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Anti-TRPS1 antibody [EPR16171] ab209664 is a rabbit monoclonal antibody that is used in TRPS1 IHC. Suitable for human samples.

- Recombinant format for unrivaled batch-batch consistency: no need for same-lot requests
- Antibody clone EPR16171 is the most widely used clone for TRPS1 on the market
- Specificity and sensitivity confirmed in IHC with multi-tissue microarray (TMA) validation

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Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRPS1 antibody [EPR16171] (AB209664), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRPS1 antibody [EPR16171] (AB209664), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRPS1 antibody [EPR16171] (AB209664), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRPS1 antibody [EPR16171] (AB209664), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRPS1 antibody [EPR16171] (AB209664), expandable thumbnail

Publications

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IHC-P
Human
Tested

Tested
Tested

Species
Human
Dilution info
1/8000
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

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Target data

Function

Transcriptional repressor. Binds specifically to GATA sequences and represses expression of GATA-regulated genes at selected sites and stages in vertebrate development. Regulates chondrocyte proliferation and differentiation. Executes multiple functions in proliferating chondrocytes, expanding the region of distal chondrocytes, activating proliferation in columnar cells and supporting the differentiation of columnar into hypertrophic chondrocytes.

Alternative names

Zinc finger transcription factor Trps1, Tricho-rhino-phalangeal syndrome type I protein, Zinc finger protein GC79, TRPS1

Recommended products

Anti-TRPS1 antibody [EPR16171] ab209664 is a rabbit monoclonal antibody that is used in TRPS1 IHC. Suitable for human samples.

- Recombinant format for unrivaled batch-batch consistency: no need for same-lot requests
- Antibody clone EPR16171 is the most widely used clone for TRPS1 on the market
- Specificity and sensitivity confirmed in IHC with multi-tissue microarray (TMA) validation

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
EPR16171
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

TRPS1 also known as Trichorhinophalangeal syndrome type I protein is a transcriptional regulator with a molecular weight of approximately 141 kDa. It is expressed in a variety of tissues including bone cartilage and hair follicles. Mechanically TRPS1 functions by binding to specific DNA sequences and regulating the transcription of target genes. This gene encodes a protein that contains GATA-type zinc finger domains which facilitate its role in DNA binding and transcription regulation.

Biological function summary

The TRPS1 protein plays a significant role in the development of bone and cartilage as well as the differentiation of hair follicles. It acts independently and does not commonly form a stable complex with other proteins. By influencing the expression of genes involved in these developmental processes TRPS1 contributes to various aspects of skeletal and hair follicle formation. The regulation by TRPS1 is important for the proper growth and maintenance of these tissues.

Pathways

The protein TRPS1 is notably involved in bone morphogenesis and development pathways. It interacts with signaling proteins like BMPs (Bone Morphogenetic Proteins) within the bone development pathway to regulate bone and cartilage growth. TRPS1 also communicates with signaling pathways that include proteins such as RUNX2 which are critical for bone formation and regulate the transcriptional activity that drives these processes.

Associated diseases and disorders

Dysregulation or mutation of the TRPS1 gene is closely linked to Trichorhinophalangeal Syndrome a condition characterized by distinctive craniofacial and skeletal abnormalities. This association involves altered gene expression impacting bone and cartilage tied to TRPS1's regulation of signaling networks with proteins like BMPR1A. The TRPS1 gene is also linked to certain forms of cancer where its altered function may contribute to tumorigenesis through pathways shared with cancer-related proteins such as SMAD4 influencing cell proliferation and apoptosis.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

6 product images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRPS1 antibody [EPR16171] (ab209664), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRPS1 antibody [EPR16171] (ab209664)

    Immunohistochemical analysis of paraffin-embedded human breast tissue labeling TRPS1 with ab209664 at 1/8000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/500 dilution. Nuclear staining on epithelium of human breast is observed. Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/500 dilution.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRPS1 antibody [EPR16171] (ab209664), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRPS1 antibody [EPR16171] (ab209664)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue labeling TRPS1 with ab209664 at 1/8000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/500 dilution. Nuclear staining on human breast cancer is observed. Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/500 dilution.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRPS1 antibody [EPR16171] (ab209664), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRPS1 antibody [EPR16171] (ab209664)

    Immunohistochemical analysis of paraffin-embedded human breast cancer (stage II, left ; stage III right) tissue labeling TRPS1 with ab209664 at 1/8000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/500 dilution.

    (Left): Nucleus staining in the cancer cells of breast cancer stage II clinical case; (Right): Negative staining in the breast cancer stage III clinical case; The high expression of TRPS1 may facilitate improved survival of this breast cancer stage II case.

    Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/500 dilution.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRPS1 antibody [EPR16171] (ab209664), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRPS1 antibody [EPR16171] (ab209664)

    Immunohistochemical analysis of paraffin-embedded human kidney tissue labeling TRPS1 with ab209664 at 1/8000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/500 dilution. Negative staining on the human kidney. Counter stained with Hematoxylin.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRPS1 antibody [EPR16171] (ab209664), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRPS1 antibody [EPR16171] (ab209664)

    Immunohistochemical analysis of Paraffin-embedded sections human breast (A), endometrium (B) and colon (C) tissue labelling TRPS1 with ab209664 at 1/18000 dilution, followed by a ready to use secondary Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101). Counter stained with Haematoxylin. Secondary antibody only control: Used PBS instead of primary antibody.

    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

    Positive staining on (A) human breast; weak staining on (B) human endometrium; no staining on (C) human colon. The section was incubated with ab209664 for 30 mins at room temperature.
    The immunostaining was performed on a Leica Biosystems BOND® RX instrument.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRPS1 antibody [EPR16171] (ab209664), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRPS1 antibody [EPR16171] (ab209664)

    Immunohistochemical analysis of Paraffin-embedded sections human breast cancer (A), endometrium cancer(B) and colon caner (C) tissue labelling TRPS1 with ab209664 at 1/18000 dilution, followed by a ready to use secondary Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101). Counter stained with Haematoxylin. Secondary antibody only control: Used PBS instead of primary antibody.

    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

    Positive staining on (A) human breast cancer; weak staining on (B) human endometrial cancer; no staining on (C) human colon cancer. The section was incubated with ab209664 for 30 mins at room temperature.
    The immunostaining was performed on a Leica Biosystems BOND® RX instrument.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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