AB228724

Anti-Tryptophanyl tRNA synthetase/WRS antibody

Name: Anti-Tryptophanyl tRNA synthetase/WRS antibody (ab228724) | Abcam
Brand: Abcam Limited
SKU: AB228724
Price: 525 USD
Availability: InStock
Rating: 4 (1 reviews)

(1 Review)

(3 Publications)

Rabbit Polyclonal Tryptophanyl tRNA synthetase/WRS antibody. Suitable for WB, IHC-P and reacts with Human, Mouse, Rat samples. Cited in 3 publications. Immunogen corresponding to Recombinant Fragment Protein within Human WARS1.

View Alternative Names

IFI53, WARS, WRS, WARS1, Interferon-induced protein 53, Tryptophanyl-tRNA synthetase, IFP53, TrpRS, hWRS

4 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tryptophanyl tRNA synthetase/WRS antibody (AB228724)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tryptophanyl tRNA synthetase/WRS antibody (AB228724)

Paraffin-embedded U87 xenograft tissue stained for Tryptophanyl tRNA synthetase/WRS using ab228724 at 1/500 dilution in immunohistochemical analysis.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tryptophanyl tRNA synthetase/WRS antibody (AB228724)

Paraffin-embedded C2C12 xenograft tissue stained for Tryptophanyl tRNA synthetase/WRS using ab228724 at 1/500 dilution in immunohistochemical analysis.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tryptophanyl tRNA synthetase/WRS antibody (AB228724)

Immunohistochemical analysis of paraffin-embedded rat brain tissue stained for Tryptophanyl tRNA synthetase protein at cytoplasm with ab228724 at a 1/85 dilution.

Antigen retrieval : Citrate buffer, pH 6.0, 15 min

Supplier Data

Western blot - Anti-Tryptophanyl tRNA synthetase/WRS antibody (AB228724)

Samples were separated by 10% SDS-PAGE. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

All lanes:

Western blot - Anti-Tryptophanyl tRNA synthetase/WRS antibody (ab228724) at 1/10000 dilution

Lane 1:

293T whole cell lysates at 30 µg

Lane 2:

WARS shRNA transfected 293T whole cell lysates at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

Predicted band size: 53 kDa

true

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Rat, Human

Applications

IHC-P, WB

applications

Immunogen

Recombinant Fragment Protein within Human WARS1. The exact immunogen used to generate this antibody is proprietary information.

P23381

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/5000 - 1/20000", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/1000", "IHCP-species-notes": "" }, "Mouse": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/1000", "IHCP-species-notes": "" }, "Rat": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/1000", "IHCP-species-notes": "" }, "Chimpanzee": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Cow": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rabbit": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7 Preservative: 0.01% Thimerosal (merthiolate) Constituents: PBS, 20% Glycerol (glycerin, glycerine), 1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Tryptophanyl tRNA synthetase also known as WRS or TrpRS is an enzyme responsible for attaching tryptophan to its corresponding tRNA during protein synthesis. This enzyme is approximately 53 kDa in mass. It ensures the accurate translation of the genetic code in mRNA into proteins by catalyzing the esterification of tryptophan to the tRNA^Trp. WRS is mostly found in the cytoplasm of cells across various tissues contributing to its role in protein biosynthesis.

Biological function summary

WRS is involved in the important process of protein translation ensuring fidelity in the genetic code transfer from RNA to protein. As part of the aminoacyl-tRNA synthetases family WRS helps in maintaining the overall function and stability of the translational apparatus. It is not typically known to be part of any larger protein complex. In addition to its core function in protein synthesis WRS possesses non-canonical roles related to immune response regulation and angiogenesis.

Pathways

WRS plays key roles in the aminoacyl-tRNA biosynthesis and mTOR signaling pathways. These pathways are essential for regulating protein synthesis and cell growth. In the mTOR pathway WRS's function relates to its interaction with proteins involved in cell growth and proliferation. Its roles within these pathways highlight its influence on cellular homeostasis and protein regulation interacting closely with other synthetases and transcription factors.

WRS has associations with certain cancers and autoimmune disorders. Abnormal expression of WRS is observed in various cancer types reflecting its contribution to dysregulated protein synthesis and cell growth. Additionally WRS has been linked to autoimmune diseases like dermatomyositis where its immune-regulatory roles become compromised. In these conditions WRS's interactions with proteins such as interferons illustrate its importance in the pathophysiology of inflammation and immune response.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Catalyzes the attachment of tryptophan to tRNA(Trp) in a two-step reaction : tryptophan is first activated by ATP to form Trp-AMP and then transferred to the acceptor end of the tRNA(Trp).. Isoform 1. Has no angiostatic activity.. T2-TrpRS. Possesses an angiostatic activity but has no aminoacylation activity (PubMed : 11773625, PubMed : 11773626, PubMed : 14630953). Inhibits fluid shear stress-activated responses of endothelial cells (PubMed : 14630953). Regulates ERK, Akt, and eNOS activation pathways that are associated with angiogenesis, cytoskeletal reorganization and shear stress-responsive gene expression (PubMed : 14630953).. Isoform 2. Has an angiostatic activity.

See full target information WARS1

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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Biomolecules & therapeutics 33:852-865 PubMed40798912

2025

Control of Overly Secreted Tryptophanyl tRNA Synthetase Attenuates Sepsis Severity in a Porcine Model.

Applications

Unspecified application

Species

Unspecified reactive species

Yoon Tae Kim,Geunyeol Jeong,Yihyo Kim,HyeonJu Roh,Susung Lim,Jinah Jang,Yun Hui Choi,Kyoung Chul Park,Mirim Jin

EMBO molecular medicine 16:40-63 PubMed38177528

2024

Highly secreted tryptophanyl tRNA synthetase 1 as a potential theranostic target for hypercytokinemic severe sepsis.

Applications

Unspecified application

Species

Unspecified reactive species

Yoon Tae Kim,Jin Won Huh,Yun Hui Choi,Hee Kyeong Yoon,Tram Tt Nguyen,Eunho Chun,Geunyeol Jeong,Sunyoung Park,Sungwoo Ahn,Won-Kyu Lee,Young-Woock Noh,Kyoung Sun Lee,Hee-Sung Ahn,Cheolju Lee,Sang Min Lee,Kyung Su Kim,Gil Joon Suh,Kyeongman Jeon,Sunghoon Kim,Mirim Jin

Cell discovery 9:125 PubMed38114467

2023

Comprehensive genomic profiling of breast cancers characterizes germline-somatic mutation interactions mediating therapeutic vulnerabilities.

Applications

Unspecified application

Species

Unspecified reactive species

Chao Chen,Cai-Jin Lin,Yu-Chen Pei,Ding Ma,Li Liao,Si-Yuan Li,Lei Fan,Gen-Hong Di,Song-Yang Wu,Xi-Yu Liu,Yun-Jin Wang,Qi Hong,Guo-Liang Zhang,Lin-Lin Xu,Bei-Bei Li,Wei Huang,Jin-Xiu Shi,Yi-Zhou Jiang,Xin Hu,Zhi-Ming Shao

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Tryptophanyl tRNA synthetase/WRS antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tryptophanyl tRNA synthetase/WRS antibody (AB228724)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tryptophanyl tRNA synthetase/WRS antibody (AB228724)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tryptophanyl tRNA synthetase/WRS antibody (AB228724)

Western blot - Anti-Tryptophanyl tRNA synthetase/WRS antibody (AB228724)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

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Secondary Antibodies

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Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (3)

Product promise