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Rabbit Recombinant Monoclonal Trypsin antibody. Suitable for WB, IHC-P and reacts with Human samples. Cited in 5 publications.


Images

Western blot - Anti-Trypsin antibody [EPR19498-43] (AB200997), expandable thumbnail
  • Western blot - Anti-Trypsin antibody [EPR19498-43] (AB200997), expandable thumbnail
  • Western blot - Anti-Trypsin antibody [EPR19498-43] (AB200997), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Trypsin antibody [EPR19498-43] (AB200997), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Trypsin antibody [EPR19498-43] (AB200997), expandable thumbnail

Publications

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
WBIHC-P
Human
Tested
Tested

Tested
Tested

Species
Human
Dilution info
1/1000
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Tested
Tested

Species
Human
Dilution info
1/2000
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Associated Products

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3 products for Alternative Product

Target data

Function

Has activity against the synthetic substrates Boc-Phe-Ser-Arg-Mec, Boc-Leu-Thr-Arg-Mec, Boc-Gln-Ala-Arg-Mec and Boc-Val-Pro-Arg-Mec. The single-chain form is more active than the two-chain form against all of these substrates.

Alternative names

TRP1, TRY1, TRYP1, PRSS1, Serine protease 1, Anionic trypsin I, Anionic trypsin-I, Beta-trypsin, Cationic trypsinogen, Pretrypsinogen I, Trypsin I, Trypsin-1

Recommended products

Rabbit Recombinant Monoclonal Trypsin antibody. Suitable for WB, IHC-P and reacts with Human samples. Cited in 5 publications.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
EPR19498-43
Purification technique
Affinity purification Protein A
Specificity

This product reacts with human Trypsin 1, Trypsin 3 and Putative trypsin 6 but not with Trypsin 2. However, it might cross react with other family members and isoforms.

Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

Trypsin is a serine protease enzyme with a molecular mass around 24 kDa. Known also as "bovine trypsin" or "pancreatic trypsin" it functions primarily in the digestive system. Trypsin hydrolyzes peptide bonds specifically at the carboxyl side of the amino acids lysine and arginine. The enzyme is produced in the pancreas as an inactive precursor trypsinogen which converts to active trypsin in the small intestine.

Biological function summary

Trypsin breaks down proteins into smaller peptides facilitating digestion. It is not part of a complex but interacts with other pancreatic enzymes like chymotrypsin and elastase. Trypsin also plays a regulatory role in activating other zymogens like procarboxypeptidase and chymotrypsinogen essential in the complete protein digestion process.

Pathways

The catalytic actions of trypsin are integral to the digestive pathway in the gastrointestinal tract. It ensures the efficient breakdown and absorption of nutrients. In the trypsin activation pathway it activates other proteases which are significant for protein catabolism. This enzyme collaborates closely with anti-trypsin inhibitors that regulate its activity preventing autodigestion and maintaining homeostasis.

Associated diseases and disorders

Trypsin relates closely to pancreatitis and cystic fibrosis. Unregulated activation of trypsinogen within the pancreas causes pancreatitis a condition characterized by inflammation and damage to pancreatic cells. Alpha-1 anti-trypsin an important inhibitor of trypsin connects to conditions like emphysema and liver disease due to its dysfunctional regulation indicating a broader implication of trypsin in maintaining cellular health.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

7 product images

  • Western blot - Anti-Trypsin antibody [EPR19498-43] (ab200997), expandable thumbnail

    Western blot - Anti-Trypsin antibody [EPR19498-43] (ab200997)

    Blocking/Dilution buffer: 5% NFDM/TBST.

    Exposure time: Lane 1: 1 second; Lane 2: 3 minutes.

    Human PRSS1 fragment recombinant protein contains aa123-247 with a GST-Tag; Human PRSS2 full length recombinant protein contains aa1-247 with a GST-Tag. These two recombinant proteins were made in-house.

    All lanes: Western blot - Anti-Trypsin antibody [EPR19498-43] (ab200997) at 1/1000 dilution

    Lane 1: Human PRSS1 fragment recombinant protein at 0.01 µg

    Lane 2: Human PRSS2 full length recombinant protein at 0.01 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Predicted band size: 27 kDa

  • Western blot - Anti-Trypsin antibody [EPR19498-43] (ab200997), expandable thumbnail

    Western blot - Anti-Trypsin antibody [EPR19498-43] (ab200997)

    Blocking/Dilution buffer: 5% NFDM/TBST.

    Human PRSS3 full length recombinant protein contains aa1-304 with a His-Tag®. Human PRSS3P2 full length recombinant protein contains aa1-247 with a His-Tag®. These two recombinant proteins were made in-house.

    All lanes: Western blot - Anti-Trypsin antibody [EPR19498-43] (ab200997) at 1/1000 dilution

    Lane 1: Human PRSS3 full length recombinant protein at 0.01 µg

    Lane 2: Human PRSS3P2 full length recombinant protein at 0.01 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Predicted band size: 27 kDa

    Observed band size: 27 kDa

    Exposure time: 3min

  • Western blot - Anti-Trypsin antibody [EPR19498-43] (ab200997), expandable thumbnail

    Western blot - Anti-Trypsin antibody [EPR19498-43] (ab200997)

    Blocking/Dilution buffer: 5% NFDM/TBST.

    Trypsinogens I (PRSS1), Trypsinogens II (PRSS2), and mesotrypsinogen (PRSS3) are the major trypsinogen genes in the human pancreas. Trypsinogens I and II are the principal forms of trypsin that are secreted in pancreatic juice, while mesotrypsinogen is a minor trypsinogen in human pancreatic juice [PMID: 14726524, 3011602, 6698368, and 9099703]. This product recognizes PRSS1, and shows some cross-reactivity with PRSS3 and PRSS3P2 proteins.

    All lanes: Western blot - Anti-Trypsin antibody [EPR19498-43] (ab200997) at 1/2000 dilution

    Lane 1: Human pancreas tissue lysate at 10 µg

    Lane 2: Human fetal liver tissue lysate at 10 µg

    Lane 3: Human fetal heart tissue lysate at 10 µg

    Lane 4: Human fetal kidney tissue lysate at 10 µg

    Lane 5: Human fetal spleen tissue lysate at 10 µg

    Lane 6: Human fetal lung tissue lysate at 10 µg

    Lane 7: BxPC-3 (Human pancreas adenocarcinoma cell line) whole cell lysate at 10 µg

    Lane 8: THP-1 (Human monocytic leukemia cell line) whole cell lysate at 10 µg

    Lane 9: HT-29 (Human colorectal adenocarcinoma cell line) whole cell lysate at 10 µg

    Secondary

    All lanes: Goat Anti-Rabbit IgG Peroxidase Conjugate, specific to the non-reduced form of IgG at 1/10000 dilution

    Predicted band size: 27 kDa

    Observed band size: 27 kDa

    Exposure time: 3min

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Trypsin antibody [EPR19498-43] (ab200997), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Trypsin antibody [EPR19498-43] (ab200997)

    Immunohistochemical analysis of paraffin-embedded Human pancreas tissue labeling Trypsin with ab200997 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/500 dilution. Cytoplasm staining on acinar cells of Human pancreas is observed. Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/500 dilution.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Trypsin antibody [EPR19498-43] (ab200997), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Trypsin antibody [EPR19498-43] (ab200997)

    Immunohistochemical analysis of paraffin-embedded Human cerebrum tissue labeling Trypsin with ab200997 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/500 dilution. Cytoplasm staining on Human cerebrum is observed. Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/500 dilution.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Trypsin antibody [EPR19498-43] (ab200997), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Trypsin antibody [EPR19498-43] (ab200997)

    Immunohistochemical analysis of paraffin-embedded Human colon tissue labeling Trypsin with ab200997 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/500 dilution. Negative staining on Human colon. Counter stained with Hematoxylin.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Trypsin antibody [EPR19498-43] (ab200997), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Trypsin antibody [EPR19498-43] (ab200997)

    Immunohistochemical analysis of paraffin-embedded Human pancreas cancer tissue labeling Trypsin with ab200997 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/500 dilution. Cytoplasm staining on Human pancreas cancer is observed. Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/500 dilution.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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