Rabbit Recombinant Monoclonal Tryptophan Hydroxylase/TPH antibody. Carrier free. Suitable for IHC-P, WB and reacts with Human, Mouse, Recombinant fragment - Human, Recombinant fragment - Mouse samples. Cited in 9 publications.
Images
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tryptophan Hydroxylase/TPH antibody [EP1311Y] - BSA and Azide free (AB227845), expandable thumbnail](https://content.abcam.com/products/images/tryptophan-hydroxylase-tph-antibody-ep1311y-bsa-and-azide-free-ab227845--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img183738.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tryptophan Hydroxylase/TPH antibody [EP1311Y] - BSA and Azide free (AB227845)")
![Western blot - Anti-Tryptophan Hydroxylase/TPH antibody [EP1311Y] - BSA and Azide free (AB227845), expandable thumbnail](https://content.abcam.com/products/images/tryptophan-hydroxylase-tph-antibody-ep1311y-bsa-and-azide-free-ab227845--western-blot-img176182.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Tryptophan Hydroxylase/TPH antibody [EP1311Y] - BSA and Azide free (AB227845)")
Publications
Filter by
- Amino acids 48:2411-212016A novel role for antizyme inhibitor 2 as a regulator of serotonin and histamine biosynthesis and content in mouse mast cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesCarlos Acosta-Andrade et. al.PubMed 27084713
- Physiological reports 3:2015Effect of daytime-restricted feeding in the daily variations of liver metabolism and blood transport of serotonin in rat.Applications:WB, IHCReactive species:Unspecified reactive speciesMarlen Valdés-Fuentes,Gabriela Vera-Rivera,Dalia De Ita-Pérez,Isabel Méndez,María Isabel Miranda,Mauricio Díaz-MuñozPubMed 25948822
- Molecular biology reports 42:1295-3072015Functional constituents of a local serotonergic system, intrinsic to the human coronary artery smooth muscle cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesKannan Baskar et. al.PubMed 25861735
- PloS one 9:e885072014Quiescent and proliferative fibroblasts exhibit differential p300 HAT activation through control of 5-methoxytryptophan production.
- Cardiovascular research 99:24-342013Dexfenfluramine and the oestrogen-metabolizing enzyme CYP1B1 in the development of pulmonary arterial hypertension.
- Cardiovascular research 96:109-192012Developmental basis for filamin-A-associated myxomatous mitral valve disease.
- FASEB journal : official publication of the Federa 26:2457-702012Blunted epidermal L-tryptophan metabolism in vitiligo affects immune response and ROS scavenging by Fenton chemistry, part 1: Epidermal H2O2/ONOO(-)-mediated stress abrogates tryptophan hydroxylase and dopa decarboxylase activities, leading to low serotonin and melatonin levels.
- Anticancer research 31:1153-602011The effect of PTEN on serotonin synthesis and secretion from the carcinoid cell line BON.Applications:WBReactive species:HumanScott R Silva,Yekaterina Y Zaytseva,Lindsey N Jackson,Eun Y Lee,Heidi L Weiss,Kanika A Bowen,Courtney M Townsend,B Mark EversPubMed 21508359
- Muscle & nerve 39:634-412009Expression of synemin in the mouse spinal cord.Applications:IHC-PReactive species:MouseYuji Mizuno,Jeffrey R Guyon,Koichi Okamoto,Louis M KunkelPubMed 19229966
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IHC-P | WB | |
|---|---|---|
| Human | Tested | Tested |
| Mouse | Expected | Tested |
| Recombinant fragment - Human | Not recommended | Tested |
| Recombinant fragment - Mouse | Not recommended | Tested |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes The mouse recommendation is based on the WB results. We do not guarantee IHC-P for mouse. Perform heat-mediated antigen retrieval before commencing with IHC staining protocol. |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Recombinant fragment - Human, Recombinant fragment - Mouse | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Recombinant fragment - Human, Human, Recombinant fragment - Mouse, Mouse | Dilution info - | Notes - |
Associated Products
Select an associated product type
4 products for Alternative Product
1 product for Alternative Version
Target data
Function
Oxidizes L-tryptophan to 5-hydroxy-l-tryptophan in the rate-determining step of serotonin biosynthesis.
Alternative names
TPH, TPRH, TRPH, TPH1, Tryptophan 5-hydroxylase 1, Tryptophan 5-monooxygenase 1
Recommended products
Rabbit Recombinant Monoclonal Tryptophan Hydroxylase/TPH antibody. Carrier free. Suitable for IHC-P, WB and reacts with Human, Mouse, Recombinant fragment - Human, Recombinant fragment - Mouse samples. Cited in 9 publications.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
- Yes
- Clone number
- EP1311Y
- Purification technique
- Affinity purification Protein A
- Specificity
The mouse recommendation is based on the WB results. We do not guarantee IHC-P for mouse.
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- Do Not Freeze
Notes
ab227845 is the carrier-free version of Anti-Tryptophan Hydroxylase/TPH antibody [EP1311Y] ab52954.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Tryptophan Hydroxylase (TPH) is an enzyme that plays a critical mechanical role in the body. It is sometimes known as TPH or anti TPH. The enzyme catalyzes the conversion of tryptophan to 5-hydroxytryptophan the first and rate-limiting step in the biosynthesis of serotonin. The molecular mass of TPH is approximately 55 kDa. TPH has two isoforms TPH1 and TPH2 which are expressed in different tissues. TPH1 is mainly found in peripheral tissues such as the gut pineal gland and thymus while TPH2 is expressed in the central nervous system particularly in the raphe nuclei of the brainstem.
- Biological function summary
Tryptophan hydroxylase serves an important role in serotonin production which impacts mood regulation sleep and other functions. TPH operates primarily as a homotetramer and requires biopterin oxygen and iron for its activity. Serotonin synthesized as a result acts as a neurotransmitter and hormone influencing various physiological processes. The differential expression of TPH1 and TPH2 allows for compartmentalized regulation of serotonin synthesis addressing both peripheral and central nervous system needs.
- Pathways
TPH is central in the serotonin biosynthetic pathway a critical pathway for neuromodulation. It converts tryptophan into 5-hydroxytryptophan which is then decarboxylated to serotonin. TPH's function connects closely with other proteins in these pathways such as Aromatic L-amino acid decarboxylase which further processes 5-hydroxytryptophan into serotonin. This integration within the serotonin pathway highlights its importance in maintaining serotonin levels and consequently normal neurological function.
- Associated diseases and disorders
Tryptophan hydroxylase has associations with mood disorders and irritable bowel syndrome (IBS). Altered TPH activity may lead to disruptions in serotonin levels influencing development and progression of these conditions. Studies often link TPH2 with depression and anxiety due to its central neural expression and role in serotonin synthesis. Meanwhile TPH1 expressed in peripheral tissues has connections to IBS where serotonin significantly affects gut motility and sensitivity. The relationship between these disorders and TPH offers insights into therapeutic targets to modulate serotonin levels for treatment strategies.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
2 product images
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tryptophan Hydroxylase/TPH antibody [EP1311Y] - BSA and Azide free (ab227845), expandable thumbnail](https://content.abcam.com/products/images/tryptophan-hydroxylase-tph-antibody-ep1311y-bsa-and-azide-free-ab227845--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img183738.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tryptophan Hydroxylase/TPH antibody [EP1311Y] - BSA and Azide free (ab227845)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tryptophan Hydroxylase/TPH antibody [EP1311Y] - BSA and Azide free (ab227845)
This data was developed using Anti-Tryptophan Hydroxylase/TPH antibody [EP1311Y] ab52954, the same antibody clone in a different buffer formulation.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human bladder carcinoma tissue sections labeling Tryptophan Hydroxylase/TPH with Purified Anti-Tryptophan Hydroxylase/TPH antibody [EP1311Y] ab52954 at 1:2000 dilution (1.191 µg/mL). Heat mediated antigen retrieval was performed using Perform heat mediated antigen retrieval using Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) ab93684 (Tris/EDTA buffer, pH 9.0). Tissue was counterstained with Hematoxylin. ImmunoHistoProbe one step HRP Polymer (ready to use) secondary antibody was used. PBS instead of the primary antibody was used as the negative control.![Western blot - Anti-Tryptophan Hydroxylase/TPH antibody [EP1311Y] - BSA and Azide free (ab227845), expandable thumbnail](https://content.abcam.com/products/images/tryptophan-hydroxylase-tph-antibody-ep1311y-bsa-and-azide-free-ab227845--western-blot-img176182.jpg "Western blot - Anti-Tryptophan Hydroxylase/TPH antibody [EP1311Y] - BSA and Azide free (ab227845)")
Western blot - Anti-Tryptophan Hydroxylase/TPH antibody [EP1311Y] - BSA and Azide free (ab227845)
Blocking and diluting buffer and concentration: 5% NFDM/TBST
This data was developed using Anti-Tryptophan Hydroxylase/TPH antibody [EP1311Y] ab52954, the same antibody clone in a different buffer formulation.
All lanes: Western blot - Anti-Tryptophan Hydroxylase/TPH antibody [EP1311Y] (Anti-Tryptophan Hydroxylase/TPH antibody [EP1311Y] ab52954) at 1/1000 dilution
All lanes: N-His tagged human Tryptophan Hydroxylase/TPH (aa 105 to 420) recombinant protein at 0.01 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution
Predicted band size: 51 kDa
Observed band size: 35 kDa
Exposure time: 3s
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com