AB234748

Anti-Tryptophan rich protein antibody

Name: Anti-Tryptophan rich protein antibody (ab234748) | Abcam
Brand: Abcam Limited
SKU: AB234748

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(1 Publication)

Rabbit Polyclonal Tryptophan rich protein antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Mouse, Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human GET1 aa 1-100.

View Alternative Names

CHD5, WRB, GET1, Guided entry of tail-anchored proteins factor 1, Congenital heart disease 5 protein, Tail-anchored protein insertion receptor WRB, Tryptophan-rich basic protein

3 Images

Immunocytochemistry/ Immunofluorescence - Anti-Tryptophan rich protein antibody (AB234748)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Tryptophan rich protein antibody (AB234748)

HeLa (human epithelial cell line from cervix adenocarcinoma) cells labeling Tryptophan rich protein (green) using ab234748 at 1/100 dilution in ICC/IF, followed by Alexa Fluor 488^® congugated Goat Anti-Rabbit IgG (H+L).

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tryptophan rich protein antibody (AB234748)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tryptophan rich protein antibody (AB234748)

Paraffin-embedded human testis tissue stained for Tryptophan rich protein using ab234748 at 1/100 dilution in immunohistochemical analysis.

Supplier Data

Western blot - Anti-Tryptophan rich protein antibody (AB234748)

All lanes:

Western blot - Anti-Tryptophan rich protein antibody (ab234748) at 1/2000 dilution

All lanes:

Mouse spleen lysate

Secondary

All lanes:

Goat polyclonal to rabbit IgG at 1/50000 dilution

Predicted band size: 19 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Human

Applications

IHC-P, WB, ICC/IF

applications

Immunogen

Recombinant Fragment Protein within Human GET1 aa 1-100. The exact immunogen used to generate this antibody is proprietary information.

O00258

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/20 - 1/200", "IHCP-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/50 - 1/200", "ICCIF-species-notes": "" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000 - 1/5000", "WB-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein G

Purification notes

Purity >95%.

Storage buffer

pH: 7.4 Preservative: 0.03% Proclin 300 Constituents: PBS, 50% Glycerol (glycerin, glycerine)

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Tryptophan-rich proteins mechanically function as structural components and cellular signaling molecules. Known also by alternate names depending on their specific forms or isoforms these proteins exhibit varying masses often around 15 kDa though the mass can differ among species. One notable example is found in chickens expressed primarily within specialized cells and tissues indicating roles in cell signaling and tissue-specific processes. Various isoforms can be identified in different organisms with expression patterns linked to their specific physiological functions.

Biological function summary

Tryptophan-rich proteins contribute to processes like immune response modulation and cell adhesion impacting cellular communication and structural integrity. These proteins sometimes form part of larger protein complexes enhancing their stability and function in the cellular environment. Tryptophan-rich proteins interact with a spectrum of cellular receptors and other proteins thereby influencing cascade reactions essential for cell maintenance and signal transduction.

Pathways

Research identifies tryptophan-rich proteins as integral components of immune signaling and adhesion-related pathways. They often engage with proteins like integrins important in linking extracellular matrices to the cytoskeleton. Such engagement facilitates cellular communication and migration ensuring proper immune response and tissue maintenance. Interactions with these pathways highlight the protein's importance in maintaining physiological balance and cellular integrity.

Tryptophan-rich proteins hold links to conditions such as autoimmune diseases and chronic inflammation. Aberrations in these proteins can lead to dysregulated immune responses contributing to the pathogenesis of autoimmune conditions. Connections to proteins like cytokines which regulate inflammation elucidate their role in chronic inflammatory states. Understanding their function and interaction with disease-related pathways holds potential for developing targeted therapies.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

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Target data

Required for the post-translational delivery of tail-anchored (TA) proteins to the endoplasmic reticulum (ER) (PubMed : 21444755, PubMed : 23041287, PubMed : 24392163, PubMed : 27226539). Together with CAMLG/GET2, acts as a membrane receptor for soluble GET3/TRC40, which recognizes and selectively binds the transmembrane domain of TA proteins in the cytosol (PubMed : 21444755, PubMed : 23041287, PubMed : 24392163, PubMed : 27226539). Required to ensure correct topology and ER insertion of CAMLG (PubMed : 31417168, PubMed : 32187542).

See full target information GET1

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Advanced science (Weinheim, Baden-Wurttemberg, Germany) 12:e2412233 PubMed39680752

2024

SREBP1c-Mediated Transcriptional Repression of YME1L1 Contributes to Acute Kidney Injury by Inducing Mitochondrial Dysfunction in Tubular Epithelial Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Wang Xin,Jie Zhou,Yuzhu Peng,Shuiqin Gong,Wenhao Liao,Yaqin Wang,Xixin Huang,Yang Mao,Mengying Yao,Shaozong Qin,Jiachuan Xiong,Yan Li,Qigang Lan,Yinghui Huang,Jinghong Zhao

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Tryptophan rich protein antibody

Immunocytochemistry/ Immunofluorescence - Anti-Tryptophan rich protein antibody (AB234748)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tryptophan rich protein antibody (AB234748)

Western blot - Anti-Tryptophan rich protein antibody (AB234748)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Alternative Products

Primary Antibodies

Complementary Products

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

Product promise