Anti-TRPV1 antibody [BS397] - C-terminal

• WB

Supplier Data

Western blot - Anti-TRPV1 antibody [BS397] - C-terminal (AB203103)

(4°C overnight).

All lanes:

Western blot - Anti-TRPV1 antibody [BS397] - C-terminal (ab203103) at 1 µg/mL

Lane 1:

50B11 treated with Forskolin + NGF at 10 µg

Lane 2:

50B11 treated with Forskolin at 10 µg

Lane 3:

Untreated 50B11 control at 10 µg

Lane 4:

NGF-stimulated PC-12 (Rat glial tumor cell line) cell lysate (NGF control) at 10 µg

Predicted band size: 95 kDa

Observed band size: 95-100 kDa

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• ICC/IF

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Immunocytochemistry/ Immunofluorescence - Anti-TRPV1 antibody [BS397] - C-terminal (AB203103)

ab203103 staining TRPV1 in C6 (Rat glial tumor cell line) cells by ICC/IF (Immunocytochemistry/immunofluorescence).

Cells were fixed with 4% formaldehyde, permeabilized with 0.1% Triton X-100 and blocked with 10% horse serum. Samples were incubated with primary antibody (2 μg/ml, panel B) for 1 hour. A CF488A-conjugated Donkey anti-mouse polyclonal was used as the secondary antibody (5 μg/ml). Nuclei were stained with Hoechst dye (panel A) and panel C shows the merged image.

• IHC-Fr

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Immunohistochemistry (Frozen sections) - Anti-TRPV1 antibody [BS397] - C-terminal (AB203103)

Immunohistochemical analysis of mouse dorsal root ganglia labelling TRPV1 with ab203103 at 10 μg/mL. Visualized with anti-mouse-Cy3 conjugate (red).

• Flow Cyt (Intra)

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Flow Cytometry (Intracellular) - Anti-TRPV1 antibody [BS397] - C-terminal (AB203103)

Analysis of TRPV1 expression in PC-12 (Rat adrenal gland pheochromocytoma cell line) cell line by Flow Cyt (Intra) (black solid).

Cells were fixed and permeabilized in absolute methanol (10 minutes in ice) and 0.1% Tween-20 in PBS, then blocked with 1% BSA. Incubated with primary antibody for 30 minutes at RT. Secondary antibody used is a Goat anti-mouse PE labeled secondary antibody (1 : 100 dilution), 20 minutes in dark at room temperature.

Negative control : Non-specific Control IgG, clone X63 (black dashed).

• WB

Supplier Data

Western blot - Anti-TRPV1 antibody [BS397] - C-terminal (AB203103)

SDS-PAGE : denatured and reduced. ab203103 detects bands at 90-100 kDa and 180-200 kDa corresponding to TRPV1 monomer and dimer.

Lanes 1 and 3:

Western blot - Anti-TRPV1 antibody [BS397] - C-terminal (ab203103) at 2 µg/mL

Lane 2:

Western blot - Anti-TRPV1 antibody [BS397] - C-terminal (ab203103) at 1 µg/mL

All lanes:

Mouse brain homogenate at 25 µg

Secondary

All lanes:

Anti-mouse-HRP at 1/6000 dilution

Predicted band size: 94 kDa

true

• WB

Supplier Data

Western blot - Anti-TRPV1 antibody [BS397] - C-terminal (AB203103)

SDS-PAGE : denatured and reduced

All lanes:

Western blot - Anti-TRPV1 antibody [BS397] - C-terminal (ab203103) at 2 µg/mL

All lanes:

Rat PC-12 (Rat adrenal gland pheochromocytoma cell line) cell lysate at 80 µg

Secondary

All lanes:

Anti-mouse-HRP at 1/6000 dilution

Predicted band size: 94 kDa

true