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Rabbit Recombinant Monoclonal Tryptophanyl tRNA synthetase/WRS antibody. Suitable for WB, ICC/IF, Flow Cyt (Intra) and reacts with Human samples.

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Images

Immunocytochemistry/ Immunofluorescence - Anti-Tryptophanyl tRNA synthetase/WRS antibody [EPR3424] (AB92733), expandable thumbnail
  • Western blot - Anti-Tryptophanyl tRNA synthetase/WRS antibody [EPR3424] (AB92733), expandable thumbnail
  • Flow Cytometry (Intracellular) - Anti-Tryptophanyl tRNA synthetase/WRS antibody [EPR3424] (AB92733), expandable thumbnail

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
WBICC/IFFlow Cyt (Intra)
Human
Tested
Tested
Tested

Tested
Tested

Species
Human
Dilution info
1/1000
Notes

For unpurified use at 1/10000 - 1/100000.

Tested
Tested

Species
Human
Dilution info
1/50
Notes

For unpurified use at 1/100 - 1/250.

Tested
Tested

Species
Human
Dilution info
1/600
Notes

For unpurified use at 1/1000.

Associated Products

Select an associated product type

2 products for Alternative Product

Target data

Function

Catalyzes the attachment of tryptophan to tRNA(Trp) in a two-step reaction: tryptophan is first activated by ATP to form Trp-AMP and then transferred to the acceptor end of the tRNA(Trp). Isoform 1. Has no angiostatic activity. T2-TrpRS. Possesses an angiostatic activity but has no aminoacylation activity (PubMed:11773625, PubMed:11773626, PubMed:14630953). Inhibits fluid shear stress-activated responses of endothelial cells (PubMed:14630953). Regulates ERK, Akt, and eNOS activation pathways that are associated with angiogenesis, cytoskeletal reorganization and shear stress-responsive gene expression (PubMed:14630953). Isoform 2. Has an angiostatic activity.

Alternative names

IFI53, WARS, WRS, WARS1, Interferon-induced protein 53, Tryptophanyl-tRNA synthetase, IFP53, TrpRS, hWRS

Recommended products

Rabbit Recombinant Monoclonal Tryptophanyl tRNA synthetase/WRS antibody. Suitable for WB, ICC/IF, Flow Cyt (Intra) and reacts with Human samples.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
EPR3424
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle, Stable for 12 months at -20°C

Notes

Species reactivity
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species.
Please contact us for more information.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

Tryptophanyl tRNA synthetase also known as WRS or TrpRS is an enzyme responsible for attaching tryptophan to its corresponding tRNA during protein synthesis. This enzyme is approximately 53 kDa in mass. It ensures the accurate translation of the genetic code in mRNA into proteins by catalyzing the esterification of tryptophan to the tRNA^Trp. WRS is mostly found in the cytoplasm of cells across various tissues contributing to its role in protein biosynthesis.

Biological function summary

WRS is involved in the important process of protein translation ensuring fidelity in the genetic code transfer from RNA to protein. As part of the aminoacyl-tRNA synthetases family WRS helps in maintaining the overall function and stability of the translational apparatus. It is not typically known to be part of any larger protein complex. In addition to its core function in protein synthesis WRS possesses non-canonical roles related to immune response regulation and angiogenesis.

Pathways

WRS plays key roles in the aminoacyl-tRNA biosynthesis and mTOR signaling pathways. These pathways are essential for regulating protein synthesis and cell growth. In the mTOR pathway WRS's function relates to its interaction with proteins involved in cell growth and proliferation. Its roles within these pathways highlight its influence on cellular homeostasis and protein regulation interacting closely with other synthetases and transcription factors.

Associated diseases and disorders

WRS has associations with certain cancers and autoimmune disorders. Abnormal expression of WRS is observed in various cancer types reflecting its contribution to dysregulated protein synthesis and cell growth. Additionally WRS has been linked to autoimmune diseases like dermatomyositis where its immune-regulatory roles become compromised. In these conditions WRS's interactions with proteins such as interferons illustrate its importance in the pathophysiology of inflammation and immune response.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

3 product images

  • Immunocytochemistry/ Immunofluorescence - Anti-Tryptophanyl tRNA synthetase/WRS antibody [EPR3424] (ab92733), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-Tryptophanyl tRNA synthetase/WRS antibody [EPR3424] (ab92733)

    Immunocytochemistry analysis of MCF7 (Human breast adenocarcinoma epithelial cell) cells labeling Tryptophanyl tRNA synthetase/WRS with purified ab92733 at 1:50 dilution (10.4 µg/ml). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) at 1/200 dilution (2.5 µg/ml) (Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889) (red). Goat anti-rabbit IgG (Alexa Fluor® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) was used as the secondary antibody at 1:1000 (2 µg/ml) dilution. DAPI (blue) was used as a nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.

  • Western blot - Anti-Tryptophanyl tRNA synthetase/WRS antibody [EPR3424] (ab92733), expandable thumbnail

    Western blot - Anti-Tryptophanyl tRNA synthetase/WRS antibody [EPR3424] (ab92733)

    Full-length TrpRS: 54kDa; mini TrpRS: 48kDa; T1 TrpRS: 47kDa; T2 TrpRS: 44kDa. The molecular weights observed are consistent with what has been described in the literature (PMID: 29666190).

    All lanes: Western blot - Anti-Tryptophanyl tRNA synthetase/WRS antibody [EPR3424] (ab92733) at 1/1000 dilution

    Lane 1: 293T (Human embryonic kidney epithelial cell) whole cell lysate at 20 µg

    Lane 2: HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate at 20 µg

    Lane 3: Human thyroid lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution

    Predicted band size: 53 kDa

  • Flow Cytometry (Intracellular) - Anti-Tryptophanyl tRNA synthetase/WRS antibody [EPR3424] (ab92733), expandable thumbnail

    Flow Cytometry (Intracellular) - Anti-Tryptophanyl tRNA synthetase/WRS antibody [EPR3424] (ab92733)

    Flow Cytometry analysis of HUVEC (Human umbilical vein endothelial cell) treated with 20ng/ml Interferon gamma for 48 hours cells labeling Tryptophanyl tRNA synthetase/WRS with purified ab92733 at 1/1000 dilution (1 μg/mL) (Red). Cells were fixed with 4% Paraformaldehyde and permeabilised with 90% Methanol. A Goat anti rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) secondary antibody was used at 1/2000. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue).
    IFN gamma induction was reported in literature. (PMID: 31324718)

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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