AB227590

Anti-TRRAP antibody

Name: Anti-TRRAP antibody (ab227590) | Abcam
Brand: Abcam Limited
SKU: AB227590
Rating: 3 (1 reviews)

(1 Review)

(1 Publication)

Rabbit Polyclonal TRRAP antibody. Suitable for IP, ChIP, WB and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human TRRAP.

View Alternative Names

PAF400, TRRAP, Transformation/transcription domain-associated protein, 350/400 kDa PCAF-associated factor, STAF40, Tra1 homolog, PAF350/400

4 Images

Supplier Data

Immunoprecipitation - Anti-TRRAP antibody (AB227590)

TRRAP was immunoprecipitated from HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell extract with 4 μg of ab227590. Western blot was performed from the immunoprecipitate using ab227590 at 1/1000 dilution. Anti-rabbit IgG was used as a secondary reagent.

Lane 1 : 4 μg of preimmune Rabbit IgG instead of ab227590 in HEK-293T whole cell extract.
Lane 2 : ab227590 IP in HEK-293T whole cell extract.

5% SDS-PAGE gel.

All lanes:

Immunoprecipitation - Anti-TRRAP antibody (ab227590)

Predicted band size: 438 kDa

false

ChIP

Supplier Data

ChIP - Anti-TRRAP antibody (AB227590)

Cross-linked ChIP was performed with HeLa (human epithelial cell line from cervix adenocarcinoma) chromatin extract and 5 μg of either control rabbit IgG or ab227590. The precipitated DNA was detected by PCR with primer set targeting to nucleolin promoter.

Supplier Data

Western blot - Anti-TRRAP antibody (AB227590)

5% SDS-PAGE gel.

All lanes:

Western blot - Anti-TRRAP antibody (ab227590) at 1/1000 dilution

Lane 1:

Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate at 30 µg

Lane 2:

Raji (human Burkitt's lymphoma cell line) whole cell lysate at 30 µg

Lane 3:

NCI-H929 (human bone marrow lymphoblast cell line) whole cell lysate at 30 µg

Predicted band size: 438 kDa

false

Supplier Data

Western blot - Anti-TRRAP antibody (AB227590)

5% SDS-PAGE gel.

All lanes:

Western blot - Anti-TRRAP antibody (ab227590) at 1/1000 dilution

Lane 1:

HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 30 µg

Lane 2:

A431 (human epidermoid carcinoma cell line) whole cell lysate at 30 µg

Lane 3:

HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 30 µg

Lane 4:

HepG2 (human liver hepatocellular carcinoma cell line) whole cell lysate at 30 µg

Predicted band size: 438 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

ChIP, IP, WB

applications

Immunogen

Recombinant Fragment Protein within Human TRRAP. The exact immunogen used to generate this antibody is proprietary information.

Q9Y4A5

style

left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "ChIP" : {"fullname" : "ChIP", "shortname":"ChIP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/100 - 1/500", "IP-species-notes": "", "ChIP-species-checked": "testedAndGuaranteed", "ChIP-species-dilution-info": "", "ChIP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "" }, "Mouse": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "ChIP-species-checked": "predicted", "ChIP-species-dilution-info": "", "ChIP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rat": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "ChIP-species-checked": "predicted", "ChIP-species-dilution-info": "", "ChIP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Cow": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "ChIP-species-checked": "predicted", "ChIP-species-dilution-info": "", "ChIP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

style

left-column

style

left-column

Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7 Preservative: 0.025% Proclin 300 Constituents: PBS, 20% Glycerol (glycerin, glycerine)

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

style

left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The TRRAP (Transformation/Transcription Domain-Associated Protein) also known simply as TRAAP acts as an adaptor protein with a molecular weight of approximately 434 kDa. TRRAP is widely expressed across different tissue types playing a significant role in cellular processes. As part of a larger family of proteins linked to phosphoinositide 3-kinase-related kinases TRRAP does not perform any kinase activity despite having a structure similar to these kinases. Its primary function involves facilitating chromatin remodeling and histone acetylation.

Biological function summary

TRRAP functions as part of several multiprotein acetyltransferase complexes notably the Tip60 and SAGA complexes. These complexes modify chromatin structures affecting transcription by acetylating histone proteins which allows genes to be more accessible for transcription. This role is important for regulating gene expression making TRRAP important in cellular differentiation and proliferation. By interacting with specific compartments of the cell it participates in large chromatin-modifying machines that influence cellular outcomes.

Pathways

You will find TRRAP involved in pivotal biological routes such as the DNA damage response and Wnt signaling. In the DNA damage response pathway TRRAP interacts with Histone H4 and influences DNA repair mechanisms. TRRAP also collaborates with proteins like c-MYC in these signaling networks impacting both gene transcription and cell cycle progression. Through its associations with these pathways TRRAP assists in processes that maintain genome stability contributing to normal and pathological cellular states.

TRRAP demonstrates a notable connection to cancer and neurological disorders. Mutations and aberrations in TRRAP expression levels have associations with various cancers including breast and colorectal tumors likely due to its influence on cell proliferation and survival. In the context of neurological disorders deficient TRRAP activity might relate to problems in neural development further highlighting its interaction with c-MYC in maintaining proper cellular growth balance. Understanding TRRAP's function and interactions can offer valuable insights into its role in disease mechanisms.

style

left-column

style

left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

style

left-column

Target data

Adapter protein, which is found in various multiprotein chromatin complexes with histone acetyltransferase activity (HAT), which gives a specific tag for epigenetic transcription activation. Component of the NuA4 histone acetyltransferase complex which is responsible for acetylation of nucleosomal histones H4 and H2A. Plays a central role in MYC transcription activation, and also participates in cell transformation by MYC. Required for p53/TP53-, E2F1- and E2F4-mediated transcription activation. Also involved in transcription activation mediated by the adenovirus E1A, a viral oncoprotein that deregulates transcription of key genes. Probably acts by linking transcription factors such as E1A, MYC or E2F1 to HAT complexes such as STAGA thereby allowing transcription activation. Probably not required in the steps following histone acetylation in processes of transcription activation. May be required for the mitotic checkpoint and normal cell cycle progression. Component of a SWR1-like complex that specifically mediates the removal of histone H2A.Z/H2AZ1 from the nucleosome. May play a role in the formation and maintenance of the auditory system (By similarity).

See full target information TRRAP

style

left-column

Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Cell transplantation 31:9636897221081483 PubMed35236160

2022

The Roles of Different Multigene Combinations of , , , , and in the Reprogramming of Canine ADSCs Into IPCs.

Applications

Unspecified application

Species

Unspecified reactive species

Dengke Gao,Pengxiu Dai,Zhixin Fan,Jinglu Wang,Yihua Zhang

View all publications

style

left-column

style

left-column

style

right-column