Anti-Trypsin antibody [EPR19498-43]

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Trypsin antibody [EPR19498-43] (AB200997)

Immunohistochemical analysis of paraffin-embedded Human colon tissue labeling Trypsin with ab200997 at 1/2000 dilution followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Negative staining on Human colon. Counter stained with Hematoxylin.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Trypsin antibody [EPR19498-43] (AB200997)

Immunohistochemical analysis of paraffin-embedded Human pancreas cancer tissue labeling Trypsin with ab200997 at 1/2000 dilution followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Cytoplasm staining on Human pancreas cancer is observed. Counter stained with Hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Trypsin antibody [EPR19498-43] (AB200997)

Immunohistochemical analysis of paraffin-embedded Human cerebrum tissue labeling Trypsin with ab200997 at 1/2000 dilution followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Cytoplasm staining on Human cerebrum is observed. Counter stained with Hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Trypsin antibody [EPR19498-43] (AB200997)

Immunohistochemical analysis of paraffin-embedded Human pancreas tissue labeling Trypsin with ab200997 at 1/2000 dilution followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Cytoplasm staining on acinar cells of Human pancreas is observed. Counter stained with Hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

• WB

Supplier Data

Western blot - Anti-Trypsin antibody [EPR19498-43] (AB200997)

Blocking/Dilution buffer : 5% NFDM/TBST.

Exposure time : Lane 1 : 1 second; Lane 2 : 3 minutes.

Human PRSS1 fragment recombinant protein contains aa123-247 with a GST-Tag; Human PRSS2 full length recombinant protein contains aa1-247 with a GST-Tag. These two recombinant proteins were made in-house.

All lanes:

Western blot - Anti-Trypsin antibody [EPR19498-43] (ab200997) at 1/1000 dilution

Lane 1:

Human PRSS1 fragment recombinant protein at 0.01 µg

Lane 2:

Human PRSS2 full length recombinant protein at 0.01 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 27 kDa

false

• WB

Supplier Data

Western blot - Anti-Trypsin antibody [EPR19498-43] (AB200997)

Blocking/Dilution buffer : 5% NFDM/TBST.

Trypsinogens I (PRSS1), Trypsinogens II (PRSS2), and mesotrypsinogen (PRSS3) are the major trypsinogen genes in the human pancreas. Trypsinogens I and II are the principal forms of trypsin that are secreted in pancreatic juice, while mesotrypsinogen is a minor trypsinogen in human pancreatic juice [PMID : 14726524, 3011602, 6698368, and 9099703]. This product recognizes PRSS1, and shows some cross-reactivity with PRSS3 and PRSS3P2 proteins.

All lanes:

Western blot - Anti-Trypsin antibody [EPR19498-43] (ab200997) at 1/2000 dilution

Lane 1:

Human pancreas tissue lysate at 10 µg

Lane 2:

Human fetal liver tissue lysate at 10 µg

Lane 3:

Human fetal heart tissue lysate at 10 µg

Lane 4:

Human fetal kidney tissue lysate at 10 µg

Lane 5:

Human fetal spleen tissue lysate at 10 µg

Lane 6:

Human fetal lung tissue lysate at 10 µg

Lane 7:

BxPC-3 (Human pancreas adenocarcinoma cell line) whole cell lysate at 10 µg

Lane 8:

THP-1 (Human monocytic leukemia cell line) whole cell lysate at 10 µg

Lane 9:

HT-29 (Human colorectal adenocarcinoma cell line) whole cell lysate at 10 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG Peroxidase Conjugate, specific to the non-reduced form of IgG at 1/10000 dilution

Predicted band size: 27 kDa

Observed band size: 27 kDa

false

Exposure time: 3min

• WB

Supplier Data

Western blot - Anti-Trypsin antibody [EPR19498-43] (AB200997)

Blocking/Dilution buffer : 5% NFDM/TBST.

Human PRSS3 full length recombinant protein contains aa1-304 with a His-Tag®. Human PRSS3P2 full length recombinant protein contains aa1-247 with a His-Tag®. These two recombinant proteins were made in-house.

All lanes:

Western blot - Anti-Trypsin antibody [EPR19498-43] (ab200997) at 1/1000 dilution

Lane 1:

Human PRSS3 full length recombinant protein at 0.01 µg

Lane 2:

Human PRSS3P2 full length recombinant protein at 0.01 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 27 kDa

Observed band size: 27 kDa

false

Exposure time: 3min