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Rabbit Recombinant Monoclonal TRPV4 antibody. Suitable for WB, IP, IHC-Fr, IHC-P and reacts with Mouse, Human samples. Cited in 1 publication.

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Images

Western blot - Anti-TRPV4 antibody [EPR27068-39] (AB307444), expandable thumbnail
  • Immunoprecipitation - Anti-TRPV4 antibody [EPR27068-39] (AB307444), expandable thumbnail
  • Western blot - Anti-TRPV4 antibody [EPR27068-39] (AB307444), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRPV4 antibody [EPR27068-39] (AB307444), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRPV4 antibody [EPR27068-39] (AB307444), expandable thumbnail

Publications

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form

Liquid

Clonality

Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
WBIPFlow Cyt (Intra)ICC/IFIHC-FrIHC-P
Human
Tested
Tested
Not recommended
Not recommended
Expected
Tested
Mouse
Tested
Not recommended
Not recommended
Not recommended
Tested
Tested
Rat
Not recommended
Not recommended
Not recommended
Not recommended
Not recommended
Not recommended

Tested
Tested

Species

Mouse

Dilution info

1/1000

Notes

-

Species

Human

Dilution info

1/1000

Notes

-

Not recommended
Not recommended

Species

Rat

Dilution info

-

Notes

-

Tested
Tested

Species

Human

Dilution info

1/30

Notes

-

Not recommended
Not recommended

Species

Rat, Mouse

Dilution info

-

Notes

-

Not recommended
Not recommended

Species

Human, Mouse, Rat

Dilution info

-

Notes

-

Not recommended
Not recommended

Species

Human, Mouse, Rat

Dilution info

-

Notes

-

Tested
Tested

Species

Mouse

Dilution info

1/100

Notes

-

Expected
Expected

Species

Human

Dilution info

Use at an assay dependent concentration.

Notes

-

Not recommended
Not recommended

Species

Rat

Dilution info

-

Notes

-

Tested
Tested

Species

Mouse

Dilution info

1/50 - 1/1000

Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Species

Human

Dilution info

1/50 - 1/1000

Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Not recommended
Not recommended

Species

Rat

Dilution info

-

Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Target data

Function

Non-selective calcium permeant cation channel involved in osmotic sensitivity and mechanosensitivity (PubMed:16293632, PubMed:18695040, PubMed:18826956, PubMed:22526352, PubMed:23136043, PubMed:29899501). Activation by exposure to hypotonicity within the physiological range exhibits an outward rectification (PubMed:18695040, PubMed:18826956, PubMed:29899501). Also activated by heat, low pH, citrate and phorbol esters (PubMed:16293632, PubMed:18695040, PubMed:18826956, PubMed:20037586, PubMed:21964574, PubMed:25256292). Increase of intracellular Ca(2+) potentiates currents. Channel activity seems to be regulated by a calmodulin-dependent mechanism with a negative feedback mechanism (PubMed:12724311, PubMed:18826956). Promotes cell-cell junction formation in skin keratinocytes and plays an important role in the formation and/or maintenance of functional intercellular barriers (By similarity). Acts as a regulator of intracellular Ca(2+) in synoviocytes (PubMed:19759329). Plays an obligatory role as a molecular component in the nonselective cation channel activation induced by 4-alpha-phorbol 12,13-didecanoate and hypotonic stimulation in synoviocytes and also regulates production of IL-8 (PubMed:19759329). Together with PKD2, forms mechano- and thermosensitive channels in cilium (PubMed:18695040). Negatively regulates expression of PPARGC1A, UCP1, oxidative metabolism and respiration in adipocytes (By similarity). Regulates expression of chemokines and cytokines related to pro-inflammatory pathway in adipocytes (By similarity). Together with AQP5, controls regulatory volume decrease in salivary epithelial cells (By similarity). Required for normal development and maintenance of bone and cartilage (PubMed:26249260). In its inactive state, may sequester DDX3X at the plasma membrane. When activated, the interaction between both proteins is affected and DDX3X relocalizes to the nucleus (PubMed:29899501). In neurons of the central nervous system, could play a role in triggering voluntary water intake in response to increased sodium concentration in body fluid (By similarity).Isoform 1Non-selective calcium permeant cation channel involved in osmotic sensitivity and mechanosensitivity. Activation by exposure to hypotonicity within the physiological range exhibits an outward rectification. Also activated by phorbol esters. Has the same channel activity as isoform 1, and is activated by the same stimuli.Isoform 5Non-selective calcium permeant cation channel involved in osmotic sensitivity and mechanosensitivity. Activation by exposure to hypotonicity within the physiological range exhibits an outward rectification. Also activated by phorbol esters. Has the same channel activity as isoform 1, and is activated by the same stimuli.Isoform 2Lacks channel activity, due to impaired oligomerization and intracellular retention.Isoform 4Lacks channel activity, due to impaired oligomerization and intracellular retention.Isoform 6Lacks channel activity, due to impaired oligomerization and intracellular retention.(Microbial infection) Facilitates hepatitis C virus (HCV) replication, possibly through its action on DDX3X.(Microbial infection) Facilitates Dengue virus (DENV) replication, possibly through its action on DDX3X.(Microbial infection) Facilitates Zika virus (ZIKV) replication, possibly through its action on DDX3X.

Alternative names

VRL2, VROAC, VROAC, TRPV4, VRL2, Transient receptor potential cation channel subfamily V member 4, TrpV4, Osm-9-like TRP channel 4, Transient receptor potential protein 12, Vanilloid receptor-like channel 2, Vanilloid receptor-like protein 2, Vanilloid receptor-related osmotically-activated channel, OTRPC4, TRP12, VRL-2, VR-OAC

Recommended products

Rabbit Recombinant Monoclonal TRPV4 antibody. Suitable for WB, IP, IHC-Fr, IHC-P and reacts with Mouse, Human samples. Cited in 1 publication.

Key facts

Isotype

IgG

Form

Liquid

Clonality

Monoclonal

Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number

EPR27068-39

Purification technique

Affinity purification Protein A

Concentration
Loading...

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

TRPV4 also known as transient receptor potential vanilloid 4 is an ion channel protein that plays a mechanical role in the human body. This protein with a mass of approximately 98 kDa is sensitive to osmotic pressure mechanical forces and temperature changes. It acts as a calcium-permeable non-selective cation channel. TRPV4 is widely expressed in various tissues including the choroid plexus as well as in the epithelial cells endothelium and certain neurons. The expression pattern suggests its involvement in various physiological processes.

Biological function summary

TRPV4 serves functions essential for osmoregulation mechanosensation and thermosensation. It often forms a complex with other proteins to modulate calcium influx which influences cellular responses. TRPV4 impacts various biological processes such as maintaining cell volume and adapting to temperature changes. Its role in regulating fluid movement across barriers like those in the choroid plexus demonstrates its involvement in maintaining homeostasis. These interactions highlight the protein's widespread influence on various cellular functions.

Pathways

TRPV4 integrates into critical signal transduction pathways that include the mechanotransduction and osmoregulation pathways. It is closely related to other TRP family members such as TRPV1 and TRPC1 within these pathways. The mechanotransduction pathway allows it to convey mechanical stimuli into electrochemical activity impacting various systems throughout the body. The osmoregulation pathway highlights its ability to sense and respond to osmotic shifts maintaining cellular and systemic equilibrium.

Associated diseases and disorders

TRPV4 has significant links to a range of pathologies such as skeletal dysplasias and neuropathies. Mutations in TRPV4 can lead to altered channel function contributing to conditions like Charcot-Marie-Tooth disease and congenital distal spinal muscular atrophy. These associations often involve the misregulation of calcium homeostasis where TRPV4's interaction with proteins like TRPV1 leads to a disruption of normal cellular processes highlighting the importance of maintaining TRPV4's proper function to prevent those diseases.

Product promise

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Full details and terms and conditions can be found here:
Terms & Conditions.

9 product images

  • Western blot - Anti-TRPV4 antibody [EPR27068-39] (ab307444), expandable thumbnail

    Western blot - Anti-TRPV4 antibody [EPR27068-39] (ab307444)

    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    Negative control: pancreas (PMID:14517216)

    The MW is consistent to what has been described in the literature (PMID:30881453)

    Samples are non-boiled as boiling may cause protein aggregation.

    This blot was developed using a high sensitivity ECL substrate. The high-sensitivity ECL substrate used allows for the detection of proteins in the mid-femtogram range.

    All lanes: Western blot - Anti-TRPV4 antibody [EPR27068-39] (ab307444) at 1/1000 dilution

    Lane 1: Human kidney tissue lysate at 20 µg

    Lane 2: Human pancreas tissue lysate at 20 µg

    Lane 3: Mouse kidney tissue lysate at 20 µg

    Lane 4: Mouse pancreas tissue lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Developed using the ECL technique.

    Observed band size: 100 kDa

    Exposure time: 180s

  • Immunoprecipitation - Anti-TRPV4 antibody [EPR27068-39] (ab307444), expandable thumbnail

    Immunoprecipitation - Anti-TRPV4 antibody [EPR27068-39] (ab307444)

    TRPV4 was immunoprecipitated from 0.35 mg A375 (human malignant melanoma epithelial cell) whole cell lysate 10 ug with ab307444 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab307444 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(VeriBlot for IP Detection Reagent (HRP) ab131366) was used at 1/5000 dilution. Lane 1: A375 (human malignant melanoma epithelial cell) whole cell lysate 10 ug
    Lane 2: ABAB307444 IP in A375 whole cell lysate
    Lane 3:RABbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of ab307444 in A375 whole cell lysate
    Blocking and dilution buffer and concentration: 5% NFDM/TBST.
    Exposure time: 84 seconds

    All lanes: Immunoprecipitation - Anti-TRPV4 antibody [EPR27068-39] (ab307444) at 1/1000 dilution

    Lane 1: A375 (human malignant melanoma epithelial cell) whole cell lysate 10 μg

    Lane 2: A375 whole cell lysate

    Lane 3: Immunoprecipitation - Rabbit IgG, monoclonal [EPR25A] - Isotype Control (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730)

    Secondary

    All lanes: Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366) at 1/5000 dilution

    Observed band size: 100 kDa

    Exposure time: 84s

  • Western blot - Anti-TRPV4 antibody [EPR27068-39] (ab307444), expandable thumbnail

    Western blot - Anti-TRPV4 antibody [EPR27068-39] (ab307444)

    Blocking and diluting buffer and concentration: 5% NFDM/TBSTNegative control: 293T (PMID:29433476)
    Positive control: A375 (PMID:30881453)
    Exposure time: 70 seconds

    All lanes: Western blot - Anti-TRPV4 antibody [EPR27068-39] (ab307444) at 1/1000 dilution

    Lane 1: A375 (human malignant melanoma epithelial cell) whole cell lysate 20 μg

    Lane 2: 293T (human embryonic kidney epithelial cell) whole cell lysate 20 μg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Observed band size: 100 kDa

    Exposure time: 70s

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRPV4 antibody [EPR27068-39] (ab307444), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRPV4 antibody [EPR27068-39] (ab307444)

    Immunohistochemical analysis of paraffin-embedded Mouse kidney tissue lABeling TRPV4 with ab307444 at 1/1000 (0.482 ug/ml) followed by a ready to use LeicaDS9800 (Bond? Polymer Refine Detection) was used. Positive staining on mouse kidney. The section was incubated with ab307444 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND? RX instrument Counterstained with Hematoxylin.
    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond? Polymer Refine Detection) was used.
    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRPV4 antibody [EPR27068-39] (ab307444), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRPV4 antibody [EPR27068-39] (ab307444)

    Immunohistochemical analysis of paraffin-embedded Mouse cerebrum tissue lABeling TRPV4 with ab307444 at 1/1000 (0.482 ug/ml) followed by a ready to use LeicaDS9800 (Bond? Polymer Refine Detection) was used. Positive staining on choroid plexus of mouse cerebrum. The section was incubated with ab307444 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND? RX instrument Counterstained with Hematoxylin.
    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond? Polymer Refine Detection) was used.
    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRPV4 antibody [EPR27068-39] (ab307444), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRPV4 antibody [EPR27068-39] (ab307444)

    Immunohistochemical analysis of paraffin-embedded Human pancreas tissue lABeling TRPV4 with ab307444 at 1/50 (9.64 ug/ml) followed by a ready to use Goat Anti-RABbit IgG H&L (HRP polymer). Negative control: no staining on human pancreas. The section was incubated with ab307444 at 4ЎгC overnight. Counterstained with Hematoxylin.
    Secondary antibody only control: Secondary antibody is a ready to use Goat Anti-RABbit IgG H&L (HRP polymer).
    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRPV4 antibody [EPR27068-39] (ab307444), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRPV4 antibody [EPR27068-39] (ab307444)

    Immunohistochemical analysis of paraffin-embedded Human kidney tissue lABeling TRPV4 with ab307444 at 1/50 (9.64 ug/ml) followed by a ready to use Goat Anti-RABbit IgG H&L (HRP polymer). Positive staining on human kidney. The section was incubated with ab307444 at 4ЎгC overnight. Counterstained with Hematoxylin.
    Secondary antibody only control: Secondary antibody is a ready to use Goat Anti-RABbit IgG H&L (HRP polymer).
    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

  • Immunohistochemistry (Frozen sections) - Anti-TRPV4 antibody [EPR27068-39] (ab307444), expandable thumbnail

    Immunohistochemistry (Frozen sections) - Anti-TRPV4 antibody [EPR27068-39] (ab307444)

    Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeABilized frozen Mouse pancreas (fresh) tissue lABeling TRPV4 with ab307444 at 1/100 (4.82 ug/ml) dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-RABbit IgG H&L (Alexa Fluor? 488) preadsorbed at 1/1000 2 ug/mL dilution (Green). Low expression: confocal image showing no staining on mouse pancreas. The nuclear counterstain was DAPI (Blue). The section was incubated with ab307444 for 60 mins at room temperature. The section was then mounted using Fluoromount?.The immunostaining was performed on a Leica Biosystems BOND? RX instrument. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8). is observed. The nuclear counterstain was DAPI (Blue). Secondary antibody control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-RABbit IgG H&L (Alexa Fluor? 488) preadsorbedat 1/1000 2 ug/mL dilution.

  • Immunohistochemistry (Frozen sections) - Anti-TRPV4 antibody [EPR27068-39] (ab307444), expandable thumbnail

    Immunohistochemistry (Frozen sections) - Anti-TRPV4 antibody [EPR27068-39] (ab307444)

    Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeABilized frozen Mouse kidney (fresh) tissue lABeling TRPV4 with ab307444 at 1/100 (4.82 ug/ml) dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-RABbit IgG H&L (Alexa Fluor? 488) preadsorbed at 1/1000 2 ug/mL dilution (Green). Panel A: merged staining of anti-TRPV4 (ab307444, green) and anti-Nestin (Alexa Fluor® 647 Anti-Nestin antibody [EPR22023] ab306599, red) on mouse kidney.Panel B: anti-TRPV4 stained in the renal tubular system.Panel C: anti-Nestin stained in the glomeruli of kidney.The section was incubated in two rounds of staining: in the order of ab307444 and Alexa Fluor® 647 Anti-Nestin antibody [EPR22023] ab306599 for 1 hr at room temperature. The section was then mounted using Fluoromount?.The immunostaining was performed on a Leica Biosystems BOND? RX instrument. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8). is observed. The nuclear counterstain was DAPI (Blue). Secondary antibody control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-RABbit IgG H&L (Alexa Fluor? 488) preadsorbedat 1/1000 2 ug/mL dilution.

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