Rabbit Polyclonal TRPV2 antibody. Suitable for WB, IHC-P and reacts with Mouse, Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human Transient receptor potential cation channel subfamily V member 2.
IgG
Rabbit
pH: 7
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 10% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine
Liquid
Polyclonal
WB | IHC-P | |
---|---|---|
Human | Tested | Tested |
Mouse | Tested | Expected |
Species | Dilution info | Notes |
---|---|---|
Species Mouse | Dilution info 1/500.00000 - 1/3000.00000 | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Species Human | Dilution info 1/500.00000 - 1/3000.00000 | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info 1/100.00000 - 1/1000.00000 | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
Calcium-permeable, non-selective cation channel with an outward rectification. Seems to be regulated, at least in part, by IGF1, PDGF and neuropeptide head activator. May transduce physical stimuli in mast cells. Activated by temperatures higher than 52 degrees Celsius; is not activated by vanilloids and acidic pH.
VRL, TRPV2, VRL, Transient receptor potential cation channel subfamily V member 2, TrpV2, Osm-9-like TRP channel 2, Vanilloid receptor-like protein 1, OTRPC2, VRL-1
Rabbit Polyclonal TRPV2 antibody. Suitable for WB, IHC-P and reacts with Mouse, Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human Transient receptor potential cation channel subfamily V member 2.
IgG
Rabbit
pH: 7
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 10% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine
Liquid
Polyclonal
Affinity purification Immunogen
Blue Ice
1-2 weeks
+4°C
-20°C
Upon delivery aliquot
Avoid freeze / thaw cycle
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This supplementary information is collated from multiple sources and compiled automatically.
TRPV2 also known as transient receptor potential cation channel subfamily V member 2 is a non-selective cation channel with a molecular mass of around 85 kDa. Known for being heat-activated TRPV2 is sensitive to temperatures above 52°C. The protein is expressed in various tissues including heart lung spleen and brain. In neuronal and non-neuronal cells TRPV2 plays a role in calcium influx responding to potential mechanical stretch stimuli and cannabinoids like cannabidiol.
TRPV2 acts as a sensor within cells and is vital for regulating calcium homeostasis affecting cellular responses in many types of cells. TRPV2 is not part of any multi-subunit complex but is often found located on the cell membrane where it modulates membrane potential and intracellular signaling. This protein contributes to tracking endogenous lipids for cellular signaling pathways necessary for various biological processes like muscle contraction and immune response.
TRPV2 engages in cellular signaling pathways that modulate physiological responses. In particular it is part of the mechanotransduction and thermal sensing pathways where it interacts with proteins such as TRPV1 TRPV3 and TRPV4. These pathways are important for interpreting and reacting to mechanical and thermal stimuli influencing processes like inflammation and pain perception.
TRPV2 modulation impacts conditions such as cardiac hypertrophy and neurodegenerative disorders. TRPV2 interacts with proteins like calmodulin and various ion channels affecting cellular calcium dynamics that can lead to pathological states. Studies indicate that TRPV2 may play a part in the pathogenesis of heart diseases providing a link between mechanical stimuli and the development of cardiac-related conditions. Additionally aberrant regulation of TRPV2 has been implicated in muscular dystrophy highlighting its role in muscular maintenance and degradation processes.
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This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
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7.5% SDS PAGE
All lanes: Western blot - Anti-TRPV2 antibody (ab272862) at 1/500 dilution
Lane 1: A431 (human epidermoid carcinoma cell line) whole cell lysate at 30 µg
Lane 2: Raji (human Burkitt's lymphoma cell line) whole cell lysate at 30 µg
All lanes: HRP-conjugated anti-rabbit IgG antibody
Predicted band size: 85 kDa
Paraffin-embedded human lung tissue stained for TRPV2 using ab272862 at 10 μg/mL in immunohistochemical analysis. Antigen retrieval using EDTA based, pH 8.0 buffer, 15 minutes.
7.5% SDS PAGE
All lanes: Western blot - Anti-TRPV2 antibody (ab272862) at 1/1000 dilution
All lanes: NIH/3T3 (mouse embryo fibroblast cell line) whole cell lysate at 30 µg
All lanes: HRP-conjugated anti-rabbit IgG antibody
Predicted band size: 85 kDa
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