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AB244240

Anti-TRUB2 antibody [EPR17326] - BSA and Azide free

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(1 Publication)

Rabbit Recombinant Monoclonal TRUB2 antibody. Carrier free. Suitable for WB and reacts with Human samples. Cited in 1 publication.

View Alternative Names

TruB pseudouridine synthase homolog 2, tRNA pseudouridine 55 synthase TRUB2, Psi55 synthase TRUB2, TRUB2

2 Images
Western blot - Anti-TRUB2 antibody [EPR17326] - BSA and Azide free (AB244240)
  • WB

Supplier Data

Western blot - Anti-TRUB2 antibody [EPR17326] - BSA and Azide free (AB244240)

This data was developed using ab199731, the same antibody clone in a different buffer formulation.

Blocking and dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-TRUB2 antibody [EPR17326] (<a href='/en-us/products/primary-antibodies/trub2-antibody-epr17326-ab199731'>ab199731</a>) at 1/5000 dilution

Lane 1:

A549 (Human lung carcinoma) whole cell lysate at 20 µg

Lane 2:

HepG2 (Human liver hepatocellular carcinoma) whole cell lysate at 20 µg

Lane 3:

K562 (Human chronic myelogenous leukemia cells from bone marrow) whole cell lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 37 kDa

Observed band size: 37 kDa

false

Exposure time: 10s

Western blot - Anti-TRUB2 antibody [EPR17326] - BSA and Azide free (AB244240)
  • WB

Supplier Data

Western blot - Anti-TRUB2 antibody [EPR17326] - BSA and Azide free (AB244240)

This data was developed using ab199731, the same antibody clone in a different buffer formulation.

Blocking and dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-TRUB2 antibody [EPR17326] (<a href='/en-us/products/primary-antibodies/trub2-antibody-epr17326-ab199731'>ab199731</a>) at 1/1000 dilution

All lanes:

Human fetal skin lysate at 10 µg

Secondary

All lanes:

Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

Predicted band size: 37 kDa

Observed band size: 37 kDa

false

Exposure time: 3min

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR17326

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>" } } }
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Product details

ab244240 is the carrier-free version of ab199731.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

TRUB2 also known as PUS9 or Pseudouridylate Synthase 9 is an enzyme with a mass of approximately 60 kDa. It is a mitochondrial protein involved in catalyzing the conversion of uridine to pseudouridine in RNA molecules specifically tRNA. Expression of TRUB2 occurs mainly in the mitochondrial matrix of cells where it plays an important role in maintaining proper mitochondrial RNA structure and function.
Biological function summary

TRUB2 impacts RNA stability and processing within mitochondria. It forms part of a complex that modifies RNA to maintain accurate mitochondrial translation. This modification is essential for correct folding and functioning of tRNA impacting the synthesis of proteins required for efficient energy production in cells. TRUB2 ensures that mitochondrial RNA maintains a stable structure facilitating the cellular energy needs through proper protein synthesis.

Pathways

TRUB2 integrates into mitochondrial biogenesis and protein synthesis pathways. It works in mitochondrial translation a critical biological process for cellular energy production. TRUB2 interacts with mitochondrial ribosomal proteins and other enzymes like AARS2 which are associated with aminoacylation and further tRNA modification ensuring efficient protein synthesis. This positioning in the pathway demonstrates its role in sustaining mitochondrial function and overall cellular energy homeostasis.

TRUB2 has associations with mitochondrial dysfunction-related diseases such as mitochondrial encephalomyopathy and respiratory chain disorders. Mutations in TRUB2 can disrupt mitochondrial RNA modification leading to defective protein synthesis and impaired energy metabolism. Its interaction with proteins such as AARS2 also implicates it in mitochondrial myopathy where both play roles in defective tRNA modification. Understanding TRUB2's role helps in exploring therapeutic approaches for linked mitochondrial disorders.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Minor enzyme contributing to the isomerization of uridine to pseudouridine (pseudouridylation) of specific mitochondrial mRNAs (mt-mRNAs) such as COXI and COXIII mt-mRNAs (PubMed : 27974379, PubMed : 31477916). As a component of a functional protein-RNA module, consisting of RCC1L, NGRN, RPUSD3, RPUSD4, TRUB2, FASTKD2 and 16S mitochondrial ribosomal RNA (16S mt-rRNA), controls 16S mt-rRNA abundance and is required for intra-mitochondrial translation (PubMed : 27667664). Also catalyzes pseudouridylation of some tRNAs, including synthesis of pseudouridine(55) from uracil-55, in the psi GC loop of a subset of tRNAs (PubMed : 33023933).
See full target information TRUB2
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

iScience 28:112992 PubMed40687785

2025

Differential substrate degradation by super-degron: EGFP in wild-type mouse cells, PD-1 requires CRBN humanization.

Applications

Unspecified application

Species

Unspecified reactive species

Chie Naruse,Ojiro Ishibashi,Masatoshi Ohgushi,Hirohiko Imai,Tomoko Matsuzaki,Xuchi Pan,Tatsuhiko Miyazaki,Yuka Shidahara,Yu Shirakawa,Fumihiro Sugiyama,Masahide Asano
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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