Skip to main content

Rabbit Recombinant Monoclonal Tryptophan Hydroxylase/TPH antibody. Carrier free. Suitable for IHC-P, WB and reacts with Human, Mouse, Recombinant fragment - Human, Recombinant fragment - Mouse samples. Cited in 9 publications.

Be the first to review this product! Submit a review

Images

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

pH: 7.2 - 7.4
Constituents: PBS

Form

Liquid

Clonality

Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.
Loading...
Loading...
Loading...
Loading...
Loading...
Loading...
Loading...
Loading...

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IHC-PWB
Human
Tested
Tested
Mouse
Expected
Tested
Recombinant fragment - Human
Not recommended
Tested
Recombinant fragment - Mouse
Not recommended
Tested

Tested
Tested

Species

Human

Dilution info

-

Notes

The mouse recommendation is based on the WB results. We do not guarantee IHC-P for mouse.

Perform heat-mediated antigen retrieval before commencing with IHC staining protocol.

Expected
Expected

Species

Mouse

Dilution info

Use at an assay dependent concentration.

Notes

-

Not recommended
Not recommended

Species

Recombinant fragment - Human, Recombinant fragment - Mouse

Dilution info

-

Notes

-

Tested
Tested

Species

Recombinant fragment - Human, Human, Recombinant fragment - Mouse, Mouse

Dilution info

-

Notes

-

Target data

Function

Oxidizes L-tryptophan to 5-hydroxy-l-tryptophan in the rate-determining step of serotonin biosynthesis.

Alternative names

Recommended products

  1. Loading...
  2. Loading...
  3. Loading...
  4. Loading...

Rabbit Recombinant Monoclonal Tryptophan Hydroxylase/TPH antibody. Carrier free. Suitable for IHC-P, WB and reacts with Human, Mouse, Recombinant fragment - Human, Recombinant fragment - Mouse samples. Cited in 9 publications.

Alternative names

Key facts

Isotype

IgG

Form

Liquid

Clonality

Monoclonal

Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Carrier free

Yes

Clone number

EP1311Y

Purification technique

Affinity purification Protein A

Specificity

The mouse recommendation is based on the WB results. We do not guarantee IHC-P for mouse.

Concentration
Loading...

Storage

Shipped at conditions

Blue Ice

Appropriate long-term storage conditions

+4°C

Storage information

Do Not Freeze

Notes

ab227845 is the carrier-free version of ab52954.

Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

Supplementary info

Biological function summary

Tryptophan hydroxylase serves an important role in serotonin production which impacts mood regulation sleep and other functions. TPH operates primarily as a homotetramer and requires biopterin oxygen and iron for its activity. Serotonin synthesized as a result acts as a neurotransmitter and hormone influencing various physiological processes. The differential expression of TPH1 and TPH2 allows for compartmentalized regulation of serotonin synthesis addressing both peripheral and central nervous system needs.

Activity summary

Tryptophan Hydroxylase (TPH) is an enzyme that plays a critical mechanical role in the body. It is sometimes known as TPH or anti TPH. The enzyme catalyzes the conversion of tryptophan to 5-hydroxytryptophan the first and rate-limiting step in the biosynthesis of serotonin. The molecular mass of TPH is approximately 55 kDa. TPH has two isoforms TPH1 and TPH2 which are expressed in different tissues. TPH1 is mainly found in peripheral tissues such as the gut pineal gland and thymus while TPH2 is expressed in the central nervous system particularly in the raphe nuclei of the brainstem.

Pathways

TPH is central in the serotonin biosynthetic pathway a critical pathway for neuromodulation. It converts tryptophan into 5-hydroxytryptophan which is then decarboxylated to serotonin. TPH's function connects closely with other proteins in these pathways such as Aromatic L-amino acid decarboxylase which further processes 5-hydroxytryptophan into serotonin. This integration within the serotonin pathway highlights its importance in maintaining serotonin levels and consequently normal neurological function.

Associated diseases and disorders

Tryptophan hydroxylase has associations with mood disorders and irritable bowel syndrome (IBS). Altered TPH activity may lead to disruptions in serotonin levels influencing development and progression of these conditions. Studies often link TPH2 with depression and anxiety due to its central neural expression and role in serotonin synthesis. Meanwhile TPH1 expressed in peripheral tissues has connections to IBS where serotonin significantly affects gut motility and sensitivity. The relationship between these disorders and TPH offers insights into therapeutic targets to modulate serotonin levels for treatment strategies.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

2 product images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tryptophan Hydroxylase/TPH antibody [EP1311Y] - BSA and Azide free (ab227845), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tryptophan Hydroxylase/TPH antibody [EP1311Y] - BSA and Azide free (ab227845)

    This data was developed using ab52954, the same antibody clone in a different buffer formulation.
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human bladder carcinoma tissue sections labeling Tryptophan Hydroxylase/TPH with Purified ab52954 at 1:2000 dilution (1.191 µg/mL). Heat mediated antigen retrieval was performed using Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0). Tissue was counterstained with Hematoxylin. ImmunoHistoProbe one step HRP Polymer (ready to use) secondary antibody was used. PBS instead of the primary antibody was used as the negative control.

  • Western blot - Anti-Tryptophan Hydroxylase/TPH antibody [EP1311Y] - BSA and Azide free (ab227845), expandable thumbnail

    Western blot - Anti-Tryptophan Hydroxylase/TPH antibody [EP1311Y] - BSA and Azide free (ab227845)

    Blocking and diluting buffer and concentration: 5% NFDM/TBST

    This data was developed using ab52954, the same antibody clone in a different buffer formulation.

    All lanes: Western blot - Anti-Tryptophan Hydroxylase/TPH antibody [EP1311Y] (AB52954) at 1/1000 dilution

    All lanes: N-His tagged human Tryptophan Hydroxylase/TPH (aa 105 to 420) recombinant protein at 0.01 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (AB97051) at 1/20000 dilution

    Predicted band size: 51 kDa

    Observed band size: 35 kDa

    Exposure time: 3s

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

There was a problem

We can’t download that datasheet. Please try again. If you need help, contact our Customer Services team at technical@abcam.com