AB231772

Anti-TRPV4 antibody [EPR22622-54]

20ul selling size
BOND RX™ Validated
RabMAb
Recombinant
What is this?

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(1 Publication)

Rabbit Recombinant Monoclonal TRPV4 antibody. Suitable for IHC-P and reacts with Mouse, Rat samples. Cited in 1 publication.

View Alternative Names

Trp12, Vrl2, Vroac, Trpv4, Transient receptor potential cation channel subfamily V member 4, TrpV4, Osm-9-like TRP channel 4, Transient receptor potential protein 12, Vanilloid receptor-like channel 2, Vanilloid receptor-like protein 2, Vanilloid receptor-related osmotically-activated channel, OTRPC4, TRP12, VR-OAC

4 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRPV4 antibody [EPR22622-54] (AB231772)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRPV4 antibody [EPR22622-54] (AB231772)

Immunohistochemical analysis of paraffin-embedded rat spleen tissue labeling TRPV4 with ab231772 at 1/100 dilution, followed by the ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Membranous staining on blood vessels of rat spleen (PMID : 16627472) is observed. The section was incubated with ab231772 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND^® RX instrument. Counterstained with hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRPV4 antibody [EPR22622-54] (AB231772)

Immunohistochemical analysis of paraffin-embedded mouse kidney tissue labeling TRPV4 with ab231772 at 1/100 dilution, followed by the ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Membranous staining on mouse kidney (PMID : 16627472, 18499744) is observed. The section was incubated with ab231772 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND^® RX instrument. Counterstained with hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRPV4 antibody [EPR22622-54] (AB231772)

Immunohistochemical analysis of paraffin-embedded mouse cerebrum tissue labeling TRPV4 with ab231772 at 1/100 dilution, followed by the ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Membranous staining on choroid plexus of mouse cerebrum (PMID : 18499744) is observed. The section was incubated with ab231772 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND^® RX instrument. Counterstained with hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRPV4 antibody [EPR22622-54] (AB231772)

Immunohistochemical analysis of paraffin-embedded rat kidney tissue labeling TRPV4 with ab231772 at 1/100 dilution, followed by the ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Membranous staining on rat kidney (PMID : 16627472, 18499744) is observed. The section was incubated with ab231772 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND^® RX instrument. Counterstained with hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.

Carrier free

Anti-TRPV4 antibody [EPR22622-54] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR22622-54

Isotype

IgG

Carrier free

Reacts with

Mouse, Rat

Applications

IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IHCFr" : {"fullname" : "Immunohistochemistry (Frozen sections)", "shortname":"IHC-Fr"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Mouse": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCFr-species-checked": "notRecommended", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100", "IHCP-species-notes": "" }, "Rat": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCFr-species-checked": "notRecommended", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100", "IHCP-species-notes": "" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Conditional Ambient

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

TRPV4 also known as transient receptor potential vanilloid 4 is an ion channel protein that plays a mechanical role in the human body. This protein with a mass of approximately 98 kDa is sensitive to osmotic pressure mechanical forces and temperature changes. It acts as a calcium-permeable non-selective cation channel. TRPV4 is widely expressed in various tissues including the choroid plexus as well as in the epithelial cells endothelium and certain neurons. The expression pattern suggests its involvement in various physiological processes.

Biological function summary

TRPV4 serves functions essential for osmoregulation mechanosensation and thermosensation. It often forms a complex with other proteins to modulate calcium influx which influences cellular responses. TRPV4 impacts various biological processes such as maintaining cell volume and adapting to temperature changes. Its role in regulating fluid movement across barriers like those in the choroid plexus demonstrates its involvement in maintaining homeostasis. These interactions highlight the protein's widespread influence on various cellular functions.

Pathways

TRPV4 integrates into critical signal transduction pathways that include the mechanotransduction and osmoregulation pathways. It is closely related to other TRP family members such as TRPV1 and TRPC1 within these pathways. The mechanotransduction pathway allows it to convey mechanical stimuli into electrochemical activity impacting various systems throughout the body. The osmoregulation pathway highlights its ability to sense and respond to osmotic shifts maintaining cellular and systemic equilibrium.

TRPV4 has significant links to a range of pathologies such as skeletal dysplasias and neuropathies. Mutations in TRPV4 can lead to altered channel function contributing to conditions like Charcot-Marie-Tooth disease and congenital distal spinal muscular atrophy. These associations often involve the misregulation of calcium homeostasis where TRPV4's interaction with proteins like TRPV1 leads to a disruption of normal cellular processes highlighting the importance of maintaining TRPV4's proper function to prevent those diseases.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Non-selective calcium permeant cation channel involved in osmotic sensitivity and mechanosensitivity (PubMed : 11094154, PubMed : 12093812, PubMed : 12538589). Activation by exposure to hypotonicity within the physiological range exhibits an outward rectification (PubMed : 12093812, PubMed : 14691263, PubMed : 16368742, PubMed : 16571723). Also activated by heat, low pH, citrate and phorbol esters (PubMed : 14691263). Increase of intracellular Ca(2+) potentiates currents. Channel activity seems to be regulated by a calmodulin-dependent mechanism with a negative feedback mechanism (By similarity). Acts as a regulator of intracellular Ca(2+) in synoviocytes (By similarity). Plays an obligatory role as a molecular component in the nonselective cation channel activation induced by 4-alpha-phorbol 12,13-didecanoate and hypotonic stimulation in synoviocytes and also regulates production of IL-8 (By similarity). Together with PKD2, forms mechano- and thermosensitive channels in cilium (PubMed : 18695040). Promotes cell-cell junction formation in skin keratinocytes and plays an important role in the formation and/or maintenance of functional intercellular barriers (PubMed : 20413591). Negatively regulates expression of PPARGC1A, UCP1, oxidative metabolism and respiration in adipocytes (PubMed : 23021218). Regulates expression of chemokines and cytokines related to pro-inflammatory pathway in adipocytes (PubMed : 23021218). Together with AQP5, controls regulatory volume decrease in salivary epithelial cells (PubMed : 16571723). Required for normal development and maintenance of bone and cartilage (By similarity). In its inactive state, may sequester DDX3X at the plasma membrane. When activated, the interaction between both proteins is affected and DDX3X relocalizes to the nucleus (By similarity). In neurons of the central nervous system, could play a role in triggering voluntary water intake in response to increased sodium concentration in body fluid (PubMed : 27252474).

See full target information Trpv4

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Frontiers in pain research (Lausanne, Switzerland) 6:1532885 PubMed40144515

2025

TRPV4 activation in Schwann cells mediates mechanically induced pain of oral cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Yatendra Mulpuri,Nguyen H Tu,Kenji Inoue,Grace Harden,Samuel J Nicholson,Anisa Seenauth,Yan Huang,Keylin G Escobar,Yalda Moayedi,Nigel W Bunnett,Donna G Albertson,Brian L Schmidt

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-TRPV4 antibody [EPR22622-54]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRPV4 antibody [EPR22622-54] (AB231772)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRPV4 antibody [EPR22622-54] (AB231772)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRPV4 antibody [EPR22622-54] (AB231772)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRPV4 antibody [EPR22622-54] (AB231772)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

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Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

Product promise