Anti-TRPV3 antibody [N15/4]

• Flow Cyt

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Flow Cytometry - Anti-TRPV3 antibody [N15/4] (AB85022)

Overlay histogram showing SH-SY5Y cells stained with ab85022 (red line). The cells were fixed with 80% methanol (5 min) and incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab85022, 1μg/1x10⁶ cells) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-mouse IgG (H&L) (ab150113) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG2a [ICIGG2A] (ab91361, 1μg/1x10⁶ cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. Please note that Abcam do not have any data for use of this antibody on non-fixed cells. We welcome any customer feedback.

• IHC-P

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRPV3 antibody [N15/4] (AB85022)

Immunohistochemical analysis of Bouin's Fixative and paraffin-embedded Human hippocampus tissue labelling TrpV3 with ab85022 at 1/1000 dilution (1 hour at RT), followed by FITC Goat Anti-Mouse (green) at 1/50 (1 hour at RT).

• IHC-P

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRPV3 antibody [N15/4] (AB85022)

Immunohistochemical analysis of Bouin's Fixative and paraffin-embedded Mouse backskin tissue labelling TrpV3 with ab85022 at 1/100 dilution (1 hour at RT), followed by FITC Goat Anti-Mouse (green) at 1/50 (1 hour at RT).

• WB

Supplier Data

Western blot - Anti-TRPV3 antibody [N15/4] (AB85022)

Western blot analysis using ab85022 showing detection of Human TrpV3 protein in a transient over-expression lysate.

Color Development : Chemiluminescent for HRP (Moss) for 30 sec in RT. Exposed 0.5 seconds.

NOTE : The ORF clone contains a C-term DDK tag; results were confirmed using Anti-DDK antibody showing laddering below target is related to variants or degradation products of the TRPV3 protein.

All lanes:

Western blot - Anti-TRPV3 antibody [N15/4] (ab85022) at 1/1000 dilution

Lanes 1 and 3:

HEK293T over-expression at 5 µg

Lanes 2 and 4:

HEK293T negative control at 5 µg

Secondary

All lanes:

Goat anti-mouse IgG:HRP for 1 hour at RT at 1/5000 dilution

Observed band size: 90 kDa

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Exposure time: 2hr