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Rabbit Recombinant Monoclonal TRPM8 antibody. Suitable for IHC-P and reacts with Human samples.

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Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRPM8 antibody [2G7] (AB314169), expandable thumbnail

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), 49.1% PBS, 0.88% Sodium chloride

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IHC-P
Human
Tested

Tested
Tested

Species
Human
Dilution info
1/50.00000 - 1/200.00000
Notes

-

Target data

Function

Non-selective ion channel permeable to monovalent and divalent cations, including Na(+), K(+), and Ca(2+), with higher permeability for Ca(2+). Activated by multiple factors, such as temperature, voltage, pressure, and changes in osmolality. Activated by cool temperatures (<23-28 degrees Celsius) and by chemical ligands evoking a sensation of coolness, such as menthol and icilin therefore plays a central role in the detection of environmental cold temperatures (PubMed:15306801, PubMed:15852009, PubMed:16174775, PubMed:25559186, PubMed:37857704). TRPM8 is a voltage-dependent channel; its activation by cold or chemical ligands shifts its voltage thresholds towards physiological membrane potentials, leading to the opening of the channel (PubMed:15306801). In addition to its critical role in temperature sensing, regulates basal tear secretion by sensing evaporation-induced cooling and changes in osmolality (By similarity). May plays a role in prostate cancer cell migration (PubMed:16174775, PubMed:25559186). Isoform 2. Negatively regulates menthol- and cold-induced channel activity by stabilizing the closed state of the channel. Isoform 3. Negatively regulates menthol- and cold-induced channel activity by stabilizing the closed state of the channel.

Alternative names

LTRPC6, TRPP8, TRPM8, Transient receptor potential cation channel subfamily M member 8, Long transient receptor potential channel 6, Transient receptor potential p8, LTrpC-6, LTrpC6, Trp-p8

Recommended products

Rabbit Recombinant Monoclonal TRPM8 antibody. Suitable for IHC-P and reacts with Human samples.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
2G7
Purification technique
Affinity purification
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

TRPM8 also known as Transient Receptor Potential Cation Channel Subfamily M Member 8 or Menthol Receptor is a cold-activated ion channel. TRPM8 has a molecular weight of approximately 128 kDa. This protein mainly localizes on the plasma membrane of sensory neurons within the dorsal root and trigeminal ganglia. It plays a mechanical role by facilitating the influx of Ca2+ and Na+ ions leading to depolarization which contributes to cold sensation.

Biological function summary

TRPM8 functions as a critical sensor for detecting temperatures below 28°C and is activated by natural compounds like menthol and icilin. It does not form part of any known protein complexes but can interact with lipids in the cell membrane influencing its activity. It serves in thermosensation providing an immediate response to environmental temperature changes thereby playing an important role in maintaining body homeostasis.

Pathways

TRPM8 is integral to thermoregulatory pathways specifically involved in cold sensation. It participates in the pathway related to the TRP channel family which includes related channels like TRPV1 known for heat sensation. Another pathway is the nociceptive pathway where TRPM8 participates in transmitting signals associated with pain that is triggered by cold temperatures.

Associated diseases and disorders

TRPM8 has been linked to conditions such as neuropathic pain and prostate cancer. In neuropathic pain TRPM8 can exacerbate pain sensation due to its heightened activation. In prostate cancer elevated levels of TRPM8 expression have been observed connecting it to disease progression. The protein's relation to these disorders often associates it closely with other proteins like TRPV1 in pain mechanisms and androgen receptors in prostate cancer.

Product promise

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In the unlikely event of one of our products not working as expected, you are covered by our product promise.

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1 product image

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRPM8 antibody [2G7] (ab314169), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRPM8 antibody [2G7] (ab314169)

    Immunohistochemical analysis of ab314169 diluted at 1:100 and staining in paraffin-embedded human prostate cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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