Anti-TRPS1 antibody [EPR16171] - BSA and Azide free

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRPS1 antibody [EPR16171] - BSA and Azide free (AB251509)

This data was developed using ab209664, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded human breast cancer (stage II, left ; stage III right) tissue labeling TRPS1 with ab209664 at 1/8000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

(Left) : Nucleus staining in the cancer cells of breast cancer stage II clinical case; (Right) : Negative staining in the breast cancer stage III clinical case; The high expression of TRPS1 may facilitate improved survival of this breast cancer stage II case.

Counter stained with Hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRPS1 antibody [EPR16171] - BSA and Azide free (AB251509)

This data was developed using ab209664, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded human breast cancer tissue labeling TRPS1 with ab209664 at 1/8000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Nuclear staining on human breast cancer is observed. Counter stained with Hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRPS1 antibody [EPR16171] - BSA and Azide free (AB251509)

This data was developed using ab209664, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded human kidney tissue labeling TRPS1 with ab209664 at 1/8000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Negative staining on the human kidney. Counter stained with Hematoxylin.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRPS1 antibody [EPR16171] - BSA and Azide free (AB251509)

This data was developed using ab209664, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded human breast tissue labeling TRPS1 with ab209664 at 1/8000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Nuclear staining on epithelium of human breast is observed. Counter stained with Hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRPS1 antibody [EPR16171] - BSA and Azide free (AB251509)

This data was developed using ab209664, the same antibody clone in a different buffer formulation. Immunohistochemical analysis of Paraffin-embedded sections human breast (A), endometrium (B) and colon (C) tissue labelling TRPS1 with ab209664 at 1/18000 dilution, followed by a ready to use secondary Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Counter stained with Haematoxylin. Secondary antibody only control : Used PBS instead of primary antibody. Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins. Positive staining on (A) human breast; weak staining on (B) human endometrium; no staining on (C) human colon. The section was incubated with ab209664 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRPS1 antibody [EPR16171] - BSA and Azide free (AB251509)

This data was developed using ab209664, the same antibody clone in a different buffer formulation. Immunohistochemical analysis of Paraffin-embedded sections human breast cancer (A), endometrium cancer(B) and colon caner (C) tissue labelling TRPS1 with ab209664 at 1/18000 dilution, followed by a ready to use secondary Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Counter stained with Haematoxylin. Secondary antibody only control : Used PBS instead of primary antibody. Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins. Positive staining on (A) human breast cancer; weak staining on (B) human endometrial cancer; no staining on (C) human colon cancer. The section was incubated with ab209664 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument.