inhibition of SENP6 restrains cerebral ischemia-reperfusion injury by regulating Annexin-A1 nuclear translocation-associated neuronal apoptosis.

Applications

Unspecified application

Species

Unspecified reactive species

Qian Xia,Meng Mao,Zhen Zeng,Zhenzhao Luo,Yin Zhao,Jing Shi,Xing Li

Frontiers in pharmacology 11:590723 PubMed33381038

2020

TRPM7 Induces Tumorigenesis and Stemness Through Notch Activation in Glioma.

Applications

Unspecified application

Species

Unspecified reactive species

Jingwei Wan,Alyssa Aihui Guo,Pendelton King,Shanchun Guo,Talib Saafir,Yugang Jiang,Mingli Liu

Frontiers in oncology 9:1413 PubMed31921670

2019

TRPM7 Induces Mechanistic Target of Rap1b Through the Downregulation of miR-28-5p in Glioma Proliferation and Invasion.

Applications

Unspecified application

Species

Unspecified reactive species

Jingwei Wan,Alyssa Aihui Guo,Indrajit Chowdhury,Shanchun Guo,Jacqueline Hibbert,Guangdi Wang,Mingli Liu

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-TRPM7 + TRPM6 antibody [EPR4582] - BSA and Azide free

Western blot - Anti-TRPM7 + TRPM6 antibody [EPR4582] - BSA and Azide free (AB232455)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Specificity

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Additional targets

Publications (5)

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Product promise