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AB235890

Anti-TRPM8 antibody

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(1 Publication)

Rabbit Polyclonal TRPM8 antibody. Suitable for IHC-P, ICC/IF and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human TRPM8 aa 1-200.

View Alternative Names

LTRPC6, TRPP8, TRPM8, Transient receptor potential cation channel subfamily M member 8, Long transient receptor potential channel 6, Transient receptor potential p8, LTrpC-6, LTrpC6, Trp-p8

3 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRPM8 antibody (AB235890)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRPM8 antibody (AB235890)

Paraffin-embedded human lung cancer tissue stained for TRPM8 using ab235890 at 1/100 dilution in immunohistochemical analysis.

Immunocytochemistry/ Immunofluorescence - Anti-TRPM8 antibody (AB235890)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-TRPM8 antibody (AB235890)

A549 (Human lung carcinoma cell line) cells stained for TRPM8 (green) using ab235890 at 1/100 dilution in ICC/IF, followed by an Alexa-Fluor 488® conjugated Goat Anti-Rabbit IgG (H+L).

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRPM8 antibody (AB235890)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRPM8 antibody (AB235890)

Paraffin-embedded human lung tissue stained for TRPM8 using ab235890 at 1/100 dilution in immunohistochemical analysis.

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

IHC-P, ICC/IF

applications

Immunogen

Recombinant Fragment Protein within Human TRPM8 aa 1-200. The exact immunogen used to generate this antibody is proprietary information.

Q7Z2W7

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/20 - 1/200", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/50 - 1/200", "ICCIF-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein G
Purification notes
Purity >95%
Storage buffer
pH: 7.4 Preservative: 0.03% Proclin 300 Constituents: PBS, 50% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

TRPM8 also known as Transient Receptor Potential Cation Channel Subfamily M Member 8 or Menthol Receptor is a cold-activated ion channel. TRPM8 has a molecular weight of approximately 128 kDa. This protein mainly localizes on the plasma membrane of sensory neurons within the dorsal root and trigeminal ganglia. It plays a mechanical role by facilitating the influx of Ca2+ and Na+ ions leading to depolarization which contributes to cold sensation.
Biological function summary

TRPM8 functions as a critical sensor for detecting temperatures below 28°C and is activated by natural compounds like menthol and icilin. It does not form part of any known protein complexes but can interact with lipids in the cell membrane influencing its activity. It serves in thermosensation providing an immediate response to environmental temperature changes thereby playing an important role in maintaining body homeostasis.

Pathways

TRPM8 is integral to thermoregulatory pathways specifically involved in cold sensation. It participates in the pathway related to the TRP channel family which includes related channels like TRPV1 known for heat sensation. Another pathway is the nociceptive pathway where TRPM8 participates in transmitting signals associated with pain that is triggered by cold temperatures.

TRPM8 has been linked to conditions such as neuropathic pain and prostate cancer. In neuropathic pain TRPM8 can exacerbate pain sensation due to its heightened activation. In prostate cancer elevated levels of TRPM8 expression have been observed connecting it to disease progression. The protein's relation to these disorders often associates it closely with other proteins like TRPV1 in pain mechanisms and androgen receptors in prostate cancer.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Non-selective ion channel permeable to monovalent and divalent cations, including Na(+), K(+), and Ca(2+), with higher permeability for Ca(2+). Activated by multiple factors, such as temperature, voltage, pressure, and changes in osmolality. Activated by cool temperatures (<23-28 degrees Celsius) and by chemical ligands evoking a sensation of coolness, such as menthol and icilin therefore plays a central role in the detection of environmental cold temperatures (PubMed : 15306801, PubMed : 15852009, PubMed : 16174775, PubMed : 25559186, PubMed : 37857704). TRPM8 is a voltage-dependent channel; its activation by cold or chemical ligands shifts its voltage thresholds towards physiological membrane potentials, leading to the opening of the channel (PubMed : 15306801). In addition to its critical role in temperature sensing, regulates basal tear secretion by sensing evaporation-induced cooling and changes in osmolality (By similarity). May plays a role in prostate cancer cell migration (PubMed : 16174775, PubMed : 25559186).. Isoform 2. Negatively regulates menthol- and cold-induced channel activity by stabilizing the closed state of the channel.. Isoform 3. Negatively regulates menthol- and cold-induced channel activity by stabilizing the closed state of the channel.
See full target information TRPM8
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Nature communications 14:4796 PubMed37558705

2023

Single-cell analysis reveals region-heterogeneous responses in rhesus monkey spinal cord with complete injury.

Applications

Unspecified application

Species

Unspecified reactive species

Yongheng Fan,Xianming Wu,Sufang Han,Qi Zhang,Zheng Sun,Bing Chen,Xiaoyu Xue,Haipeng Zhang,Zhenni Chen,Man Yin,Zhifeng Xiao,Yannan Zhao,Jianwu Dai
View all publications
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Product promise

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