AB3487

Anti-TRPV1 antibody

Name: Anti-TRPV1 antibody (ab3487) | Abcam
Brand: Abcam Limited
SKU: AB3487
Price: 505 USD
Availability: InStock
Rating: 5 (2 reviews)

(2 Reviews)

(39 Publications)

Anti-TRPV1 antibody (ab3487) is a rabbit polyclonal antibody detecting TRPV1 in IHC-P, IHC-Fr. Suitable for Human.

- Over 30 publications
- Trusted since 2003

View Alternative Names

VR1, TRPV1, Transient receptor potential cation channel subfamily V member 1, TrpV1, Capsaicin receptor, Osm-9-like TRP channel 1, Vanilloid receptor 1, OTRPC1

2 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRPV1 antibody (AB3487)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRPV1 antibody (AB3487)

Immunolocalization of TRPV1 in human glabrous skin using ab3487.

Immunohistochemistry (Frozen sections) - Anti-TRPV1 antibody (AB3487)

IHC-Fr

Supplier Data

Immunohistochemistry (Frozen sections) - Anti-TRPV1 antibody (AB3487)

Immunohistochemistry (frozen sections) analysis of human skin tissue sections labeling TRPV1 (red) with ab3487 at 1/250. A biotin-conjugated donkey anti-rabbit IgG was used as the secondary antibody.

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

IHC-Fr, IHC-P

applications

Immunogen

Synthetic Peptide within Human TRPV1 aa 1-50. The exact immunogen used to generate this antibody is proprietary information.

Q8NER1

Specificity

Immunohistochemical staining of TRPV1 in human glabrous skin using this antibody results in the staining of epidermal and dermal cells, as well as free nerve fibers and Merkel cells. Immunofluorescent staining of TRPV1 in human prostatic urethra results in fibrous staining within the epithelial cells.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "IHCFr" : {"fullname" : "Immunohistochemistry (Frozen sections)", "shortname":"IHC-Fr"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1 µg/mL", "IHCP-species-notes": "<p></p>", "IHCFr-species-checked": "testedAndGuaranteed", "IHCFr-species-dilution-info": "1 µg/mL", "IHCFr-species-notes": "<p></p>" } } }

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Product details

What is this antibody validated in?
Anti-TRPV1 antibody (ab3487) is a rabbit polyclonal antibody and is validated for use in Immunohistochemistry (IHC-P), Immunohistochemistry (IHC-Fr) in Human samples.

Trusted by the scientific community
Anti-TRPV1 (ab3487) was first used in a scientific publication in 2003 and has been cited over 30 times in peer-reviewed journals.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

Preservative: 0.05% Sodium azide Constituents: PBS, 0.1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

TRPV1 also known as the transient receptor potential vanilloid 1 or capsaicin receptor is a non-selective cation channel with a molecular mass of approximately 95 kDa. It acts mechanically by allowing the influx of calcium and sodium ions upon activation. This receptor is expressed predominantly in sensory neurons specifically in dorsal root ganglia and trigeminal ganglia which are key regions involved in pain perception. TRPV1 can also be found in various non-neuronal tissues influencing a range of physiological responses.

Biological function summary

The role of TRPV1 extends beyond sensory perception. It is involved in detecting and regulating body temperature and is activated by heat acidic conditions and certain lipid metabolites. The receptor operates as part of a homotetrameric complex contributing to neural signaling. It is activated by chemical ligands such as capsaicin the compound responsible for the spicy sensation in chili peppers influencing pain and inflammation pathways.

Pathways

TRPV1 plays essential roles in pain and nociception pathways. It intersects with the inflammatory pathway where its activity is modulated by protein kinase C (PKC) and phospholipase C (PLC). These pathways involve calcium signaling and relate to proteins such as PKA and calcineurin which modulate TRPV1 activity through phosphorylation and dephosphorylation affecting the body's response to noxious stimuli.

TRPV1 is a critical player in pain management and inflammatory disorders. It has been linked to chronic pain conditions like neuropathic pain and is also involved in migraine pathophysiology. In these contexts TRPV1 interacts with proteins like CGRP and TRPA1 which are also implicated in pain signaling and migraine mechanisms. Antagonists such as capsazepine AMG 9810 and AMG-517 have been researched for therapeutic targeting of TRPV1 in these disorders.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Ligand-activated non-selective calcium permeant cation channel involved in detection of noxious chemical and thermal stimuli. Seems to mediate proton influx and may be involved in intracellular acidosis in nociceptive neurons. Involved in mediation of inflammatory pain and hyperalgesia. Sensitized by a phosphatidylinositol second messenger system activated by receptor tyrosine kinases, which involves PKC isozymes and PCL. Activation by vanilloids, like capsaicin, and temperatures higher than 42 degrees Celsius, exhibits a time- and Ca(2+)-dependent outward rectification, followed by a long-lasting refractory state. Mild extracellular acidic pH (6.5) potentiates channel activation by noxious heat and vanilloids, whereas acidic conditions (pH <6) directly activate the channel. Can be activated by endogenous compounds, including 12-hydroperoxytetraenoic acid and bradykinin. Acts as ionotropic endocannabinoid receptor with central neuromodulatory effects. Triggers a form of long-term depression (TRPV1-LTD) mediated by the endocannabinoid anandamine in the hippocampus and nucleus accumbens by affecting AMPA receptors endocytosis.

See full target information TRPV1

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Publications (39)

Recent publications for all applications. Explore the full list and refine your search

Scientific reports 14:22809 PubMed39354036

2024

Saxitoxin potentiates human neuronal cell death induced by Zika virus while sparing neural progenitors and astrocytes.

Applications

Unspecified application

Species

Unspecified reactive species

Leticia R Q Souza,Carolina G da S Pedrosa,Teresa Puig-Pijuan,Camila da Silva Dos Santos,Gabriela Vitória,João M A Delou,Pedro Setti-Perdigão,Luiza M Higa,Amilcar Tanuri,Stevens Kastrup Rehen,Marília Zaluar P Guimarães

British journal of pharmacology 181:2676-2696 PubMed38627101

2024

Calcium-sensing receptor regulates Kv7 channels via G protein signalling and modulates excitability of human induced pluripotent stem cell-derived nociceptive-like neurons.

Applications

Unspecified application

Species

Unspecified reactive species

Nontawat Chuinsiri,Nannapat Siraboriphantakul,Luke Kendall,Polina Yarova,Christopher J Nile,Bing Song,Ilona Obara,Justin Durham,Vsevolod Telezhkin

Turkish neurosurgery 34:299-307 PubMed38497183

2024

Low-Level miR-199 Contribute to Neuropathic Low Back Pain via TRPV1 by Regulating the Production of Pro-Inflammatory Cytokines on Macrophage.

Applications

Unspecified application

Species

Unspecified reactive species

Zi Li,Yonghong Li,Zuohua Li

BMC immunology 24:55 PubMed38129779

2023

TRPV1 neurons alter Staphylococcus aureus skin infection outcomes by affecting macrophage polarization and neutrophil recruitment.

Applications

Unspecified application

Species

Unspecified reactive species

Changyu Huang,Yang Chen,Yuanqing Cai,Haiqi Ding,Jiaoying Hong,Shan You,Yiming Lin,Hongxin Hu,Yongfa Chen,Xueni Hu,Yanshu Chen,Ying Huang,Chaofan Zhang,Yunzhi Lin,Zida Huang,Wenbo Li,Wenming Zhang,Xinyu Fang

International journal of molecular sciences 24: PubMed37240443

2023

"ThermoTRP" Channel Expression in Cancers: Implications for Diagnosis and Prognosis (Practical Approach by a Pathologist).

Applications

Unspecified application

Species

Unspecified reactive species

Arpad Szallasi

Acta dermato-venereologica 103:adv4463 PubMed36967545

2023

Neuroimmune Mediators of Pruritus in Hispanic Scalp Psoriatic Itch.

Applications

Unspecified application

Species

Unspecified reactive species

Leigh A Nattkemper,Zoe M Lipman,Giuseppe Ingrasci,Claudia Maldonado,Juan Carlos Garces,Enrique Loayza,Gil Yosipovitch

Journal of the European Academy of Dermatology and Venereology : JEADV 37:e818-e820 PubMed36785978

2023

COVID-19-associated chilblain pruritus is non-histaminergic mediated and correlated to epidermal interleukin 31.

Applications

Unspecified application

Species

Unspecified reactive species

Angelina Labib,Leigh Nattkemper,Ashley Vander Does,Teresa Ju,Sara Cacciapuoti,Maria Vastarella,Gabriella Fabbrocini,Gil Yosipovitch

International journal of molecular sciences 24: PubMed36768564

2023

Gestational Diabetes Mellitus and Small-for-Gestational-Age: An Insight into the Placental Molecular Biomarkers.

Applications

Unspecified application

Species

Unspecified reactive species

Christian Giommi,Marta Lombό,Nina Montik,Michela Paolucci,Valentina Notarstefano,Giovanni Delli Carpini,Andrea Ciavattini,Antonio Ragusa,Francesca Maradonna,Elisabetta Giorgini,Oliana Carnevali

eNeuro 9: PubMed36635242

2022

Localization of TRP Channels in Healthy Oral Mucosa from Human Donors.

Applications

Unspecified application

Species

Unspecified reactive species

Yalda Moayedi,Stephanie Michlig,Mark Park,Alia Koch,Ellen A Lumpkin

Biomolecules 12: PubMed36551194

2022

The Distribution of Innervation and Immune Cell Infiltration Is Different in Genital and Extragenital Variants of Lichen Sclerosus.

Applications

Unspecified application

Species

Unspecified reactive species

Dubravka Vuković,Marin Ogorevc,Ivo Tripković,Neira Puizina-Ivić,Mirna Saraga-Babić,Snježana Mardešić

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-TRPV1 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRPV1 antibody (AB3487)

Immunohistochemistry (Frozen sections) - Anti-TRPV1 antibody (AB3487)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Specificity

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (39)

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