Anti-Tryptophanyl tRNA synthetase/WRS antibody [EPR3424] - BSA and Azide free

• Flow Cyt (Intra)

Lab

Flow Cytometry (Intracellular) - Anti-Tryptophanyl tRNA synthetase/WRS antibody [EPR3424] - BSA and Azide free (AB247596)

This data was developed using ab92733, the same antibody clone in a different buffer formulation.

Flow Cytometry analysis of HUVEC (Human umbilical vein endothelial cell) treated with 20ng/ml Interferon gamma for 48 hours cells labeling Tryptophanyl tRNA synthetase/WRS with purified ab92733 at 1/1000 dilution (1 μg/mL) (Red). Cells were fixed with 4% Paraformaldehyde and permeabilised with 90% Methanol. A Goat anti rabbit IgG (Alexa Fluor^® 488, ab150077) secondary antibody was used at 1/2000. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue).
IFN gamma induction was reported in literature. (PMID : 31324718)

• ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Tryptophanyl tRNA synthetase/WRS antibody [EPR3424] - BSA and Azide free (AB247596)

This data was developed using ab92733, the same antibody clone in a different buffer formulation.
Immunocytochemistry analysis of MCF7 (Human breast adenocarcinoma epithelial cell) cells labeling Tryptophanyl tRNA synthetase/WRS with purified ab92733 at 1 : 50 dilution (10.4 µg/ml). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) at 1/200 dilution (2.5 µg/ml) (ab195889) (red). Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) was used as the secondary antibody at 1 : 1000 (2 µg/ml) dilution. DAPI (blue) was used as a nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.

• WB

Supplier Data

Western blot - Anti-Tryptophanyl tRNA synthetase/WRS antibody [EPR3424] - BSA and Azide free (AB247596)

This data was developed using ab92733, the same antibody clone in a different buffer formulation. Full-length TrpRS : 54kDa; mini TrpRS : 48kDa; T1 TrpRS : 47kDa; T2 TrpRS : 44kDa. The molecular weights observed are consistent with what has been described in the literature (PMID : 29666190).

All lanes:

Western blot - Anti-Tryptophanyl tRNA synthetase/WRS antibody [EPR3424] (<a href='/en-us/products/primary-antibodies/tryptophanyl-trna-synthetase-wrs-antibody-epr3424-ab92733'>ab92733</a>) at 1/1000 dilution

Lane 1:

293T (Human embryonic kidney epithelial cell) whole cell lysate at 20 µg

Lane 2:

HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate at 20 µg

Lane 3:

Human thyroid lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 53 kDa

false