Anti-Trypsin antibody [EPR19497]

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Trypsin antibody [EPR19497] (AB200996)

Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling PRSS1 with ab200996 at 1/8000 dilution followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Negative staining on Human kidney. Counter stained with Hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody secondary antibody is ab97051 at 1/500 dilution.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Trypsin antibody [EPR19497] (AB200996)

Immunohistochemical analysis of paraffin-embedded Human pancreas tissue labeling PRSS1 with ab200996 at 1/8000 dilution followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Cytoplasm staining on acinar cells Human pancreas is observed. Counter stained with Hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

• WB

Supplier Data

Western blot - Anti-Trypsin antibody [EPR19497] (AB200996)

Blocking/Dilution buffer : 5% NFDM/TBST.

Trypsin 1, encoded by the PRSS1 gene, is synthesized in the pancreas. The expression profile observed is consistent with what has been described in the literatures (PMID : 3011602, 7945238, 24722290).

All lanes:

Western blot - Anti-Trypsin antibody [EPR19497] (ab200996) at 1/5000 dilution

Lane 1:

Human pancreas lysate at 10 µg

Lane 2:

Human fetal liver tissue lysate at 10 µg

Lane 3:

Human fetal heart tissue lysate at 10 µg

Lane 4:

Human fetal kidney tissue lysate at 10 µg

Lane 5:

Human fetal spleen tissue lysate at 10 µg

Lane 6:

Human fetal lung tissue lysate at 10 µg

Lane 7:

BxPC-3 (Human pancreas adenocarcinoma cell line) whole cell lysate at 10 µg

Lane 8:

THP-1 (Human monocytic leukemia cell line) whole cell lysate at 10 µg

Lane 9:

HT-29 (Human colorectal adenocarcinoma cell line) whole cell lysate at 10 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG Peroxidase Conjugate, specific to the non-reduced form of IgG at 1/10000 dilution

Predicted band size: 27 kDa

Observed band size: 27 kDa

false

Exposure time: 3min

• WB

Supplier Data

Western blot - Anti-Trypsin antibody [EPR19497] (AB200996)

Blocking/Dilution buffer : 5% NFDM/TBST.

Exposure time : Lane 1 : 1 second; Lane 2 and 3 : 3 minutes.

Human PRSS1 fragment recombinant protein contains aa24-122 with a His-Tag^®. Human TRY3 full length recombinant protein contains aa1-304 with a His-Tag^®. Human TRY6 full length recombinant protein contains aa1-247 with a His-Tag^®. These three recombinant proteins were made in-house.

All lanes:

Western blot - Anti-Trypsin antibody [EPR19497] (ab200996) at 1/1000 dilution

Lane 1:

Human PRSS1 fragment recombinant protein at 0.01 µg

Lane 2:

Human TRY3 full length recombinant protein at 0.01 µg

Lane 3:

Human TRY6 full length recombinant protein at 0.01 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 27 kDa

false

• WB

Supplier Data

Western blot - Anti-Trypsin antibody [EPR19497] (AB200996)

Blocking/Dilution buffer : 5% NFDM/TBST.

Human TRY2 full length recombinant protein contains aa1-247 with a GST-Tag. It was made in-house.

All lanes:

Western blot - Anti-Trypsin antibody [EPR19497] (ab200996) at 1/1000 dilution

All lanes:

Human TRY2 full length recombinant protein at 0.01 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 27 kDa

false

Exposure time: 3min