Rabbit Polyclonal TRPS1 antibody. Suitable for IP, WB, IHC-P and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human Zinc finger transcription factor Trps1 aa 1000-1050.
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Publications
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- Evidence-based complementary and alternative medicine : eCAM 2022:60199752022Paclitaxel Resistance Modulated by the Interaction between TRPS1 and AF178030.2 in Triple-Negative Breast Cancer.Applications:Unspecified applicationReactive species:Unspecified reactive speciesTao Zhao,Tingrong Zhang,Yao Zhang,Bin Zhou,Xiangdong LuPubMed 35399640
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7 - 8
Preservative: 0.09% Sodium azide
Constituents: 99% Tris citrate/phosphate- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Synthetic Peptide within Human Zinc finger transcription factor Trps1 aa 1000-1050. The exact immunogen used to generate this antibody is proprietary information. Database link Q9UHF7
Reactivity data
Select an application| IP | WB | IHC-P | |
|---|---|---|---|
| Human | Tested | Tested | Tested |
| Rat | Predicted | Predicted | Predicted |
| Chicken | Predicted | Predicted | Predicted |
| Chimpanzee | Predicted | Predicted | Predicted |
| Cow | Predicted | Predicted | Predicted |
| Dog | Predicted | Predicted | Predicted |
| Gorilla | Predicted | Predicted | Predicted |
| Guinea pig | Predicted | Predicted | Predicted |
| Horse | Predicted | Predicted | Predicted |
| Orangutan | Predicted | Predicted | Predicted |
| Pig | Predicted | Predicted | Predicted |
| Platypus | Predicted | Predicted | Predicted |
| Rabbit | Predicted | Predicted | Predicted |
| Rhesus monkey | Predicted | Predicted | Predicted |
| Turkey | Predicted | Predicted | Predicted |
IP
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 2.00000-10.00000 µg/mg of lysate | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Dog, Turkey, Pig, Chimpanzee, Rhesus monkey, Gorilla, Orangutan, Platypus | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/2000.00000 - 1/10000.00000 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Dog, Turkey, Pig, Chimpanzee, Rhesus monkey, Gorilla, Orangutan, Platypus | Dilution info - | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/500.00000 - 1/2000.00000 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Dog, Turkey, Pig, Chimpanzee, Rhesus monkey, Gorilla, Orangutan, Platypus | Dilution info - | Notes - |
Associated Products
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3 products for Alternative Product
Target data
Function
Transcriptional repressor. Binds specifically to GATA sequences and represses expression of GATA-regulated genes at selected sites and stages in vertebrate development. Regulates chondrocyte proliferation and differentiation. Executes multiple functions in proliferating chondrocytes, expanding the region of distal chondrocytes, activating proliferation in columnar cells and supporting the differentiation of columnar into hypertrophic chondrocytes.
Alternative names
Zinc finger transcription factor Trps1, Tricho-rhino-phalangeal syndrome type I protein, Zinc finger protein GC79, TRPS1
Recommended products
Rabbit Polyclonal TRPS1 antibody. Suitable for IP, WB, IHC-P and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human Zinc finger transcription factor Trps1 aa 1000-1050.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7 - 8
Preservative: 0.09% Sodium azide
Constituents: 99% Tris citrate/phosphate- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Synthetic Peptide within Human Zinc finger transcription factor Trps1 aa 1000-1050. The exact immunogen used to generate this antibody is proprietary information. Database link Q9UHF7
- Purification technique
- Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
TRPS1 also known as Trichorhinophalangeal syndrome type I protein is a transcriptional regulator with a molecular weight of approximately 141 kDa. It is expressed in a variety of tissues including bone cartilage and hair follicles. Mechanically TRPS1 functions by binding to specific DNA sequences and regulating the transcription of target genes. This gene encodes a protein that contains GATA-type zinc finger domains which facilitate its role in DNA binding and transcription regulation.
- Biological function summary
The TRPS1 protein plays a significant role in the development of bone and cartilage as well as the differentiation of hair follicles. It acts independently and does not commonly form a stable complex with other proteins. By influencing the expression of genes involved in these developmental processes TRPS1 contributes to various aspects of skeletal and hair follicle formation. The regulation by TRPS1 is important for the proper growth and maintenance of these tissues.
- Pathways
The protein TRPS1 is notably involved in bone morphogenesis and development pathways. It interacts with signaling proteins like BMPs (Bone Morphogenetic Proteins) within the bone development pathway to regulate bone and cartilage growth. TRPS1 also communicates with signaling pathways that include proteins such as RUNX2 which are critical for bone formation and regulate the transcriptional activity that drives these processes.
- Associated diseases and disorders
Dysregulation or mutation of the TRPS1 gene is closely linked to Trichorhinophalangeal Syndrome a condition characterized by distinctive craniofacial and skeletal abnormalities. This association involves altered gene expression impacting bone and cartilage tied to TRPS1's regulation of signaling networks with proteins like BMPR1A. The TRPS1 gene is also linked to certain forms of cancer where its altered function may contribute to tumorigenesis through pathways shared with cancer-related proteins such as SMAD4 influencing cell proliferation and apoptosis.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
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4 product images
Immunoprecipitation - Anti-TRPS1 antibody (ab125197)
ab125197 at 1 μg/ml staining TRPS1 by WB, following immunoprecipitation of whole cell lysate from HeLa cells.
Lane 1; IP using ab125197 at 6 μg/mg lysate.
Lane 2; IP using control IgG.
1 mg of lysate was used for IP and 20% of IP was loaded. Detection utilised Chemiluminescence with a 30 second exposure.All lanes: Immunoprecipitation - Anti-TRPS1 antibody (ab125197)
Predicted band size: 142 kDa
Western blot - Anti-TRPS1 antibody (ab125197)
All lanes: Western blot - Anti-TRPS1 antibody (ab125197) at 0.1 µg/mL
Lane 1: HeLa whole cell lysate at 50 µg
Lane 2: HeLa whole cell lysate at 15 µg
Lane 3: 293T whole cell lysate at 50 µg
Lane 4: Jurkat whole cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 142 kDa
Exposure time: 3min
This image is courtesy of an anonymous customer reviewWestern blot - Anti-TRPS1 antibody (ab125197)
All lanes: Western blot - Anti-TRPS1 antibody (ab125197) at 1/500 dilution
Lane 1: Ha-Ras-transformed EpH4 cells - nuclear extracts at 25 µg
Lanes 2 - 3: EpF-2 and EpF-1 (EpH4 subclones expressing high amounts of Zeb1 and Zeb2 mRNA) - nuclear extracts at 25 µg
Lane 4: EpH4 (parental) - nuclear extracts at 25 µg
Lane 5: EpF-2 - nuclear extracts at 25 µg
Lanes 6 - 7: EpF-Z1.1 and EpF-Z1.2 (EpF-1 clones transfected with siRNA to knockdown Zeb1) - nuclear extracts at 25 µg
Lanes 8 - 9: EpF-Z2.1 and EpF-Z2.2 (EpF-1 clones transfected with siRNA to knockdown Zeb2) - nuclear extracts at 25 µg
SecondaryAll lanes: HRP-conjugated donkey anti-rabbit IgG polyclonal at 1/10000 dilution
Performed under reducing conditions.
Predicted band size: 142 kDa
Exposure time: 20s
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRPS1 antibody (ab125197)
Paraffin embedded human breast carcinoma tissue stained for TRPS1 using ab125197 at 1/1000 dilution in immunohistochemical analysis.
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