JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB317156

Anti-Tryptophanyl tRNA synthetase antibody [HL2186]

Be the first to review this product! Submit a review

|

(0 Publication)

Rabbit Monoclonal Tryptophanyl tRNA synthetase/WRS antibody. Suitable for ICC/IF, WB and reacts with Human, Mouse, Zebrafish samples. Immunogen corresponding to Recombinant Fragment Protein within Human WARS1.

View Alternative Names

IFI53, WARS, WRS, WARS1, Interferon-induced protein 53, Tryptophanyl-tRNA synthetase, IFP53, TrpRS, hWRS

6 Images
Immunocytochemistry/ Immunofluorescence - Anti-Tryptophanyl tRNA synthetase antibody [HL2186] (AB317156)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Tryptophanyl tRNA synthetase antibody [HL2186] (AB317156)

ab317156 detects Tryptophanyl tRNA synthetase protein at cytoplasm by immunofluorescent analysis. Sample : A431 cells were fixed in ice-cold MeOH for 5 min. Green : Tryptophanyl tRNA synthetase stained by ab317156 diluted at 1 : 500. Blue : Fluoroshield with DAPI.

Western blot - Anti-Tryptophanyl tRNA synthetase antibody [HL2186] (AB317156)
  • WB

Supplier Data

Western blot - Anti-Tryptophanyl tRNA synthetase antibody [HL2186] (AB317156)

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 ug) were separated by 10% SDS-PAGE and the membrane was blotted with ab317156 diluted at 1 : 1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

All lanes:

Western blot - Anti-Tryptophanyl tRNA synthetase antibody [HL2186] (ab317156) at 1/1000 dilution

Lane 1:

Non-transfected (–) 293T whole cell extracts at 30 µg

Lanes 2 - 3:

WARS shRNA transfected (+) 293T whole cell extracts at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

false

Western blot - Anti-Tryptophanyl tRNA synthetase antibody [HL2186] (AB317156)
  • WB

Supplier Data

Western blot - Anti-Tryptophanyl tRNA synthetase antibody [HL2186] (AB317156)

Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE and the membrane was blotted with ab317156 diluted at 1 : 1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

All lanes:

Western blot - Anti-Tryptophanyl tRNA synthetase antibody [HL2186] (ab317156) at 1/1000 dilution

Lane 1:

A431 whole cell extracts at 30 µg

Lane 2:

HeLa whole cell extracts at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

false

Western blot - Anti-Tryptophanyl tRNA synthetase antibody [HL2186] (AB317156)
  • WB

Supplier Data

Western blot - Anti-Tryptophanyl tRNA synthetase antibody [HL2186] (AB317156)

Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE and the membrane was blotted with ab317156 diluted at 1 : 1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

All lanes:

Western blot - Anti-Tryptophanyl tRNA synthetase antibody [HL2186] (ab317156) at 1/1000 dilution

Lane 1:

293T whole cell extracts at 30 µg

Lane 2:

A431 whole cell extracts at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

false

Western blot - Anti-Tryptophanyl tRNA synthetase antibody [HL2186] (AB317156)
  • WB

Supplier Data

Western blot - Anti-Tryptophanyl tRNA synthetase antibody [HL2186] (AB317156)

Mouse tissue extract (50 ug) was separated by 10% SDS-PAGE and the membrane was blotted with ab317156 diluted at 1 : 1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

All lanes:

Western blot - Anti-Tryptophanyl tRNA synthetase antibody [HL2186] (ab317156) at 1/1000 dilution

All lanes:

Mouse lung tissue extracts at 50 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

false

Western blot - Anti-Tryptophanyl tRNA synthetase antibody [HL2186] (AB317156)
  • WB

Supplier Data

Western blot - Anti-Tryptophanyl tRNA synthetase antibody [HL2186] (AB317156)

Whole zebrafish extract (30 ug) was separated by 10% SDS-PAGE and the membrane was blotted with ab317156 diluted at 1 : 1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

All lanes:

Western blot - Anti-Tryptophanyl tRNA synthetase antibody [HL2186] (ab317156) at 1/1000 dilution

All lanes:

Whole zebrafish extract at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

HL2186

Isotype

IgG

Carrier free

No

Reacts with

Human, Mouse, Zebrafish

Applications

ICC/IF, WB

applications

Immunogen

Recombinant Fragment Protein within Human WARS1.

P23381

style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/500", "ICCIF-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>" }, "Mouse": { "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>" }, "Zebrafish": { "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>" } } }
style
left-column
style
left-column

Properties and storage information

Form
Liquid
Storage buffer
pH: 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Tryptophanyl tRNA synthetase also known as WRS or TrpRS is an enzyme responsible for attaching tryptophan to its corresponding tRNA during protein synthesis. This enzyme is approximately 53 kDa in mass. It ensures the accurate translation of the genetic code in mRNA into proteins by catalyzing the esterification of tryptophan to the tRNA^Trp. WRS is mostly found in the cytoplasm of cells across various tissues contributing to its role in protein biosynthesis.
Biological function summary

WRS is involved in the important process of protein translation ensuring fidelity in the genetic code transfer from RNA to protein. As part of the aminoacyl-tRNA synthetases family WRS helps in maintaining the overall function and stability of the translational apparatus. It is not typically known to be part of any larger protein complex. In addition to its core function in protein synthesis WRS possesses non-canonical roles related to immune response regulation and angiogenesis.

Pathways

WRS plays key roles in the aminoacyl-tRNA biosynthesis and mTOR signaling pathways. These pathways are essential for regulating protein synthesis and cell growth. In the mTOR pathway WRS's function relates to its interaction with proteins involved in cell growth and proliferation. Its roles within these pathways highlight its influence on cellular homeostasis and protein regulation interacting closely with other synthetases and transcription factors.

WRS has associations with certain cancers and autoimmune disorders. Abnormal expression of WRS is observed in various cancer types reflecting its contribution to dysregulated protein synthesis and cell growth. Additionally WRS has been linked to autoimmune diseases like dermatomyositis where its immune-regulatory roles become compromised. In these conditions WRS's interactions with proteins such as interferons illustrate its importance in the pathophysiology of inflammation and immune response.
style
left-column
style
left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

Target data

Catalyzes the attachment of tryptophan to tRNA(Trp) in a two-step reaction : tryptophan is first activated by ATP to form Trp-AMP and then transferred to the acceptor end of the tRNA(Trp).. Isoform 1. Has no angiostatic activity.. T2-TrpRS. Possesses an angiostatic activity but has no aminoacylation activity (PubMed : 11773625, PubMed : 11773626, PubMed : 14630953). Inhibits fluid shear stress-activated responses of endothelial cells (PubMed : 14630953). Regulates ERK, Akt, and eNOS activation pathways that are associated with angiogenesis, cytoskeletal reorganization and shear stress-responsive gene expression (PubMed : 14630953).. Isoform 2. Has an angiostatic activity.
See full target information WARS1
style
left-column
style
left-column
style
right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com