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Rabbit Recombinant Multiclonal TRPV4 antibody. Carrier free. Suitable for WB, IP, IHC-P, IHC-Fr and reacts with Human, Mouse, Rat samples.

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Images

Immunohistochemistry (Frozen sections) - Anti-TRPV4 antibody [RM1062] - BSA and Azide free (AB314455), expandable thumbnail
  • Immunohistochemistry (Frozen sections) - Anti-TRPV4 antibody [RM1062] - BSA and Azide free (AB314455), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRPV4 antibody [RM1062] - BSA and Azide free (AB314455), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRPV4 antibody [RM1062] - BSA and Azide free (AB314455), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRPV4 antibody [RM1062] - BSA and Azide free (AB314455), expandable thumbnail

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

pH: 7.2 - 7.4
Constituents: 100% PBS

Form

Liquid

Clonality

Multiclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
WBIPIHC-PIHC-FrFlow CytICC/IF
Human
Tested
Tested
Tested
Expected
Not recommended
Not recommended
Mouse
Tested
Not recommended
Tested
Tested
Not recommended
Not recommended
Rat
Not recommended
Expected
Tested
Expected
Not recommended
Not recommended

Tested
Tested

Species

Human, Mouse

Dilution info

-

Notes

-

Not recommended
Not recommended

Species

Rat

Dilution info

-

Notes

-

Tested
Tested

Species

Human

Dilution info

-

Notes

-

Expected
Expected

Species

Rat

Dilution info

Use at an assay dependent concentration.

Notes

-

Not recommended
Not recommended

Species

Mouse

Dilution info

-

Notes

-

Tested
Tested

Species

Human

Dilution info

-

Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Species

Mouse

Dilution info

-

Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Species

Rat

Dilution info

-

Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Tested
Tested

Species

Mouse

Dilution info

-

Notes

-

Expected
Expected

Species

Human, Rat

Dilution info

Use at an assay dependent concentration.

Notes

-

Not recommended
Not recommended

Species

Human, Mouse, Rat

Dilution info

-

Notes

-

Not recommended
Not recommended

Species

Human, Mouse, Rat

Dilution info

-

Notes

-

Associated Products

Select an associated product type

1 products for Alternative Version

    Target data

    Function

    Non-selective calcium permeant cation channel involved in osmotic sensitivity and mechanosensitivity. Activation by exposure to hypotonicity within the physiological range exhibits an outward rectification (PubMed:18826956, PubMed:18695040, PubMed:29899501). Also activated by heat, low pH, citrate and phorbol esters (PubMed:16293632, PubMed:18826956, PubMed:18695040, PubMed:25256292, PubMed:20037586, PubMed:21964574). Increase of intracellular Ca(2+) potentiates currents. Channel activity seems to be regulated by a calmodulin-dependent mechanism with a negative feedback mechanism (PubMed:12724311, PubMed:18826956). Promotes cell-cell junction formation in skin keratinocytes and plays an important role in the formation and/or maintenance of functional intercellular barriers (By similarity). Acts as a regulator of intracellular Ca(2+) in synoviocytes (PubMed:19759329). Plays an obligatory role as a molecular component in the nonselective cation channel activation induced by 4-alpha-phorbol 12,13-didecanoate and hypotonic stimulation in synoviocytes and also regulates production of IL-8 (PubMed:19759329). Together with PKD2, forms mechano- and thermosensitive channels in cilium (PubMed:18695040). Negatively regulates expression of PPARGC1A, UCP1, oxidative metabolism and respiration in adipocytes (By similarity). Regulates expression of chemokines and cytokines related to proinflammatory pathway in adipocytes (By similarity). Together with AQP5, controls regulatory volume decrease in salivary epithelial cells (By similarity). Required for normal development and maintenance of bone and cartilage (PubMed:26249260). In its inactive state, may sequester DDX3X at the plasma membrane. When activated, the interaction between both proteins is affected and DDX3X relocalizes to the nucleus (PubMed:29899501).Isoform 5Non-selective calcium permeant cation channel involved in osmotic sensitivity and mechanosensitivity. Activation by exposure to hypotonicity within the physiological range exhibits an outward rectification. Also activated by phorbol esters. Has the same channel activity as isoform 1, and is activated by the same stimuli.Isoform 2Lacks channel activity, due to impaired oligomerization and intracellular retention.Isoform 4Lacks channel activity, due to impaired oligomerization and intracellular retention.Isoform 6Lacks channel activity, due to impaired oligomerization and intracellular retention.(Microbial infection) Facilitates hepatitis C virus (HCV) replication, possibly through its action on DDX3X.(Microbial infection) Facilitates Dengue virus (DENV) replication, possibly through its action on DDX3X.(Microbial infection) Facilitates Zika virus (ZIKV) replication, possibly through its action on DDX3X.

    Alternative names

    Transient receptor potential cation channel subfamily V member 4, TrpV4, Osm-9-like TRP channel 4, Transient receptor potential protein 12, Vanilloid receptor-like channel 2, Vanilloid receptor-like protein 2, Vanilloid receptor-related osmotically-activated channel, OTRPC4, TRP12, VRL-2, VR-OAC, VROAC, TRPV4, VRL2

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    Rabbit Recombinant Multiclonal TRPV4 antibody. Carrier free. Suitable for WB, IP, IHC-P, IHC-Fr and reacts with Human, Mouse, Rat samples.

    Alternative names

    Transient receptor potential cation channel subfamily V member 4, TrpV4, Osm-9-like TRP channel 4, Transient receptor potential protein 12, Vanilloid receptor-like channel 2, Vanilloid receptor-like protein 2, Vanilloid receptor-related osmotically-activated channel, OTRPC4, TRP12, VRL-2, VR-OAC, VROAC, TRPV4, VRL2

    Key facts

    Isotype

    IgG

    Form

    Liquid

    Clonality

    Multiclonal

    Immunogens
    • The exact immunogen used to generate this antibody is proprietary information.
    Carrier free

    Yes

    Clone number

    RM1062

    Purification technique

    Affinity purification Protein A

    Specificity

    Unsuitable for mouse IP and rat WB.

    Concentration
    Loading...

    Storage

    Shipped at conditions

    Blue Ice

    Appropriate long-term storage conditions

    +4°C

    Notes

    ab314455 is the carrier-free version of Anti-TRPV4 antibody [RM1062] ab314454.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free batch production

    For more information, read more on recombinant antibodies.

    Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

    This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

    Supplementary info

    Activity summary

    TRPV4 also known as transient receptor potential vanilloid 4 is an ion channel protein that plays a mechanical role in the human body. This protein with a mass of approximately 98 kDa is sensitive to osmotic pressure mechanical forces and temperature changes. It acts as a calcium-permeable non-selective cation channel. TRPV4 is widely expressed in various tissues including the choroid plexus as well as in the epithelial cells endothelium and certain neurons. The expression pattern suggests its involvement in various physiological processes.

    Biological function summary

    TRPV4 serves functions essential for osmoregulation mechanosensation and thermosensation. It often forms a complex with other proteins to modulate calcium influx which influences cellular responses. TRPV4 impacts various biological processes such as maintaining cell volume and adapting to temperature changes. Its role in regulating fluid movement across barriers like those in the choroid plexus demonstrates its involvement in maintaining homeostasis. These interactions highlight the protein's widespread influence on various cellular functions.

    Pathways

    TRPV4 integrates into critical signal transduction pathways that include the mechanotransduction and osmoregulation pathways. It is closely related to other TRP family members such as TRPV1 and TRPC1 within these pathways. The mechanotransduction pathway allows it to convey mechanical stimuli into electrochemical activity impacting various systems throughout the body. The osmoregulation pathway highlights its ability to sense and respond to osmotic shifts maintaining cellular and systemic equilibrium.

    Associated diseases and disorders

    TRPV4 has significant links to a range of pathologies such as skeletal dysplasias and neuropathies. Mutations in TRPV4 can lead to altered channel function contributing to conditions like Charcot-Marie-Tooth disease and congenital distal spinal muscular atrophy. These associations often involve the misregulation of calcium homeostasis where TRPV4's interaction with proteins like TRPV1 leads to a disruption of normal cellular processes highlighting the importance of maintaining TRPV4's proper function to prevent those diseases.

    Product promise

    We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

    In the unlikely event of one of our products not working as expected, you are covered by our product promise.

    Full details and terms and conditions can be found here:
    Terms & Conditions.

    13 product images

    • Immunohistochemistry (Frozen sections) - Anti-TRPV4 antibody [RM1062] - BSA and Azide free (ab314455), expandable thumbnail

      Immunohistochemistry (Frozen sections) - Anti-TRPV4 antibody [RM1062] - BSA and Azide free (ab314455)

      This data was developed using Anti-TRPV4 antibody [RM1062] ab314454, the same antibody clone in a different buffer formulation.
      Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen mouse pancreas (fresh) tissue labeling TRPV4 with Anti-TRPV4 antibody [RM1062] ab314454 at 1/50 (10.08 ug/ml) dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2 ug/mL dilution (Green). Low expression: confocal image showing no staining on mouse pancreas. The nuclear counterstain was DAPI (Blue). The section was incubated with Anti-TRPV4 antibody [RM1062] ab314454 for 60 mins at room temperature. The section was then mounted using Fluoromount®. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8). The nuclear counterstain was DAPI (Blue).
      Secondary antibody control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbedat 1/1000 2 ug/mL dilution.

    • Immunohistochemistry (Frozen sections) - Anti-TRPV4 antibody [RM1062] - BSA and Azide free (ab314455), expandable thumbnail

      Immunohistochemistry (Frozen sections) - Anti-TRPV4 antibody [RM1062] - BSA and Azide free (ab314455)

      This data was developed using Anti-TRPV4 antibody [RM1062] ab314454, the same antibody clone in a different buffer formulation.
      Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Mouse kidney (fresh) tissue labeling TRPV4 with Anti-TRPV4 antibody [RM1062] ab314454 at 1/50 (10.08 ug/ml) dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2 ug/mL dilution (Green). Confocal image showing positive staining on mouse kidney. The nuclear counterstain was DAPI (Blue). The section was incubated with Anti-TRPV4 antibody [RM1062] ab314454 for 60 mins at room temperature. The section was then mounted using Fluoromount®. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8). The nuclear counterstain was DAPI (Blue).
      Secondary antibody control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2 ug/mL dilution.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRPV4 antibody [RM1062] - BSA and Azide free (ab314455), expandable thumbnail

      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRPV4 antibody [RM1062] - BSA and Azide free (ab314455)

      This data was developed using Anti-TRPV4 antibody [RM1062] ab314454, the same antibody clone in a different buffer formulation.
      Immunohistochemical analysis of paraffin-embedded rat skeletal muscle tissue labeling TRPV4 with Anti-TRPV4 antibody [RM1062] ab314454 at 1/1000 (0.504 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Low expression tissue: no staining on rat skeletal muscle. The section was incubated with Anti-TRPV4 antibody [RM1062] ab314454 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
      Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
      Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRPV4 antibody [RM1062] - BSA and Azide free (ab314455), expandable thumbnail

      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRPV4 antibody [RM1062] - BSA and Azide free (ab314455)

      This data was developed using Anti-TRPV4 antibody [RM1062] ab314454, the same antibody clone in a different buffer formulation.
      Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue labeling TRPV4 with Anti-TRPV4 antibody [RM1062] ab314454 at 1/1000 (0.504 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Low expression tissue: weak staining on vascular endothelium of mouse pancreas. The section was incubated with Anti-TRPV4 antibody [RM1062] ab314454 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
      Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
      Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRPV4 antibody [RM1062] - BSA and Azide free (ab314455), expandable thumbnail

      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRPV4 antibody [RM1062] - BSA and Azide free (ab314455)

      This data was developed using Anti-TRPV4 antibody [RM1062] ab314454, the same antibody clone in a different buffer formulation.
      Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue labeling TRPV4 with Anti-TRPV4 antibody [RM1062] ab314454 at 1/1000 (0.504 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Low expression tissue: weak staining on vascular endothelium of mouse skeletal muscle. The section was incubated with Anti-TRPV4 antibody [RM1062] ab314454 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
      Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
      Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRPV4 antibody [RM1062] - BSA and Azide free (ab314455), expandable thumbnail

      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRPV4 antibody [RM1062] - BSA and Azide free (ab314455)

      This data was developed using Anti-TRPV4 antibody [RM1062] ab314454, the same antibody clone in a different buffer formulation.
      Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue labeling TRPV4 with Anti-TRPV4 antibody [RM1062] ab314454 at 1/50 (10.08 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Low expression tissue: no staining on human skeletal muscle. The section was incubated with Anti-TRPV4 antibody [RM1062] ab314454 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
      Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
      Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRPV4 antibody [RM1062] - BSA and Azide free (ab314455), expandable thumbnail

      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRPV4 antibody [RM1062] - BSA and Azide free (ab314455)

      This data was developed using Anti-TRPV4 antibody [RM1062] ab314454, the same antibody clone in a different buffer formulation.
      Immunohistochemical analysis of paraffin-embedded mouse cerebrum tissue labeling TRPV4 with Anti-TRPV4 antibody [RM1062] ab314454 at 1/1000 (0.504 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Positive staining on choroid plexus of mouse cerebrum. The section was incubated with Anti-TRPV4 antibody [RM1062] ab314454 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
      Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
      Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRPV4 antibody [RM1062] - BSA and Azide free (ab314455), expandable thumbnail

      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRPV4 antibody [RM1062] - BSA and Azide free (ab314455)

      This data was developed using Anti-TRPV4 antibody [RM1062] ab314454, the same antibody clone in a different buffer formulation.
      Immunohistochemical analysis of paraffin-embedded mouse cerebrum tissue labeling TRPV4 with Anti-TRPV4 antibody [RM1062] ab314454 at 1/1000 (0.504 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Positive staining on choroid plexus of mouse cerebrum. The section was incubated with Anti-TRPV4 antibody [RM1062] ab314454 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
      Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
      Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRPV4 antibody [RM1062] - BSA and Azide free (ab314455), expandable thumbnail

      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRPV4 antibody [RM1062] - BSA and Azide free (ab314455)

      This data was developed using Anti-TRPV4 antibody [RM1062] ab314454, the same antibody clone in a different buffer formulation.
      Immunohistochemical analysis of paraffin-embedded mouse kidney tissue labeling TRPV4 with Anti-TRPV4 antibody [RM1062] ab314454 at 1/1000 (0.504 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Positive staining on mouse kidney. The section was incubated with Anti-TRPV4 antibody [RM1062] ab314454 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
      Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
      Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRPV4 antibody [RM1062] - BSA and Azide free (ab314455), expandable thumbnail

      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRPV4 antibody [RM1062] - BSA and Azide free (ab314455)

      This data was developed using Anti-TRPV4 antibody [RM1062] ab314454, the same antibody clone in a different buffer formulation.
      Immunohistochemical analysis of paraffin-embedded human kidney tissue labeling TRPV4 with Anti-TRPV4 antibody [RM1062] ab314454 at 1/50 (10.08 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Positive staining on human kidney. The section was incubated with Anti-TRPV4 antibody [RM1062] ab314454 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
      Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
      Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

    • Immunoprecipitation - Anti-TRPV4 antibody [RM1062] - BSA and Azide free (ab314455), expandable thumbnail

      Immunoprecipitation - Anti-TRPV4 antibody [RM1062] - BSA and Azide free (ab314455)

      This data was developed using Anti-TRPV4 antibody [RM1062] ab314454, the same antibody clone in a different buffer formulation.
      TRPV4 was immunoprecipitated from 0.35 mg A375 (human malignant melanoma epithelial cell) whole cell lysate with Anti-TRPV4 antibody [RM1062] ab314454 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using Anti-TRPV4 antibody [RM1062] ab314454 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(VeriBlot for IP Detection Reagent (HRP) ab131366) was used at 1/5000 dilution.
      Lane 1: A375 (human malignant melanoma epithelial cell) whole cell lysate
      Lane 2: Anti-TRPV4 antibody [RM1062] ab314454 IP in A375 (human malignant melanoma epithelial cell) whole cell lysate
      Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of Anti-TRPV4 antibody [RM1062] ab314454 in A375 whole cell lysate
      Blocking and dilution buffer and concentration: 5% NFDM/TBST.

      All lanes: Immunoprecipitation - Anti-TRPV4 antibody [RM1062] (Anti-TRPV4 antibody [RM1062] ab314454) at 1/30 dilution

      All lanes: A375 (human malignant melanoma epithelial cell) whole cell lysate

      Secondary

      All lanes: Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366) at 1/5000 dilution

      Exposure time: 15s

      TRPV4 was immunoprecipitated from 0.35 mg A375 (human malignant melanoma epithelial cell) whole cell lysate with Anti-TRPV4 antibody [RM1062] ab314454 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using Anti-TRPV4 antibody [RM1062] ab314454 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(VeriBlot for IP Detection Reagent (HRP) ab131366) was used at 1/5000 dilution.
      Lane 1: A375 (human malignant melanoma epithelial cell) whole cell lysate
      Lane 2: Anti-TRPV4 antibody [RM1062] ab314454 IP in A375 (human malignant melanoma epithelial cell) whole cell lysate
      Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of Anti-TRPV4 antibody [RM1062] ab314454 in A375 whole cell lysate
      Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    • Western blot - Anti-TRPV4 antibody [RM1062] - BSA and Azide free (ab314455), expandable thumbnail

      Western blot - Anti-TRPV4 antibody [RM1062] - BSA and Azide free (ab314455)

      This data was developed using Anti-TRPV4 antibody [RM1062] ab314454, the same antibody clone in a different buffer formulation.
      Blocking and diluting buffer and concentration: 5% NFDM/TBST
      Low expression: skeletal muscle and pancreas (PMID:16627472; PMID: 14517216).
      In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.

      All lanes: Western blot - Anti-TRPV4 antibody [RM1062] (Anti-TRPV4 antibody [RM1062] ab314454) at 1/1000 dilution

      Lane 1: Mouse kidney tissue lysate at 20 µg

      Lane 2: Mouse skeletal muscle tissue lysate at 20 µg

      Lane 3: Mouse pancreas tissue lysate at 20 µg

      Secondary

      All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution

      Observed band size: 98 kDa

      Exposure time: 59s

      Blocking and diluting buffer and concentration: 5% NFDM/TBST
      Low expression: skeletal muscle and pancreas (PMID:16627472; PMID: 14517216).
      In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.

    • Western blot - Anti-TRPV4 antibody [RM1062] - BSA and Azide free (ab314455), expandable thumbnail

      Western blot - Anti-TRPV4 antibody [RM1062] - BSA and Azide free (ab314455)

      This data was developed using Anti-TRPV4 antibody [RM1062] ab314454, the same antibody clone in a different buffer formulation.
      Blocking and diluting buffer and concentration: 5% NFDM/TBST
      Low expression: 293T (PMID:29433476) and skeletal muscle (PMID:16627472).
      Samples are non-boiled as boiling may cause protein aggregation.
      In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.

      All lanes: Western blot - Anti-TRPV4 antibody [RM1062] (Anti-TRPV4 antibody [RM1062] ab314454) at 1/1000 dilution

      Lane 1: Human kidney tissue lysate at 20 µg

      Lane 2: Human skeletal muscle tissue lysate at 20 µg

      Lane 3: A375 (human malignant melanoma epithelial cell) whole cell lysate at 20 µg

      Lane 4: 293T (human embryonic kidney epithelial cell) whole cell lysate at 20 µg

      Secondary

      All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution

      Observed band size: 98 kDa

      Exposure time: 59s

      Blocking and diluting buffer and concentration: 5% NFDM/TBST
      Low expression: 293T (PMID:29433476) and skeletal muscle (PMID:16627472).
      Samples are non-boiled as boiling may cause protein aggregation.
      In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.

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