AB94582

Anti-TRPV3 antibody [N15/39]

Name: Anti-TRPV3 antibody [N15/39] (ab94582) | Abcam
Brand: Abcam Limited
SKU: AB94582
Price: 525 USD
Availability: InStock

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(1 Publication)

Mouse Monoclonal TRPV3 antibody. Suitable for Flow Cyt, IHC-P, ICC/IF and reacts with Human, Mouse samples. Cited in 1 publication.

3 Images

Immunocytochemistry/ Immunofluorescence - Anti-TRPV3 antibody [N15/39] (AB94582)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-TRPV3 antibody [N15/39] (AB94582)

ice cold methanol-fixed HaCat cells labeling TRPV3 (green) using ab94582 at 1/100 dilution in ICC/IF analysis. FITC Goat Anti-Mouse (green) was used as the secondary antibody at 1/50 dilution. Dotty staining in all cells. Some intermediate filamentlike staining in some cells was observed.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRPV3 antibody [N15/39] (AB94582)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRPV3 antibody [N15/39] (AB94582)

Paraffin-embedded, Bouin's-fixed mouse backskin tissue stained for TRPV3 (green) using ab94582 at 1/100 dilution in immunohistochemical analysis. FITC Goat Anti-Mouse (green) was used as the secondary antibody at 1/50 dilution. Filaggrinlike staining in upper layers. Dull lower layer cell staining. Some stain seen in hypodermis is observed.

Flow Cytometry - Anti-TRPV3 antibody [N15/39] (AB94582)

Flow Cyt

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Flow Cytometry - Anti-TRPV3 antibody [N15/39] (AB94582)

Overlay histogram showing SH-SY5Y cells stained with ab94582 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab94582, 1μg/1x10⁶ cells) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-mouse IgG (H+L) (ab150113) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG2a [ICIGG2A] (ab91361, 1μg/1x10⁶ cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive result in 80% methanol (5 min) fixed SH-SY5Y cells used under the same conditions. Please note that Abcam do not have any data for use of this antibody on non-fixed cells. We welcome any customer feedback.

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

N15/39

Isotype

IgG2a

Carrier free

Reacts with

Mouse, Human

Applications

ICC/IF, IHC-P, Flow Cyt

applications

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "0.1-1 µg for 10^6 Cells", "FlowCyt-species-notes": "<a href='/en-us/products/primary-antibodies/mouse-igg2c-kappa-monoclonal-18c8bc7ad10-isotype-control-ab170191'>ab170191</a> - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1-10 µg/mL", "ICCIF-species-notes": "" }, "Mouse": { "FlowCyt-species-checked": "guaranteed", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "0.1-1 µg/mL", "IHCP-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Product details

The clone number has been updated from S15-39 to N15/39, both clone numbers name the same antibody clone.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein G

Storage buffer

Preservative: 0.09% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine)

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The TRPV3 protein also known as transient receptor potential cation channel subfamily V member 3 functions as a calcium-permeable channel primarily involved in thermoregulation. With a mass of approximately 91 kDa TRPV3 is activated by warm temperatures and certain chemical compounds including natural oils. It is widely expressed in keratinocytes of the skin as well as in sensory neurons and can be found in other tissues like the brain and hair follicles.

Biological function summary

TRPV3 contributes to temperature sensation and pain perception playing an important role in maintaining skin barrier homeostasis. TRPV3 functions as a homotetramer forming a channel that allows the entry of cations into cells. This channel activity regulates various processes including cell proliferation differentiation and survival especially in the skin cells. Mutations or dysregulation in TRPV3 can result in abnormal skin physiology highlighting its function in epidermal biology.

Pathways

TRPV3 interacts closely with the TRP channel family and integrates into the calcium signaling and MAPK signaling pathways. Within these pathways TRPV3 modulates intracellular calcium levels affecting processes like inflammation and pain signaling. It closely associates with other TRP channels such as TRPV1 which is involved in mediating pain and heat sensation providing a coordinated response to environmental changes.

TRPV3 is linked to conditions such as Olmsted syndrome and atopic dermatitis both characterized by skin abnormalities. In Olmsted syndrome mutations in TRPV3 disrupt normal calcium influx leading to symptoms like palmoplantar keratoderma. Additionally TRPV3 dysregulation might exacerbate atopic dermatitis possibly through interactions with the protein filaggrin which is important for skin barrier function. Understanding TRPV3's role in these contexts can offer insights into potential therapeutic targets for skin-related disorders.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

See full target information TRPV3

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

International journal of molecular medicine 35:597-606 PubMed25571999

2015

Upregulation of RBFOX1 in the malformed cortex of patients with intractable epilepsy and in cultured rat neurons.

Applications

Unspecified application

Species

Unspecified reactive species

Ming Wen,Yong Yan,Ning Yan,Xiao Shan Chen,Shi Yong Liu,Zhan Hui Feng

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-TRPV3 antibody [N15/39]

Immunocytochemistry/ Immunofluorescence - Anti-TRPV3 antibody [N15/39] (AB94582)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRPV3 antibody [N15/39] (AB94582)

Flow Cytometry - Anti-TRPV3 antibody [N15/39] (AB94582)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

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