Rabbit Recombinant Monoclonal TRPV4 antibody. Suitable for IHC-Fr, IHC-P and reacts with Mouse, Rat samples. Cited in 2 publications.
Images
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRPV4 antibody [EPR23846-44] (AB259361), expandable thumbnail](https://content.abcam.com/products/images/trpv4-antibody-epr23846-44-ab259361--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img172101.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRPV4 antibody [EPR23846-44] (AB259361)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRPV4 antibody [EPR23846-44] (AB259361), expandable thumbnail](https://content.abcam.com/products/images/trpv4-antibody-epr23846-44-ab259361--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img169670.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRPV4 antibody [EPR23846-44] (AB259361)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRPV4 antibody [EPR23846-44] (AB259361), expandable thumbnail](https://content.abcam.com/products/images/trpv4-antibody-epr23846-44-ab259361--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img171220.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRPV4 antibody [EPR23846-44] (AB259361)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRPV4 antibody [EPR23846-44] (AB259361), expandable thumbnail](https://content.abcam.com/products/images/trpv4-antibody-epr23846-44-ab259361--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img178708.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRPV4 antibody [EPR23846-44] (AB259361)")
![Immunohistochemistry (Frozen sections) - Anti-TRPV4 antibody [EPR23846-44] (AB259361), expandable thumbnail](https://content.abcam.com/products/images/trpv4-antibody-epr23846-44-ab259361--immunohistochemistry-frozen-sections-img167325.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Frozen sections) - Anti-TRPV4 antibody [EPR23846-44] (AB259361)")
Publications
Filter by
- Frontiers in cellular neuroscience 17:11497932023Pharmacological activation of transient receptor potential vanilloid 4 promotes triggering of the swallowing reflex in rats.Applications:IHC-Fr, IHCReactive species:Rat, MouseMohammad Zakir Hossain,Hiroshi Ando,Shumpei Unno,Rita Rani Roy,Junichi KitagawaPubMed 36909278
- Frontiers in physiology 13:9270782022Activation of TRPV4 by lactate as a critical mediator of renal fibrosis in spontaneously hypertensive rats after moderate- and high-intensity exercise.Applications:IHC-PReactive species:RatBinyi Zhao,Yanping Xu,Yunlin Chen,Ying Cai,Zhiyan Gong,Dan Li,Hongyu Kuang,Xiaozhu Liu,Hao Zhou,Guochun Liu,Yuehui YinPubMed 36160854
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59.94% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| ICC/IF | IP | Flow Cyt | IHC-Fr | IHC-P | WB | |
|---|---|---|---|---|---|---|
| Human | Not recommended | Not recommended | Not recommended | Not recommended | Not recommended | Not recommended |
| Mouse | Not recommended | Not recommended | Not recommended | Tested | Tested | Not recommended |
| Rat | Not recommended | Not recommended | Not recommended | Tested | Tested | Not recommended |
ICC/IF
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Species Rat | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Species Human | Dilution info - | Notes - |
IP
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Human | Dilution info - | Notes - |
Flow Cyt
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Human | Dilution info - | Notes - |
IHC-Fr
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/100 | Notes - |
Species Rat | Dilution info 1/100 | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/500 | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Species Rat | Dilution info 1/500 | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
WB
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat | Dilution info - | Notes - |
Target data
Function
Non-selective calcium permeant cation channel involved in osmotic sensitivity and mechanosensitivity (PubMed:11081638). Activation by exposure to hypotonicity within the physiological range exhibits an outward rectification (PubMed:11081638). Also activated by heat, low pH, citrate and phorbol esters (By similarity). Increase of intracellular Ca(2+) potentiates currents (By similarity). Channel activity seems to be regulated by a calmodulin-dependent mechanism with a negative feedback mechanism (By similarity). Acts as a regulator of intracellular Ca(2+) in synoviocytes (By similarity). Plays an obligatory role as a molecular component in the nonselective cation channel activation induced by 4-alpha-phorbol 12,13-didecanoate and hypotonic stimulation in synoviocytes and also regulates production of IL-8 (By similarity). Together with PKD2, forms mechano- and thermosensitive channels in cilium (By similarity). Promotes cell-cell junction formation in skin keratinocytes and plays an important role in the formation and/or maintenance of functional intercellular barriers (By similarity). Negatively regulates expression of PPARGC1A, UCP1, oxidative metabolism and respiration in adipocytes (By similarity). Regulates expression of chemokines and cytokines related to pro-inflammatory pathway in adipocytes (By similarity). Together with AQP5, controls regulatory volume decrease in salivary epithelial cells (By similarity). Required for normal development and maintenance of bone and cartilage (By similarity). In its inactive state, may sequester DDX3X at the plasma membrane. When activated, the interaction between both proteins is affected and DDX3X relocalizes to the nucleus (By similarity). In neurons of the central nervous system, could play a role in triggering voluntary water intake in response to increased sodium concentration in body fluid (By similarity).
Alternative names
Vroac, Trpv4, Transient receptor potential cation channel subfamily V member 4, TrpV4, Osm-9-like TRP channel 4, Vanilloid receptor-related osmotically-activated channel, OTRPC4, VR-OAC
Recommended products
Rabbit Recombinant Monoclonal TRPV4 antibody. Suitable for IHC-Fr, IHC-P and reacts with Mouse, Rat samples. Cited in 2 publications.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59.94% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Clone number
- EPR23846-44
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
TRPV4 also known as transient receptor potential vanilloid 4 is an ion channel protein that plays a mechanical role in the human body. This protein with a mass of approximately 98 kDa is sensitive to osmotic pressure mechanical forces and temperature changes. It acts as a calcium-permeable non-selective cation channel. TRPV4 is widely expressed in various tissues including the choroid plexus as well as in the epithelial cells endothelium and certain neurons. The expression pattern suggests its involvement in various physiological processes.
- Biological function summary
TRPV4 serves functions essential for osmoregulation mechanosensation and thermosensation. It often forms a complex with other proteins to modulate calcium influx which influences cellular responses. TRPV4 impacts various biological processes such as maintaining cell volume and adapting to temperature changes. Its role in regulating fluid movement across barriers like those in the choroid plexus demonstrates its involvement in maintaining homeostasis. These interactions highlight the protein's widespread influence on various cellular functions.
- Pathways
TRPV4 integrates into critical signal transduction pathways that include the mechanotransduction and osmoregulation pathways. It is closely related to other TRP family members such as TRPV1 and TRPC1 within these pathways. The mechanotransduction pathway allows it to convey mechanical stimuli into electrochemical activity impacting various systems throughout the body. The osmoregulation pathway highlights its ability to sense and respond to osmotic shifts maintaining cellular and systemic equilibrium.
- Associated diseases and disorders
TRPV4 has significant links to a range of pathologies such as skeletal dysplasias and neuropathies. Mutations in TRPV4 can lead to altered channel function contributing to conditions like Charcot-Marie-Tooth disease and congenital distal spinal muscular atrophy. These associations often involve the misregulation of calcium homeostasis where TRPV4's interaction with proteins like TRPV1 leads to a disruption of normal cellular processes highlighting the importance of maintaining TRPV4's proper function to prevent those diseases.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
8 product images
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRPV4 antibody [EPR23846-44] (ab259361), expandable thumbnail](https://content.abcam.com/products/images/trpv4-antibody-epr23846-44-ab259361--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img172101.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRPV4 antibody [EPR23846-44] (ab259361)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRPV4 antibody [EPR23846-44] (ab259361)
Immunohistochemical analysis of paraffin-embedded Rat cerebrum tissue labeling TRPV4 with ab259361 at 1/500 (1.008 ug/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101). Positive staining in choroid plexus of rat cerebrum (PMID:18499744). The section was incubated with ab259361 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRPV4 antibody [EPR23846-44] (ab259361), expandable thumbnail](https://content.abcam.com/products/images/trpv4-antibody-epr23846-44-ab259361--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img169670.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRPV4 antibody [EPR23846-44] (ab259361)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRPV4 antibody [EPR23846-44] (ab259361)
Immunohistochemical analysis of paraffin-embedded Rat kidney tissue labeling TRPV4 with ab259361 at 1/500 (1.008 ug/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101). Positive staining in rat kidney (PMID:18499744). The section was incubated with ab259361 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRPV4 antibody [EPR23846-44] (ab259361), expandable thumbnail](https://content.abcam.com/products/images/trpv4-antibody-epr23846-44-ab259361--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img171220.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRPV4 antibody [EPR23846-44] (ab259361)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRPV4 antibody [EPR23846-44] (ab259361)
Immunohistochemical analysis of paraffin-embedded Mouse cerebrum tissue labeling TRPV4 with ab259361 at 1/500 (1.008 ug/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101). Positive staining in choroid plexus of mouse cerebrum. The section was incubated with ab259361 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRPV4 antibody [EPR23846-44] (ab259361), expandable thumbnail](https://content.abcam.com/products/images/trpv4-antibody-epr23846-44-ab259361--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img178708.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRPV4 antibody [EPR23846-44] (ab259361)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRPV4 antibody [EPR23846-44] (ab259361)
Immunohistochemical analysis of paraffin-embedded Mouse kidney tissue labeling TRPV4 with ab259361 at 1/500 (1.008 ug/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101). Positive staining in mouse kidney. The section was incubated with ab259361 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins
![Immunohistochemistry (Frozen sections) - Anti-TRPV4 antibody [EPR23846-44] (ab259361), expandable thumbnail](https://content.abcam.com/products/images/trpv4-antibody-epr23846-44-ab259361--immunohistochemistry-frozen-sections-img167325.jpg "Immunohistochemistry (Frozen sections) - Anti-TRPV4 antibody [EPR23846-44] (ab259361)")
Immunohistochemistry (Frozen sections) - Anti-TRPV4 antibody [EPR23846-44] (ab259361)
Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Mouse cerebrum(fresh) tissue labeling TRPV4 with ab259361 at 1/100 (5.04 ug/ml) dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 dilution (Green). Cytoplasmic staining on choroid plexus of mouse cerebrum is observed. The nuclear counterstain was DAPI (Blue).
Secondary antibody control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbedat 1/1000 dilution.
![Immunohistochemistry (Frozen sections) - Anti-TRPV4 antibody [EPR23846-44] (ab259361), expandable thumbnail](https://content.abcam.com/products/images/trpv4-antibody-epr23846-44-ab259361--immunohistochemistry-frozen-sections-img179956.jpg "Immunohistochemistry (Frozen sections) - Anti-TRPV4 antibody [EPR23846-44] (ab259361)")
Immunohistochemistry (Frozen sections) - Anti-TRPV4 antibody [EPR23846-44] (ab259361)
Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Rat cerebrum (fresh) tissue labeling TRPV4 with ab259361 at 1/100 (5.04 ug/ml) dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 dilution (Green). Cytoplasmic staining on choroid plexus of rat cerebrum is observed. The nuclear counterstain was DAPI (Blue).
Secondary antibody control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbedat 1/1000 dilution.
![Immunohistochemistry (Frozen sections) - Anti-TRPV4 antibody [EPR23846-44] (ab259361), expandable thumbnail](https://content.abcam.com/products/images/trpv4-antibody-epr23846-44-ab259361--immunohistochemistry-frozen-sections-img164780.jpg "Immunohistochemistry (Frozen sections) - Anti-TRPV4 antibody [EPR23846-44] (ab259361)")
Immunohistochemistry (Frozen sections) - Anti-TRPV4 antibody [EPR23846-44] (ab259361)
Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Mouse kidney (fresh) tissue labeling TRPV4 with ab259361 at 1/100 (5.04 ug/ml) dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 dilution (Green). Cytoplasmic staining on mouse kidney is observed. The nuclear counterstain was DAPI (Blue).
Secondary antibody control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbedat 1/1000 dilution.
![Immunohistochemistry (Frozen sections) - Anti-TRPV4 antibody [EPR23846-44] (ab259361), expandable thumbnail](https://content.abcam.com/products/images/trpv4-antibody-epr23846-44-ab259361--immunohistochemistry-frozen-sections-img167851.jpg "Immunohistochemistry (Frozen sections) - Anti-TRPV4 antibody [EPR23846-44] (ab259361)")
Immunohistochemistry (Frozen sections) - Anti-TRPV4 antibody [EPR23846-44] (ab259361)
Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Rat kidney (fresh) tissue labeling TRPV4 with ab259361 at 1/100 (5.04 ug/ml) dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 dilution (Green). Cytoplasmic staining on rat kidney is observed. The nuclear counterstain was DAPI (Blue).
Secondary antibody control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbedat 1/1000 dilution.
![Immunohistochemistry (Frozen sections) - Anti-TRPV4 antibody [EPR23846-44] (ab259361), expandable thumbnail](https://content.abcam.com/products/images/trpv4-antibody-epr23846-44-ab259361--immunohistochemistry-frozen-sections-img179956.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Frozen sections) - Anti-TRPV4 antibody [EPR23846-44] (ab259361)")
![Immunohistochemistry (Frozen sections) - Anti-TRPV4 antibody [EPR23846-44] (ab259361), expandable thumbnail](https://content.abcam.com/products/images/trpv4-antibody-epr23846-44-ab259361--immunohistochemistry-frozen-sections-img164780.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Frozen sections) - Anti-TRPV4 antibody [EPR23846-44] (ab259361)")
![Immunohistochemistry (Frozen sections) - Anti-TRPV4 antibody [EPR23846-44] (ab259361), expandable thumbnail](https://content.abcam.com/products/images/trpv4-antibody-epr23846-44-ab259361--immunohistochemistry-frozen-sections-img167851.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Frozen sections) - Anti-TRPV4 antibody [EPR23846-44] (ab259361)")
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