AB52954

Anti-Tryptophan Hydroxylase/TPH antibody [EP1311Y]

Name: Anti-Tryptophan Hydroxylase/TPH antibody [EP1311Y] (ab52954) | Abcam
Brand: Abcam Limited
SKU: AB52954
Price: 340 USD
Availability: InStock
Rating: 1 (5 reviews)

RabMAb
Recombinant
20ul selling size
What is this?

(5 Reviews)

(34 Publications)

Rabbit Recombinant Monoclonal Tryptophan Hydroxylase/TPH antibody. Suitable for IHC-P, WB and reacts with Human, Mouse samples. Cited in 34 publications.

View Alternative Names

TPH, TPRH, TRPH, TPH1, Tryptophan 5-hydroxylase 1, Tryptophan 5-monooxygenase 1

4 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tryptophan Hydroxylase/TPH antibody [EP1311Y] (AB52954)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tryptophan Hydroxylase/TPH antibody [EP1311Y] (AB52954)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human bladder carcinoma tissue sections labeling Tryptophan Hydroxylase/TPH with Purified ab52954 at 1 : 2000 dilution (1.191 µg/mL). Heat mediated antigen retrieval was performed using Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0). Tissue was counterstained with Hematoxylin. ImmunoHistoProbe one step HRP Polymer (ready to use) secondary antibody was used. PBS instead of the primary antibody was used as the negative control.

Lab

Western blot - Anti-Tryptophan Hydroxylase/TPH antibody [EP1311Y] (AB52954)

Blocking/Dilution buffer : 5% NFDM/TBST.

TPH1 is mainly expressed in non-neuronal peripheral tissues like pineal gland.

All lanes:

Western blot - Anti-Tryptophan Hydroxylase/TPH antibody [EP1311Y] (ab52954) at 1/1000 dilution

Lane 1:

Human small intestine tissue lysate at 20 µg

Lane 2:

Human pituitary tissue lysate at 20 µg

Lane 3:

Mouse brain tissue lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 51 kDa

Observed band size: 51 kDa

false

Exposure time: 180s

Lab

Western blot - Anti-Tryptophan Hydroxylase/TPH antibody [EP1311Y] (AB52954)

Blocking/Dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-Tryptophan Hydroxylase/TPH antibody [EP1311Y] (ab52954) at 1/1000 dilution

All lanes:

Full-length Tph1 mouse recombinant protein with C-terminal MYC/DDK tag 10 ng

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 51 kDa

Observed band size: 60 kDa

false

Exposure time: 3s

Lab

Western blot - Anti-Tryptophan Hydroxylase/TPH antibody [EP1311Y] (AB52954)

Blocking and diluting buffer and concentration : 5% NFDM/TBST

All lanes:

Western blot - Anti-Tryptophan Hydroxylase/TPH antibody [EP1311Y] (ab52954) at 1/1000 dilution

All lanes:

N-His tagged human Tryptophan Hydroxylase/TPH (aa 105 to 420) recombinant protein at 0.01 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 51 kDa

Observed band size: 35 kDa

false

Exposure time: 3s

HRP

HRP Anti-Tryptophan Hydroxylase/TPH antibody [EP1311Y]
Carrier free

Anti-Tryptophan Hydroxylase/TPH antibody [EP1311Y] - BSA and Azide free
578 PE

PE Anti-Tryptophan Hydroxylase/TPH antibody [EP1311Y]
519 Alexa Fluor® 488

Alexa Fluor® 488 Anti-Tryptophan Hydroxylase/TPH antibody [EP1311Y]
565 Alexa Fluor® 555

Alexa Fluor® 555 Anti-Tryptophan Hydroxylase/TPH antibody [EP1311Y]
665 Alexa Fluor® 647

Alexa Fluor® 647 Anti-Tryptophan Hydroxylase/TPH antibody [EP1311Y]
617 Alexa Fluor® 594

Alexa Fluor® 594 Anti-Tryptophan Hydroxylase/TPH antibody [EP1311Y]
660 APC

APC Anti-Tryptophan Hydroxylase/TPH antibody [EP1311Y]
775 Alexa Fluor® 750

Alexa Fluor® 750 Anti-Tryptophan Hydroxylase/TPH antibody [EP1311Y]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EP1311Y

Isotype

IgG

Carrier free

Reacts with

Human, Mouse

Applications

IHC-P, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Specificity

The mouse recommendation is based on the WB results. We do not guarantee IHC-P for mouse

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/2000", "IHCP-species-notes": "The mouse recommendation is based on the WB results. We do not guarantee IHC-P for mouse.For the unpurified format use at 1/50 dilution Perform heat-mediated antigen retrieval before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500", "WB-species-notes": "" }, "Mouse": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500", "WB-species-notes": "" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Storage information

Stable for 12 months at -20°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Tryptophan Hydroxylase (TPH) is an enzyme that plays a critical mechanical role in the body. It is sometimes known as TPH or anti TPH. The enzyme catalyzes the conversion of tryptophan to 5-hydroxytryptophan the first and rate-limiting step in the biosynthesis of serotonin. The molecular mass of TPH is approximately 55 kDa. TPH has two isoforms TPH1 and TPH2 which are expressed in different tissues. TPH1 is mainly found in peripheral tissues such as the gut pineal gland and thymus while TPH2 is expressed in the central nervous system particularly in the raphe nuclei of the brainstem.

Biological function summary

Tryptophan hydroxylase serves an important role in serotonin production which impacts mood regulation sleep and other functions. TPH operates primarily as a homotetramer and requires biopterin oxygen and iron for its activity. Serotonin synthesized as a result acts as a neurotransmitter and hormone influencing various physiological processes. The differential expression of TPH1 and TPH2 allows for compartmentalized regulation of serotonin synthesis addressing both peripheral and central nervous system needs.

Pathways

TPH is central in the serotonin biosynthetic pathway a critical pathway for neuromodulation. It converts tryptophan into 5-hydroxytryptophan which is then decarboxylated to serotonin. TPH's function connects closely with other proteins in these pathways such as Aromatic L-amino acid decarboxylase which further processes 5-hydroxytryptophan into serotonin. This integration within the serotonin pathway highlights its importance in maintaining serotonin levels and consequently normal neurological function.

Tryptophan hydroxylase has associations with mood disorders and irritable bowel syndrome (IBS). Altered TPH activity may lead to disruptions in serotonin levels influencing development and progression of these conditions. Studies often link TPH2 with depression and anxiety due to its central neural expression and role in serotonin synthesis. Meanwhile TPH1 expressed in peripheral tissues has connections to IBS where serotonin significantly affects gut motility and sensitivity. The relationship between these disorders and TPH offers insights into therapeutic targets to modulate serotonin levels for treatment strategies.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Oxidizes L-tryptophan to 5-hydroxy-l-tryptophan in the rate-determining step of serotonin biosynthesis.

See full target information TPH1

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Publications (34)

Recent publications for all applications. Explore the full list and refine your search

The Journal of reproduction and development 71:256-265 PubMed40754453

2025

Tryptophan promotes sperm hyperactivation in hamsters via 5-hydroxytryptamine biosynthesis within sperm.

Applications

Unspecified application

Species

Unspecified reactive species

Issei Suzuki,Masakatsu Fujinoki,Takao Kamai

Translational pediatrics 14:1213-1229 PubMed40688229

2025

Chenodeoxycholic acid activates the TGR5/TRPA1-5-HT pathway to regulate intestinal motility in breastfed infants and mouse models.

Applications

Unspecified application

Species

Unspecified reactive species

Zihong Shan,Chenghui He,Qingyao Yang,Jingjing Xiong,Meng Li,Mingyao Meng,Yongkun Huang

International journal of molecular sciences 26: PubMed40362261

2025

Restraint Stress Disrupted Intestinal Homeostasis via 5-HT/HTR7/Wnt/β-Catenin/NF-kB Signaling.

Applications

Unspecified application

Species

Unspecified reactive species

Jiayu Yu,Zixu Wang,Yaoxing Chen,Yulan Dong

Microbiome 13:97 PubMed40221776

2025

Cognitive and behavioral benefits of 2'-fucosyllactose in growing mice: the roles of 5-hydroxytryptophan and gut microbiota.

Applications

Unspecified application

Species

Unspecified reactive species

Liuying Zhu,Zhiyi Zhang,Ting Luo,Hongyan Li,Zeyuan Deng,Jing Li,Liufeng Zheng,Jinqiang Liao,Minghui Wang,Bing Zhang

Gut microbes 17:2447834 PubMed39782002

2025

Gut-derived lactic acid enhances tryptophan to 5-hydroxytryptamine in regulation of anxiety via .

Applications

Unspecified application

Species

Unspecified reactive species

Miaomiao Pan,Chenglang Qian,Shaoye Huo,Yuchen Wu,Xinyi Zhao,Yueming Ying,Boyu Wang,Hao Yang,Anaguli Yeerken,Tongyao Wang,Mengwei Fu,Lihong Wang,Yuhuan Wei,Yunhua Zhao,Chunhai Shao,Huijing Wang,Chao Zhao

Journal of neuroinflammation 21:307 PubMed39609821

2024

Taprenepag restores maternal-fetal interface homeostasis for the treatment of neurodevelopmental disorders.

Applications

Unspecified application

Species

Unspecified reactive species

Kai Wang,Shufen Zhang,Yunxia Wang,Xiaomei Wu,Lijuan Wen,Tingting Meng,Xiangyu Jin,Sufen Li,Yiling Hong,Jia Ke,Yichong Xu,Hong Yuan,Fuqiang Hu

Biological research 57:87 PubMed39574138

2024

Depression like-behavior and memory loss induced by methylglyoxal is associated with tryptophan depletion and oxidative stress: a new in vivo model of neurodegeneration.

Applications

Unspecified application

Species

Unspecified reactive species

Md Samsuzzaman,Seong-Min Hong,Jae Hyuk Lee,Hyunjun Park,Keun-A Chang,Hyun-Bum Kim,Myoung Gyu Park,Hyeyoon Eo,Myung Sook Oh,Sun Yeou Kim

Gut microbes 16:2423040 PubMed39569932

2024

ameliorates high-fat diet-induced obesity through modulating intestinal serotonin synthesis and lipid absorption in mice.

Applications

Unspecified application

Species

Unspecified reactive species

Xinxin Wen,Xiaoyan Feng,Fang Xin,Rui An,Huanwei Huang,Liyuan Mao,Ping Liu,Jin Zhang,Haixia Huang,Xicheng Liu,Wei Wang

Alzheimer's & dementia (New York, N. Y.) 7:e12231 PubMed35005208

2021

Novelty-like activation of locus coeruleus protects against deleterious human pretangle tau effects while stress-inducing activation worsens its effects.

Applications

Unspecified application

Species

Unspecified reactive species

Tamunotonye Omoluabi,Sarah E Torraville,Aida Maziar,Abhinaba Ghosh,Kyron D Power,Camila Reinhardt,Carolyn W Harley,Qi Yuan

Scientific reports 11:20376 PubMed34645867

2021

Increased release of serotonin from rat primary isolated adult cardiac myofibroblasts.

Applications

Unspecified application

Species

Unspecified reactive species

Emiri Tarbit,Indu Singh,Jason Nigel Peart,Svetlana Bivol,Roselyn Barbara Rose'Meyer

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Tryptophan Hydroxylase/TPH antibody [EP1311Y]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tryptophan Hydroxylase/TPH antibody [EP1311Y] (AB52954)

Western blot - Anti-Tryptophan Hydroxylase/TPH antibody [EP1311Y] (AB52954)

Western blot - Anti-Tryptophan Hydroxylase/TPH antibody [EP1311Y] (AB52954)

Western blot - Anti-Tryptophan Hydroxylase/TPH antibody [EP1311Y] (AB52954)

Related conjugates and formulations (9)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Specificity

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (34)

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