Anti-TRPV4 antibody

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRPV4 antibody (AB191580)

Immunohistochemical analysis of paraffin-embedded human lung tissue labeling TRPV4 with ab191580 at 5 μg/ml.

• ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-TRPV4 antibody (AB191580)

Immunofluorescent analysis of human lung tissue labeling TRPV4 with ab191580 at 20 μg/ml.

• ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-TRPV4 antibody (AB191580)

Immunofluorescent analysis of TRPV4 expression in human kidney tissue with ab191580 at 5µg/ml.

• ICC

AbReview82505****

Immunocytochemistry - Anti-TRPV4 antibody (AB191580)

Immunocytochemistry analysis of formaldehyde-fixed 0.1% triton x100 permeabilized human chondrocyte cells staining with ab191580 at 1/100 dilution. Secondary antibody was Alexa Fluor® 488 Goat anti rabbit antibody at 1/1000 dilution. Samples were incubated with the primary antibody with PBS with 0.1% triton x100 for 30 minutes at 37°C. Blocking was done using 1% BSA for 15 minutes at 37°C.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRPV4 antibody (AB191580)

Immunohistochemical analysis of paraffin-embedded human liver tissue using ab191580 at 2 μg/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4°C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counterstained with Hematoxylin.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRPV4 antibody (AB191580)

Immunohistochemical analysis of paraffin-embedded rat kidney tissue using ab191580 at 2 μg/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4°C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counterstained with Hematoxylin.

• WB

Unknown

Western blot - Anti-TRPV4 antibody (AB191580)

Western Blot of human testis tissue lysate, SK-N-SH cell lysate labeling TRPV4 with Anti-TRPV4 antibody (ab191580) at 1μg/ml.

All lanes:

Western blot - Anti-TRPV4 antibody (ab191580)

Predicted band size: 98 kDa

false

• WB

Supplier Data

Western blot - Anti-TRPV4 antibody (AB191580)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

All lanes:

Western blot - Anti-TRPV4 antibody (ab191580) at 0.5 µg/mL

Lane 1:

Human kidney tissue at 15 µg

Lane 2:

Human lung tissue at 15 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG HRP conjugate at 1/10000 dilution

Predicted band size: 98 kDa

Observed band size: 98 kDa

false

• WB

Supplier Data

Western blot - Anti-TRPV4 antibody (AB191580)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

All lanes:

Western blot - Anti-TRPV4 antibody (ab191580) at 0.5 µg/mL

Lane 1:

HepG2 at 15 µg

Lane 2:

SK-N-SH at 15 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG HRP conjugate at 1/10000 dilution

Predicted band size: 98 kDa

Observed band size: 98 kDa

false

• WB

Supplier Data

Western blot - Anti-TRPV4 antibody (AB191580)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

All lanes:

Western blot - Anti-TRPV4 antibody (ab191580) at 0.5 µg/mL

Lane 1:

Rat kidney tissue at 15 µg

Lane 2:

Rat lung tissue at 15 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG HRP conjugate at 1/10000 dilution

Predicted band size: 98 kDa

Observed band size: 98 kDa

false

• WB

Supplier Data

Western blot - Anti-TRPV4 antibody (AB191580)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

All lanes:

Western blot - Anti-TRPV4 antibody (ab191580) at 0.5 µg/mL

Lane 1:

Mouse kidney tissue at 15 µg

Lane 2:

Mouse lung tissue at 15 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG HRP conjugate at 1/10000 dilution

Predicted band size: 98 kDa

Observed band size: 98 kDa

false

• WB

Supplier Data

Western blot - Anti-TRPV4 antibody (AB191580)

All lanes:

Western blot - Anti-TRPV4 antibody (ab191580) at 1 µg/mL

All lanes:

Human testis tissue lysate at 15 µg

Predicted band size: 98 kDa

false