AB17263

Anti-Trypsin antibody [2C4]

Name: Anti-Trypsin antibody [2C4] (ab17263) | Abcam
Brand: Abcam Limited
SKU: AB17263

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(3 Publications)

Mouse Monoclonal Trypsin antibody. Suitable for WB and reacts with Human samples. Cited in 3 publications. Immunogen corresponding to Native Full Length Protein corresponding to Human PRSS1.

View Alternative Names

TRP1, TRY1, TRYP1, PRSS1, Serine protease 1, Anionic trypsin I, Anionic trypsin-I, Beta-trypsin, Cationic trypsinogen, Pretrypsinogen I, Trypsin I, Trypsin-1

1 Images

Unknown

Western blot - Anti-Trypsin antibody [2C4] (AB17263)

All lanes:

Western blot - Anti-Trypsin antibody [2C4] (ab17263) at 5 µg/mL

Lane 1:

Western blot - Native human Trypsin protein (<a href='/en-us/products/proteins-peptides/native-human-trypsin-protein-ab78527'>ab78527</a>) at 1 µg

Lane 2:

Western blot - Native human Trypsin protein (<a href='/en-us/products/proteins-peptides/native-human-trypsin-protein-ab78527'>ab78527</a>) at 0.1 µg

Secondary

All lanes:

Western blot - Goat Anti-Mouse IgG H&L (HRP) preadsorbed (<a href='/en-us/products/secondary-antibodies/goat-mouse-igg-h-l-hrp-preadsorbed-ab97040'>ab97040</a>) at 1/5000 dilution

Predicted band size: 27 kDa

true

Exposure time: 20min

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

2C4

Isotype

IgG1

Light chain type

kappa

Carrier free

Reacts with

Human

Applications

applications

Immunogen

Native Full Length Protein corresponding to Human PRSS1.

P07477

Specificity

This antibody is specific for human trypsin, trypsinogen and trypsin in complex with alpha 1 antitrypsin.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein G

Storage buffer

pH: 7.4 Preservative: 0.097% Sodium azide Constituents: PBS, 2.9% Sodium chloride

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Trypsin is a serine protease enzyme with a molecular mass around 24 kDa. Known also as "bovine trypsin" or "pancreatic trypsin" it functions primarily in the digestive system. Trypsin hydrolyzes peptide bonds specifically at the carboxyl side of the amino acids lysine and arginine. The enzyme is produced in the pancreas as an inactive precursor trypsinogen which converts to active trypsin in the small intestine.

Biological function summary

Trypsin breaks down proteins into smaller peptides facilitating digestion. It is not part of a complex but interacts with other pancreatic enzymes like chymotrypsin and elastase. Trypsin also plays a regulatory role in activating other zymogens like procarboxypeptidase and chymotrypsinogen essential in the complete protein digestion process.

Pathways

The catalytic actions of trypsin are integral to the digestive pathway in the gastrointestinal tract. It ensures the efficient breakdown and absorption of nutrients. In the trypsin activation pathway it activates other proteases which are significant for protein catabolism. This enzyme collaborates closely with anti-trypsin inhibitors that regulate its activity preventing autodigestion and maintaining homeostasis.

Trypsin relates closely to pancreatitis and cystic fibrosis. Unregulated activation of trypsinogen within the pancreas causes pancreatitis a condition characterized by inflammation and damage to pancreatic cells. Alpha-1 anti-trypsin an important inhibitor of trypsin connects to conditions like emphysema and liver disease due to its dysfunctional regulation indicating a broader implication of trypsin in maintaining cellular health.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Has activity against the synthetic substrates Boc-Phe-Ser-Arg-Mec, Boc-Leu-Thr-Arg-Mec, Boc-Gln-Ala-Arg-Mec and Boc-Val-Pro-Arg-Mec. The single-chain form is more active than the two-chain form against all of these substrates.

See full target information PRSS1

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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

American journal of cancer research 13:2714-2731 PubMed37424812

2023

Long non-coding RNA enhances gastric carcinoma proliferation, migration and invasion via the miR-136-5p/HOXC10 axis.

Applications

Unspecified application

Species

Unspecified reactive species

Jingzhang Li,Kai Sun,Mingming Zhang,Yusheng Luo,Ying Zhang,Cheng Huang,Juanjuan Hong,Dongmei Chen,Wangjun Liao

The Analyst 145:5801-5807 PubMed32692324

2020

Picoliter enzyme reactor on a nanofluidic device exceeding the bulk reaction rate.

Applications

Unspecified application

Species

Unspecified reactive species

Koki Yamamoto,Kyojiro Morikawa,Hiroyuki Imanaka,Koreyoshi Imamura,Takehiko Kitamori

Pharmaceutical research 29:332-41 PubMed21818710

2011

Investigations into the fate of inhaled salmon calcitonin at the respiratory epithelial barrier.

Applications

Unspecified application

Species

Unspecified reactive species

Leonie Baginski,Frederic Tewes,Stephen T Buckley,Anne Marie Healy,Udo Bakowsky,Carsten Ehrhardt

View all publications

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